Noninvasive imaging of the lung NETosis by anti-Ly6G iron oxide nanoparticles

Zhong, Jianghong; Zheng, Chanyu; Gao, Haiqiang; Tong, Wei; Hui, Hui; Tian, Jie

doi:10.1016/j.heliyon.2022.e10043

Cited by 3 publications

(6 citation statements)

References 38 publications

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“…In this study, we used intraperitoneal injections of synthetic peptides P140 encompassing the same sequence as Lupuzor™ to two distinct lupus models. In the lupus-prone B6.lpr mice, we examined neutrophil extracellular traps generation in lupus measured by H3cit expression [ 11 , 19 ]. Our study confirmed that treatments of P140 protected against lupus nephritis with neutrophil infiltration suppression, showing reduced H3cit in neutrophils (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The antibodies used in current study were shown previously, accompanied with the flow cytometry staining protocol [ 19 ]. The other antibodies included CX3CR1 (clone: SA011F11, APC, BioLegend), CD68 (clone: FA-11, FITC, BioLegend), Ki67 (clone: 16A8, APC, BioLegend), CD3ε (clone: 145-2C11, PerCP/Cy5.5, BD Biosciences), CD4 (clone: RM4-5, FITC, BD Biosciences), MHC-II Ab (clone: AF6-120.1, FITC, BD Biosciences), IL-1R1 (clone: 35F5, PE, BD Biosciences).…”

Section: Methodsmentioning

confidence: 99%

“…For ROS detection in vivo, a chemiluminescence probe L-012 (Catalog No. 5085, Wako, Japan) was used as the previous protocol [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…The murine samples were prepared from both the lung and spleens using the previous protocol of flow cytometry analysis [ 19 , 39 ]. Before the single-cell suspension analysis, the lung fragments dissected from each mouse sample were incubated in PBS for 45 min, together with 2 mg/mL collagenase type IV (CAS No.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Phosphopeptides P140 cause oxidative burst responses of pulmonary macrophages in an imiquimod-induced lupus model

Zhong,

Zheng,

Chen

et al. 2023

Mol Biomed

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Recent studies challenge the dogma that a 21-mer phosphopeptide P140 protects against direct cell damage in the phase-III clinical trial (NCT02504645) for lupus, involving reactive oxygen species (ROS)-dependent release of citrullinated histone H3 (H3cit)-linked neutrophil extracellular traps. An open question is the cellular location of ROS production and H3cit formation in lupus. In this study, we examined the effects of P140 peptides on ROS production and H3cit location in lupus with in vivo and situ fluorescence imaging with subcellular resolution. We developed a mouse model of the B6 strain harbouring a bioluminescent reporter under the control of the Lysozyme M promoter. Based on the imiquimod-induced disease model of B6 mice, we used bioluminescent imaging, flow cytometry analysis, and immunohistology staining to study the effects of P140 peptides in lupus. We found a profound accumulation of CX3CR1-positive macrophages in the lungs of lupus mice after the application of P140, accompanied by lung fibrosis formation. The defined P140-mediated macrophage responses were associated with an increase of H3cit in the cytosol, interleukin-1 receptor type 1 on the extracellular membrane, and intracellular production of ROS. Of interest, the disease of imiquimod-induced lupus was prevented with an antioxidant drug apocynin. This study shows that P140 peptides play a role in aggravated murine lupus in a manner dependent on ROS production and H3cit upregulation through pulmonary macrophages.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…For ROS detection in vivo, a chemiluminescence probe L-012 (Catalog No. 5085, Wako, Japan) was used as the previous protocol [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Phosphopeptides P140 cause oxidative burst responses of pulmonary macrophages in an imiquimod-induced lupus model

Zhong,

Zheng,

Chen

et al. 2023

Mol Biomed

Self Cite

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“…Researchers at Beihang University and the Chinese Academy of Sciences extended the study above with antibodies to evaluate NETosis in lupus, an autoimmune condition that can become fatal to the patient overtime. The authors showed that using MPI and anti-Ly6G-SPIOs, neutrophils infiltrated the lung peritoneum due to a lupus-based inflammatory response [144]. Antibody-MPI can be further extended towards understanding the cancer microenvironment using tracers targeting CD4 and CD8 T cell subtypes and can also be exploited for imaging antibody-based immunotherapies [145].…”

Section: Mpi For Cell Tracking and Immunotherapymentioning

confidence: 99%

Magnetic Particle Imaging in Vascular Imaging, Immunotherapy, Cell Tracking, and Noninvasive Diagnosis

et al. 2023

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Magnetic particle imaging (MPI) is a new tracer-based imaging modality that is useful in diagnosing various pathophysiology related to the vascular system and for sensitive tracking of cytotherapies. MPI uses nonradioactive and easily assimilated nanometer-sized iron oxide particles as tracers. MPI images the nonlinear Langevin behavior of the iron oxide particles and has allowed for the sensitive detection of iron oxide-labeled therapeutic cells in the body. This review will provide an overview of MPI technology, the tracer, and its use in vascular imaging and cytotherapies using molecular targets.

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Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

Petcov,

Straticiuc,

Iancu

et al. 2024

JFB

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Nanoparticle (NP)-based solutions for oncotherapy promise an improved efficiency of the anticancer response, as well as higher comfort for the patient. The current advancements in cancer treatment based on nanotechnology exploit the ability of these systems to pass biological barriers to target the tumor cell, as well as tumor cell organelles. In particular, iron oxide NPs are being clinically employed in oncological management due to this ability. When designing an efficient anti-cancer therapy based on NPs, it is important to know and to modulate the phenomena which take place during the interaction of the NPs with the tumor cells, as well as the normal tissues. In this regard, our review is focused on highlighting different approaches to studying the internalization patterns of iron oxide NPs in simple and complex 2D and 3D in vitro cell models, as well as in living tissues, in order to investigate the functionality of an NP-based treatment.

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Noninvasive imaging of the lung NETosis by anti-Ly6G iron oxide nanoparticles

Cited by 3 publications

References 38 publications

Phosphopeptides P140 cause oxidative burst responses of pulmonary macrophages in an imiquimod-induced lupus model

Phosphopeptides P140 cause oxidative burst responses of pulmonary macrophages in an imiquimod-induced lupus model

Magnetic Particle Imaging in Vascular Imaging, Immunotherapy, Cell Tracking, and Noninvasive Diagnosis

Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures

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