Nonomuraea monospora sp. nov., an actinomycete isolated from cave soil in Thailand, and emended description of the genus Nonomuraea

Abstract

A novel actinomycete, designated strain PT708T, was isolated from cave soil collected in Pha Tup Cave Forest Park, Nan province, Thailand. It produced compounds with antimicrobial and anticancer activities. Its chemotaxonomic properties were consistent with those of members of the genus Nonomuraea . The major menaquinone was MK-9(H4), with minor amounts of MK-9(H6), MK-9(H2), MK-10(H2) and MK-8(H4). The polar lipid profile contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The major fatty acids were iso-C16 : 0, 10-methyl C17 : 0, C16 : 0 and C17 : 1ω6c. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PT708T belonged to the genus Nonomuraea and was most closely related to Nonomuraea rhizophila YIM 67092T (98.50 % sequence similarity) and Nonomuraea rosea GW 12687T (98.30 %). The genomic DNA G+C content of strain PT708T was 73.3 mol%. Unlike the recognized members of the genus Nonomuraea , the novel strain formed single spores at the tips of aerial hyphae. Based on the phenotypic, phylogenetic and genotypic evidence, strain PT708T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea monospora sp. nov. is proposed. The type strain is PT708T ( = TISTR 1910T = JCM 16114T).

The genus Nonomuraea: A review of a rare actinomycete taxon for novel metabolites

“…Recently, we proposed a novel morphology to form single spores on the tip of branched aerial mycelia by N . monospora PT708 T , which differs from the typical morphology describing the genus at this moment . Later on, N .…”

“…As an example of N . monospora PT708 T that was isolated from cave soil in Thailand . At this cave habitat, some pharmaceutical potential Nonomuraea spp.…”

“…Other non‐elucidated anticancer substances have been found in crude ethyl acetate extract of N . monospora PT708 T culture broth, which showed in vitro cytotoxicity to human small lung cancer (NCIH187) and oral cavity cancer (KB) cells with the half maximal inhibitory concentrations (IC 50 ) of 3.48 and 16.11 mg ml −1 , respectively .…”

“…monospora PT708 T sp. nov. . Spore and mycelium morphology of the isolates PT702 (d) and PT708 (f) was observed by scanning electron microscope (SEM) (model JSM‐5910, Japan) after growing them on International Streptomyces Project Medium 4 (ISP4) at 30 °C for 16 days.…”

“…The genus Nonomuraea belongs to the family Streptosporangiaceae of the phylum actinobacteria [6]. Recently, we proposed a novel morphology to form single spores on the tip of branched aerial mycelia by N. monospora PT708 T , which differs from the typical morphology describing the genus at this moment [7,8]. Later on, N. solani NEAU-Z6 T isolated from eggplant root is also able to form single spores [9].…”