Noninvasive quantitation of phosphorus metabolites in human tissue by NMR spectroscopy

Roth, Klaus; Hubesch, Bruno; Meyerhoff, Dieter J.; Shoji, Nobuyuki; Gober, Joel R.; Lawry, T.; Boska, Michael D.; Matson, Gerald B.; Weiner, Michael W.

doi:10.1016/0022-2364(89)90062-0

Cited by 102 publications

(74 citation statements)

References 20 publications

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“…A small phantom with known concentration of P i placed beside the image object can be used as an external reference (74). However, a correction factor is needed to account for the differences in field strength, coil sensitivity, and T 1 saturation between the external reference and metabolites.…”

Section: P-mrsmentioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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Many human diseases are caused by an imbalance between energy production and demand.Magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) provide the unique opportunity for in vivo assessment of several fundamental events in tissue metabolism without the use of ionizing radiation. Of particular interest, phosphate metabolites that are involved in ATP generation and utilization can be quantified noninvasively by phosphorous-31 ( 31 P) MRS/MRI. Furthermore, 31 P magnetization transfer (MT) techniques allow in vivo measurement of metabolic fluxes via creatine kinase (CK) and ATP synthase. However, a major impediment for the clinical applications of 31 P-MRS/MRI is the prohibitively long acquisition time and/or the low spatial resolution that are necessary to achieve adequate signal-to-noise ratio. P-MRS/MRI techniques. Applications of these techniques to the investigation of a specific physiology/pathology will be discussed without detailed description. Interested readers are referred to recent review articles on the applications of 31 P-MRS/MRI in metabolic characterization of skeletal muscle (10-12), heart (13), brain (14), and liver (11), as well as in diseases such as cancer (15), heart failure (16), and obesity and diabetes (17,18). Historical perspectiveThe use of 31 P-MRS for metabolic investigations dates back to 1960. Cohn and Hughes were the first to obtain a high-resolution 31 P spectrum of a solution of ATP (19). In addition, they also observed a dependence of the chemical shifts of the phosphorus nuclei of ATP on the pH of the solution. In parallel to the early development of MRI methods by Lauterbur (20), the entire 1970s also witnessed the rapid advance of 31 P-MRS in metabolic investigation of a broad range of biological systems. In 1973, Moon and Richards performed the first 31 P-MRS study that measured intracellular pH in human red blood cells (21). In the following year, Henderson and colleagues obtained the first 31 P spectrum of ATP from human red blood cells (22). At the same time, the Oxford team led by Radda performed the first experiment to acquire 31 P spectra from an intact organ, i.e., the excised and superfused muscle of rat hindlimb (23). The first in vivo 31 P-MRS study was performed on mouse brain by Chance et al. (24). Within the short span of one decade, organelles including mitochondria (25,26) and chromaffin granules (27), cells including Escherichia coli (28), yeast (29), and hepatocytes (30), and organs including skeletal muscle (31,32), heart (33-35), brain (36), kidney (37), and liver (38,39) have all been studied using 31 P-MRS.It is worth noting that most of these organ studies were performed on excised organs to avoid the need for spatial localization. Although Lauterbur has demonstrated the feasibility of imaging water protons (20), it was considered impractical for 31 P imaging of living systems because of the low sensitivity and difficulties with spectral resolution.The 1980s began with the publication of two important studies that aimed a...

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Section: P-mrsmentioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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“…Part of the limited use of 31 P spectroscopy can be attributed to the large range of normal values for cerebral PCr (3-5 mM) and ATP (2-4 mM) in low field studies (59)(60)(61)(62), and the resulting perception that 31 P spectroscopy is highly inaccurate. These initial reports used large volumes of 25-100 cm 3 and did not attempt to account for tissue or regional heterogeneity.…”

Section: Factors Affecting the Interpretation Ofmentioning

confidence: 98%

NMR Studies of Bioenergetic Impairment in Human Epilepsy

Hetherington

Pan

Spencer

2004

Brain Energetics and Neuronal Activity

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“…The first measurements of 31 P metabolites in human brain were made at the University of California at San Francisco (Roth et al, 1989). An internal water concentration of 75 % was assumed.…”

Section: 'The Proportionality Constant [Between the Ratio Of Phosphormentioning

confidence: 99%

Spectroscopy: ¹ H Metabolite Concentrations

Tofts

Waldman

2003

Quantitative MRI of the Brain

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Noninvasive quantitation of phosphorus metabolites in human tissue by NMR spectroscopy

Cited by 102 publications

References 20 publications

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

NMR Studies of Bioenergetic Impairment in Human Epilepsy

Spectroscopy: ¹ H Metabolite Concentrations

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Noninvasive quantitation of phosphorus metabolites in human tissue by NMR spectroscopy

Cited by 102 publications

References 20 publications

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

NMR Studies of Bioenergetic Impairment in Human Epilepsy

Spectroscopy: 1 H Metabolite Concentrations

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Spectroscopy: ¹ H Metabolite Concentrations