Noninvasive visualization of tumors in rodent dorsal skin window chambers

Huang, Qian; Shan, Siqing; Braun, Rosemary; Lanzen, Jennifer L.; Anyrhambatla, Gopal; Kong, Garheng; Borelli, Michael; Corry, Peter M.; Dewhirst, Mark W.; Li, Chuan-Yuan

doi:10.1038/13736

Cited by 82 publications

(55 citation statements)

References 12 publications

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“…The skinfold chamber, which consists of two symmetrical titanium frames, was implanted in the dorsal skin of the mice following previously reported procedures (40)(41)(42). The chamber sandwiched and immobilized the skin on the back of the mouse.…”

Section: Dorsal Skinfold Chamber Model In Micementioning

confidence: 99%

Label-free DNA imaging in vivo with stimulated Raman scattering microscopy

Lü

Basu

Igras

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

242

210

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Label-free DNA imaging is highly desirable in biology and medicine to perform live imaging without affecting cell function and to obtain instant histological tissue examination during surgical procedures. Here we show a label-free DNA imaging method with stimulated Raman scattering (SRS) microscopy for visualization of the cell nuclei in live animals and intact fresh human tissues with subcellular resolution. Relying on the distinct Raman spectral features of the carbon-hydrogen bonds in DNA, the distribution of DNA is retrieved from the strong background of proteins and lipids by linear decomposition of SRS images at three optimally selected Raman shifts. Based on changes on DNA condensation in the nucleus, we were able to capture chromosome dynamics during cell division both in vitro and in vivo. We tracked mouse skin cell proliferation, induced by drug treatment, through in vivo counting of the mitotic rate. Furthermore, we demonstrated a label-free histology method for human skin cancer diagnosis that provides comparable results to other conventional tissue staining methods such as H&E. Our approach exhibits higher sensitivity than SRS imaging of DNA in the fingerprint spectral region. Compared with spontaneous Raman imaging of DNA, our approach is three orders of magnitude faster, allowing both chromatin dynamic studies and label-free optical histology in real time.

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Section: Dorsal Skinfold Chamber Model In Micementioning

confidence: 99%

Label-free DNA imaging in vivo with stimulated Raman scattering microscopy

Lü

Basu

Igras

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

242

210

View full text Add to dashboard Cite

show abstract

“…This window preserves the exposed opposite surface for observation through a glass coverslip, which is fastened on top of the subcutaneous skin surface. Previous studies have demonstrated that small tumors placed within the dorsal window chamber become vascularized by subcutaneous host blood vessels and undergo rapid growth within 10 -14 days (Huang et al, 1999;Li et al, 2000;Papenfuss et al, 1979). This system enabled us to monitor the angiogenic response of the subcutaneous host blood vessels initiated by tumor-derived signals in live animals by photomicroscopy.…”

Section: Soluble Epha2-fc Receptor Inhibits Rip-tag Tumorinduced Angimentioning

confidence: 99%

“…4T1 cells have been shown to elicit a robust angiogenic response in this assay system (Huang et al, 1999;Li et al, 2000). Hydron pellets (outlined in yellow) impregnated with PBS vehicle, control human IgG, or EphA2-Fc were co-implanted into the dorsal skin fold of mice along with 4T1-GFP cells (green).…”

Section: Soluble Epha2-fc Inhibits 4t1 Tumor Cell-induced Angiogenesismentioning

confidence: 99%

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Soluble Eph A receptors inhibit tumor angiogenesis and progression in vivo

et al. 2002

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The Eph family of receptor tyrosine kinases and their ligands, known as ephrins, play a crucial role in vascular development during embryogenesis. The function of these molecules in adult angiogenesis has not been well characterized. Here, we report that blocking Eph A class receptor activation inhibits angiogenesis in two independent tumor types, the RIP-Tag transgenic model of angiogenesis-dependent pancreatic islet cell carcinoma and the 4T1 model of metastatic mammary adenocarcinoma. Ephrin-A1 ligand was expressed in both tumor and endothelial cells, and EphA2 receptor was localized primarily in tumor-associated vascular endothelial cells. Soluble EphA2-Fc or EphA3-Fc receptors inhibited tumor angiogenesis in cutaneous window assays, and tumor growth in vivo. EphA2-Fc or EphA3-Fc treatment resulted in decreased tumor vascular density, tumor volume, and cell proliferation, but increased cell apoptosis. However, EphA2-Fc had no direct effect on tumor cell growth or apoptosis in culture, yet inhibited migration of endothelial cells in response to tumor cells, suggesting that the soluble receptor inhibited blood vessel recruitment by the tumor. These data provide the first functional evidence for Eph A class receptor regulation of pathogenic angiogenesis induced by tumors and support the function of A class Eph receptors in tumor progression.

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“…Not only hemodynamic studies, but tumor angiogenesis and host tissue with biomaterial interaction studies also use a dorsal skinfold chamber model as a visualization tool of microvessels. 14 Combining the fluorescent dyes with a dorsal skinfold chamber, under a fluorescence intravital microscope, leads to a technique that enhances microvasculature and blood components visualization. Algenstaedt et al applied fluorescein isothiocyanate (FITC)-dextran as a plasma tracer to measure functional vascular density, diameter and blood flow in diabetic mice with a dorsal skinfold chamber.…”

Section: Figure 6 Capillaries Viewed Through a Dorsal Skinfold Chambementioning

confidence: 99%