Nonlinear hybridization chain reaction-based functional DNA nanostructure assembly for biosensing, bioimaging applications

Zeng, Zhuoer; Zhou, Rong; Sun, Ruowei; Zhang, Xun; Zhang, Cheng; Chen, Chuanpin; Zhu, Qubo

doi:10.1016/j.bios.2020.112814

Cited by 58 publications

(39 citation statements)

References 76 publications

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“…To overcome this limitation, a nonlinear HCR amplification strategy was introduced, which has been used in various analysis methods [132][133][134]. In contrast to general HCR, nonlinear HCR is composed of more complex components, including a trigger DNA sequence, two dsDNA substrates with bridge loops in the middle, and two assistant DNA fragments, which can be assembled into highly branched DNA nanostructures in the presence of target proteins [135]. As a result, a non-linear HCR can achieve better amplification efficiency and larger molecular weight.…”

Section: Other Emerging Amplification Technologiesmentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

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Surface plasmon resonance (SPR) can track molecular interactions in real time, and is a powerful as well as widely used biological and chemical sensing technique. Among the different SPR-based sensing applications, aptamer-based SPR biosensors have attracted significant attention because of their simplicity, feasibility, and low cost for target detection. Continuous developments in SPR aptasensing research have led to the emergence of abundant technical and design concepts. To understand the recent advances in SPR for biosensing, this paper reviews SPR-based research from the last seven years based on different sensing-type strategies and sub-directions. The characteristics of various SPR-based applications are introduced. We hope that this review will guide the development of SPR aptamer sensors for healthcare.

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Section: Other Emerging Amplification Technologiesmentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

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“…Recently, beside linear chain hybridization, many new strategies have been proposed to enhance the detection efficiency (Z. Zeng et al, 2020;J. Zhang, Lakshmipriya, et al, 2020).…”

Section: Hybridization Chain Reactionmentioning

confidence: 99%

Nucleic acid‐based electrochemical biosensor: Recent advances in probe immobilization and signal amplification strategies

Thapa

Liu

Wang

2021

WIREs Nanomed Nanobiotechnol

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With the increasing importance of accurate and early disease diagnosis and the development of personalized medicine, DNA-based electrochemical biosensor has attracted broad scientific and clinical interests in the past decades due to its unique hybridization specificity, fast response time, and potential for miniaturization. In order to achieve high detection sensitivity, the design of DNA electrochemical biosensors depends critically on the improvement of the accessibility of target molecules and the enhancement of signal readout. Here, we summarize the recent advances in DNA probe immobilization and signal amplification strategies with a special focus on DNA nanostructure-supported DNA probe immobilization method, which provides the opportunity to rationally control the distance between probes and keep them in upright confirmation, as well as the contribution of functional nanomaterials in enhancing the signal amplification. The next challenge of biosensors will be the fabrication of point-of-care devices for clinical testing. The advancement of multidisciplinary areas, including nanofabrication, material science, and biochemistry, has exhibited profound promise in achieving such portable sensing devices.

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“…The detecting system is based on the self-assembly of 1D DNA chains formed by bi-functional oligonucleotides forming double stranded dimers on one hand and splitaptamer sequences on the other hand. The use of the hybridization chain reaction (HCR) forming such self-assembled 1D DNA chains for signal amplification has been widely developed in recent years [34][35][36][37][38][39][40]. However, its use with aptamer recognition to form what are called aptachains, is still in its infancy [3,[41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration

Saint-Pierre

Gasparutto

et al. 2021

Biosensors

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Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of adenosine. Aptamers, short oligonucleotides, are known to be particularly efficient probes compared to antibodies thanks to their advantageous probe/target size ratio. Aptachains are formed from dimers with dangling ends followed by the split-aptamer binding triggered by the presence of the target. The high melting temperature of the dimers served as a calibration for the detection/quantification of the target based on the height and/or temperature shift of the aptachain melting peak.

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Nonlinear hybridization chain reaction-based functional DNA nanostructure assembly for biosensing, bioimaging applications

Cited by 58 publications

References 76 publications

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Nucleic acid‐based electrochemical biosensor: Recent advances in probe immobilization and signal amplification strategies

Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration

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