Nonequilibrium Plasma‐Activated Antimicrobial Solutions are Broad‐Spectrum and Retain their Efficacies for Extended Period of Time

Ercan, Utku Kürşat; Wang, Hong; Ji, Hai‐Feng; Fridman, Greg; Brooks, Ari D.; Joshi, Suresh G.

doi:10.1002/ppap.201200104

Cited by 115 publications

(160 citation statements)

References 32 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…This is probably due to the increasing amount of plasma energy (J/cm 2 ) deposition in solution, and the associated chemical changes in solution that underlie. Our earlier observation demonstrated that response of biofilm eradication to plasma-activated fluid is exposure-time-dependent [9]. This trend was also observed during biofilm inhibition studies.…”

Section: Resultssupporting

confidence: 61%

“…Surface disinfectants have a major role in eradication of biofilms in this regard. Plasma can either be applied directly to biofilm bearing surface [8] or biofilm can be treated with plasma-treated fluids to inactivate embedded pathogens [9]. Biofilm curing or treatment has been demonstrated using different plasma systems by many investigators [16]- [21], but not the system, set up, and approach we used.…”

Section: Resultsmentioning

confidence: 99%

“…Nonthermal plasma generator used in these studies is showed in Figure 1; and this in-house built electrode and fluid chamber system is reported earlier by our laboratory [9] [10]. The parameters were set to 31.4 kV, 0.29 W/cm 2 , 1500 Hz pulse frequency, pulse duration 10 microseconds, 2 mm of gap between the surface of fluid and DBD electrode.…”

Section: Plasma Setting and Fluid Treatment Conditionsmentioning

confidence: 99%

“…Plasma-treated fluids behave as an oxidant, and inactivate bacterial cells by mounting intracellular oxidative stress [9]. Depending on the type of solution being treated, the treated fluid generates a variable pool of oxidative and/or nitrosative stress in bacterial cells, and possibly target multiple sites for oxidation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Ercan¹,

Joshi²,

Yost³

et al. 2014

AiM

Self Cite

View full text Add to dashboard Cite

Biofilms act as a reservoir of infection, and periodically release cells in vicinity that are capable of developing new biofilm colonies and disseminate infection. Many chronic bacterial infections are serious that are associated with biofilms and have high morbidity and mortality, partly due to their higher resistance to antimicrobial agents, and partly due to lack of strong biocides which can efficiently treat and inhibit biofilm formation. We recently demonstrated that nonequilibrium non-thermal dielectric-barrier discharge plasma (Plasma) can also be applied to control pathogens via applying treated-liquids, and these liquids acquire broad-spectrum antimicrobial properties. In present studies we demonstrated a range of plasma-activated simple chemical solutions which significantly inhibited biofilm formation by multidrug-resistant bacterial pathogens. Plasma-activated methionine solution exhibited strong inhibitory activity against the biofilms of carbapenem-resistant Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, metallo-β-lactamase (NDM1)-positive Klebsiella pneumoniae, and Enterococcus faecalis, and prevented the formation of biofilms by about 70% as compared to untreated controls in single exposure. In addition to inhibition of biofilm formation, a complete inactivation of biofilm-embedded bacterial cells was observed in less than 30 minute's exposure to candidate plasma-activated methionine solution. These findings suggest that plasma-activated solutions have a potential to prevent biofilm formation, and as biofilm inhibitor.

show abstract

Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: Plasma Setting and Fluid Treatment Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Ercan¹,

Joshi²,

Yost³

et al. 2014

AiM

Self Cite

View full text Add to dashboard Cite

show abstract

“…10) PAL has a good shelf-life with a reported retention of its antibacterial activity from days to weeks 7,15) to even years. 16) Apart from water, plasma-treated aqueous buffer and cell culture media are being investigated for cancer therapy. [17][18][19][20] It is without question that the bacteriostatic activity of PAW and other aqueous plasma-treated solutions is predominantly governed by reactive oxygen and nitrogen species (RONS).…”

Section: Introductionmentioning

confidence: 99%

UV–vis spectroscopy study of plasma-activated water: Dependence of the chemical composition on plasma exposure time and treatment distance

Szili

Ogawa

et al. 2017

Jpn. J. Appl. Phys.

View full text Add to dashboard Cite

Plasma-activated water (PAW) is receiving much attention in biomedical applications because of its reported potent bactericidal properties. Reactive oxygen and nitrogen species (RONS) that are generated in water upon plasma exposure are thought to be the key components in PAW that destroy bacterial and cancer cells. In addition to developing applications for PAW, it is also necessary to better understand the RONS chemistry in PAW in order to tailor PAW to achieve a specific biological response. With this in mind, we previously developed a UV-vis spectroscopy method using an automated curve fitting routine to quantify the changes in H 2 O 2 , NO 2 % , NO 3 % (the major long-lived RONS in PAW), and O 2 concentrations. A major advantage of UV-vis is that it can take multiple measurements during plasma activation. We used the UV-vis procedure to accurately quantify the changes in the concentrations of these RONS and O 2 in PAW. However, we have not yet provided an in-depth commentary of how we perform the curve fitting procedure or its implications. Therefore, in this study, we provide greater detail of how we use the curve fitting routine to derive the RONS and O 2 concentrations in PAW. PAW was generated by treatment with a helium plasma jet. In addition, we employ UV-vis to study how the plasma jet exposure time and treatment distance affect the RONS chemistry and amount of O 2 dissolved in PAW. We show that the plasma jet exposure time principally affects the total RONS concentration, but not the relative ratios of RONS, whereas the treatment distance affects both the total RONS concentration and the relative RONS concentrations.

show abstract

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

Karaman

Ercan

Bakay

et al. 2020

Adv Funct Materials

123

View full text Add to dashboard Cite

The threats posed by the impending "postantibiotic era" have put forward urgent challenges to be overcome by providing new diagnostic and therapeutic regimes for improved diagnosis and treatment of bacterial infections. Antibiotic resistance and incurable bacterial infections are especially important in a society faced with rapid demographic changes. With very few new antibiotics in the drug development pipeline, not being able to match the pace of antimicrobial resistance evolution, developments within other fields such as materials sciences and medical technologies are required to realize innovative antibacterial approaches. This progress report presents recent advances in especially nanotechnology-based approaches and their concomitant use with complementary antibacterial treatments. Synergistically improved antibacterial activity can be reached by considering novel, promising approaches such as photodynamic and photothermal therapy as well as cold atmospheric pressure treatments as complementary strategies to fight against antibacterial resistance. Moreover, this report describes how these novel technologies can be further improved especially by integration of nanomaterials into the currently applied single modal strategies against bacterial infections.

show abstract

Nonequilibrium Plasma‐Activated Antimicrobial Solutions are Broad‐Spectrum and Retain their Efficacies for Extended Period of Time

Cited by 115 publications

References 32 publications

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

UV–vis spectroscopy study of plasma-activated water: Dependence of the chemical composition on plasma exposure time and treatment distance

Evolving Technologies and Strategies for Combating Antibacterial Resistance in the Advent of the Postantibiotic Era

Contact Info

Product

Resources

About