Noninvasive Markers of Oxidative DNA Stress, RNA Degradation and Protein Degradation Are Differentially Correlated With Resting Metabolic Rate and Energy Intake in Children and Adolescents

Abstract

Urinary excreted RNA and DNA catabolites are used as noninvasive markers for metabolic processes: 8-oxo-2'-deoxyguanosine (8-oxodG) potentially represents oxidative stress to DNA/deoxyribonucleotidetriphosphate pool, modified ribonucleoside pseudouridine (psi) originating mainly from degraded rRNA and tRNA reflects RNA turnover. Modified amino acid gamma-carboxyglutamic acid (Gla) stems from degraded proteins reflecting turnover of proteins. Aim of the present study was to investigate (44 healthy males, 3-18 y) how excretion rates of 8-oxodG, psi, and Gla are related to resting metabolic rate and energy intake. Excretion rates of 8-oxodG (pmol/kg/d), psi (micromol/kg/d), and Gla (micromol/kg/d) were significantly correlated with resting metabolic rate (kJ/kg/d): r = 0.108 (p = 0.029), 0.691 and 0.552 (p < 0.0001), respectively. Excretion rates of 8-oxodG, psi, and Gla were also significantly correlated with energy intake (kJ/kg/d): r = 0.108 (p = 0.036), 0.602 and 0.462 (p < 0.0001). 8-oxodG and Gla excretion was significantly correlated with psi excretion: r = 0.174 (p = 0.005) and 0.709 (p < 0.0001). These results indicate close relationships between whole-body RNA and protein degradation and metabolic rate. The relationship between 8-oxodG excretion and metabolic rate, however, is less strong suggesting that factors other than metabolic rate considerably affect the oxidative stress to DNA.

Decreased baroreflex sensitivity is linked to sympathovagal imbalance, body fat mass and altered cardiometabolic profile in pre-obesity and obesity

“…In the present study, TBARS had independent association with BRS in obese group subjects (Table 5). From the findings of the present study, it appears that increased oxidative stress in obese subjects could be due to the increased sympathetic drive and increased BM, as there are earlier reports of oxidative stress in conditions of increased sympathetic activity and metabolism [45,46]. Thus, oxidative stress could be the possible link between increased BM and decreased BRS in obesity.…”

Urinary Metabolomics in Pediatric Obesity and NAFLD Identifies Metabolic Pathways/Metabolites Related to Dietary Habits and Gut-Liver Axis Perturbations

“…These metabolic processes, again, could be targeted by oxidative DNA stress in pediatric obesity and obesity-related NAFLD, in conditions such as high dietary energy intake [42]. …”

High basal metabolic rate does not elevate oxidative stress during reproduction in laboratory mice

“…Kim et al (Kim et al, 2010) and studies cited therein]. Interestingly, in non-reproducing females, the level of oxidative damage was higher in L-BMR mice, whereas earlier studies reported positive inter-and intra-specific correlation between the concentration of 8-oxodG and basal and/or standard metabolic rate (Foksinski et al, 2004;Topp et al, 2008).…”