Noninvasive reflection mode photoacoustic imaging through infant skull toward imaging of neonatal brains

Wang, Xueding; Chamberland, David L.; Xi, Guohua

doi:10.1016/j.jneumeth.2007.11.007

Cited by 51 publications

(38 citation statements)

References 60 publications

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“…Wang et al [169] first studied ex vivo transcranial PAT over a neonatal skull. They used part of a neonatal skull covering fresh canine brain tissue, which has similar optical properties to the human brain.…”

Section: Translational Researchmentioning

confidence: 99%

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Photoacoustic tomography and sensing in biomedicine

2009

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Photoacoustics has been broadly studied in biomedicine, for both human and small animal tissues. Photoacoustics uniquely combines the absorption contrast of light or radio frequency waves with ultrasound resolution. Moreover, it is non-ionizing and non-invasive, and is the fastest growing new biomedical method, with clinical applications on the way. This article provides a brief review of recent developments in photoacoustics in biomedicine, from basic principles to applications. The emphasized areas include the new imaging modalities, hybrid detection methods, photoacoustic contrast agents, and the photoacoustic Doppler effect, as well as translational research topics.

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Section: Translational Researchmentioning

confidence: 99%

“…(c) PA signals from the same vessel at the distance of 21mm. Reprinted from [169], with permission from Elsevier.…”

Section: Figures and Tablesmentioning

confidence: 99%

Photoacoustic tomography and sensing in biomedicine

2009

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“…A skull is a highly scattering medium, attenuating both illumination light and acoustic signals. Although PAT of the brain through a skull was well conducted on monkeys (120; 121) and an infant (122), not until very recently, were the feasibility of imaging through adult skulls demonstrated (115). Since an adult skull is much thicker (~ 7 mm) than a monkey skull or an infant, the attenuation of light becomes more severe — only ~ 2% of photons can pass through (115).…”

Section: Technical Advances From Bench To Bedsidementioning

confidence: 99%

Photoacoustic Microscopy and Computed Tomography: From Bench to Bedside

Wang

Gao

2014

Annu. Rev. Biomed. Eng.

209

183

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Photoacoustic imaging (PAI) of biological tissue has seen immense growth in the past decade, providing unprecedented spatial resolution and functional information at depths in the optical diffusive regime. PAI uniquely combines the advantages of optical excitation and acoustic detection. The hybrid imaging modality features high sensitivity to optical absorption and wide scalability of spatial resolution with the desired imaging depth. Here we first summarize the fundamental principles underpinning the technology, then highlight its practical implementation, and finally discuss recent advances towards clinical translation.

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“…According to a previous study,11 the wavelength-dependent optical attenuation due to the intact skull affects the measurement accuracy. Here, this wavelength dependence was estimated by measuring the photoacoustic signals of a black polyethylene film attached beneath the skull of a freshly sacrificed mouse of the same type at the two operating wavelengths, respectively.…”

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confidence: 98%

Functional transcranial brain imaging by optical-resolution photoacoustic microscopy

et al. 2009

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Abstract. Optical-resolution photoacoustic microscopy ͑OR-PAM͒ is applied to functional brain imaging in living mice. A near-diffraction-limited bright-field optical illumination is employed to achieve micrometer lateral resolution, and a dual-wavelength measurement is utilized to extract the blood oxygenation information. The variation in hemoglobin oxygen saturation ͑sO 2 ͒ along vascular branching has been imaged in a precapillary arteriolar tree and a postcapillary venular tree, respectively. To the best of our knowledge, this is the first report on in vivo volumetric imaging of brain microvascular morphology and oxygenation down to single capillaries through intact mouse skulls. It is anticipated that: ͑i͒ chronic imaging enabled by this minimally invasive procedure will advance the study of cortical plasticity and neurological diseases; ͑ii͒ revealing the neuroactivity-dependent changes in hemoglobin concentration and oxygenation will facilitate the understanding of neurovascular coupling at the capillary level; and ͑iii͒ combining functional OR-PAM and high-resolution blood flowmetry will have the potential to explore cellular pathways of brain energy metabolism. Advances in brain imaging facilitate the understanding of cognitive phenomena and neurological diseases. However, high-resolution brain imaging through intact animal skulls remains challenging for pure optical modalities because the optical scattering and absorption of the skull degrade the imaging resolution and signal-to-noise ratio ͑SNR͒. Photoacoustic imaging, combining light and ultrasound in a single hybrid technology, suggests a potential solution. Using acousticresolution photoacoustic microscopy ͑AR-PAM͒, Stein et al. recently demonstrated mouse brain imaging through both intact scalp and skull.1 This noninvasive feature is highly desirable for functional or chronic studies; however, with its current spatial resolution ͑lateral resolution: 70 m; axial resolution: 54 m͒, capillaries are not resolvable. To fill the gap, Maslov et al. developed optical-resolution photoacoustic microscopy ͑OR-PAM͒ capable of imaging single capillaries in vivo.2 The lateral resolution of OR-PAM matches the size of a single red blood cell ͑RBC͒, and its sensitivity enables single RBC detection. Here, we report on the first demonstration of OR-PAM for functional brain microvascular imaging down to single capillaries through intact mouse skulls. The minimally invasive feature is favorable for chronic study of cortical plasticity. Moreover, because neuronal activity is widely assumed to spatially correlate most closely to the capillary bed response, 4 improving localization of signals down to the capillary level will enable functional brain mapping at micrometer resolution.Before functional brain imaging, a Swiss Webster mouse ͑Hsd:ND4, Harlan Co., 25-30 g͒ was anesthetized by intraperitoneally administering a dose of 87 mg/ kg ketamine and 13 mg/ kg xylazine and transferred to a stereotaxic imaging stage. The scalp of the mouse was surgically removed, and the ex...

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Noninvasive reflection mode photoacoustic imaging through infant skull toward imaging of neonatal brains

Cited by 51 publications

References 60 publications

Photoacoustic tomography and sensing in biomedicine

Photoacoustic tomography and sensing in biomedicine

Photoacoustic Microscopy and Computed Tomography: From Bench to Bedside

Functional transcranial brain imaging by optical-resolution photoacoustic microscopy

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