Intravital microscopy in a dorsal skinfold chamber: hemodynamics, tumor angiogenesis and inflammation

Duansak, Naphatsanan; Chatpun, Surapong

doi:10.7439/ijbr.v4i2.229

Cited by 1 publication

(2 citation statements)

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“…In order to resolve this problem, a dorsal skin chamber window based on surgical operation was developed, which has been widely used in tumor studies and pharmacokinetics , etc. It was well known that surgical operations were always accompanied with bleeding and changes of the normal physiological environment during process of model establishment . Recently, the rapidly developed tissue optical clearing (TOC) technique provides an alternative solution through topical application of optical clearing agents (OCAs) on the dorsal skin, which demonstrated its powerful capacity in optimizing the imaging performances, including the imaging depth, resolution, contrast and sensitivity, of various optical imaging modalities, e. g. laser speckle contrast imaging , photoacoustic microscopy , optical coherence tomography , confocal microscopy , two‐photon microscopy and flow cytometry , etc.…”

Section: Introductionmentioning

confidence: 99%

“…Skin, as the outermost organ of the body, is an inevitable barrier to perform intravital optical imaging, whose strong scattering characteristic limits the penetration of light into tissue. In order to resolve this problem, a dorsal skin chamber window based on surgical operation was developed, which has been widely used in tumor studies and pharmacokinetics , etc. It was well known that surgical operations were always accompanied with bleeding and changes of the normal physiological environment during process of model establishment .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

FSOCA‐induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo

Shi¹,

Feng²,

Zhang³

et al. 2017

Journal of Biophotonics

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The mouse footpad for its feature of hairlessness provides an available window for imaging vascular and cellular structure and function in vivo. Unfortunately, the strong scattering of its skin limits the penetration of light and reduces the imaging contrast and depth. Herein, an innovative footpad skin optical clearing agent (FSOCA) was developed to make the footpad skin transparent quickly by topical application. The results demonstrate that FSOCA treatment not only allowed the cutaneous blood vessels and blood flow distribution to be monitored by laser speckle contrast imaging technique with higher contrast, but also permitted the fluorescent cells to be imaged by laser scanning confocal microscopy with higher fluorescence signal intensity and larger imaging depth. In addition, the physiological saline-treatment could make the footpad skin recover to the initial turbid status, and reclearing would not induce any adverse effects on the distributions and morphologies of blood vessels and cells, which demonstrated a safe and switchable window for biomedical imaging. This switchable footpad skin optical clearing window will be significant for studying blood flow dynamics and cellular immune function in vivo in some vascular and immunological diseases.

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