Inhibition of Biofilms by Non-Thermal Plasma Treated Novel Solutions

Abstract

Biofilms act as a reservoir of infection, and periodically release cells in vicinity that are capable of developing new biofilm colonies and disseminate infection. Many chronic bacterial infections are serious that are associated with biofilms and have high morbidity and mortality, partly due to their higher resistance to antimicrobial agents, and partly due to lack of strong biocides which can efficiently treat and inhibit biofilm formation. We recently demonstrated that nonequilibrium non-thermal dielectric-barrier discharge plasma (Plasma) can also be applied to control pathogens via applying treated-liquids, and these liquids acquire broad-spectrum antimicrobial properties. In present studies we demonstrated a range of plasma-activated simple chemical solutions which significantly inhibited biofilm formation by multidrug-resistant bacterial pathogens. Plasma-activated methionine solution exhibited strong inhibitory activity against the biofilms of carbapenem-resistant Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, metallo-β-lactamase (NDM1)-positive Klebsiella pneumoniae, and Enterococcus faecalis, and prevented the formation of biofilms by about 70% as compared to untreated controls in single exposure. In addition to inhibition of biofilm formation, a complete inactivation of biofilm-embedded bacterial cells was observed in less than 30 minute's exposure to candidate plasma-activated methionine solution. These findings suggest that plasma-activated solutions have a potential to prevent biofilm formation, and as biofilm inhibitor.

Assessment the effect of non-thermal plasma on Escherichia coli and Staphylococcus aureus biofilm formtion in vitro

“…Thus the effect of non-thermal plasma against biofilm suggested being advisable in treating of biofilm formation by several species of bacteria in vitro. Effect of non-thermal plasma on different physiological activities of bacterial cells was reported by the previous study [12]. From current study, it can be concluded that the non-thermal plasma can reduce the biofilm and that may open wide range of applications that may be solve the biggest problem in the field of bioscience.…”

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

“…Ercan et al . () demonstrated the inhibitory activity of plasma‐activated methionine solution against the biofilms of carbapenem‐resistant Acinetobacter baumannii , methicillin‐resistant Staph. aureus , metallo‐ β ‐lactamase‐positive Klebsiella pneumoniae and Enterococcus faecalis .…”

“…They concluded that the effluent produced by the device is effective against biofilms, provided that the target is fully immersed in the effluent. Ercan et al (2014) demonstrated the inhibitory activity of plasmaactivated methionine solution against the biofilms of carbapenem-resistant Acinetobacter baumannii, methicillin-resistant Staph. aureus, metallo-b-lactamase-positive Klebsiella pneumoniae and Enterococcus faecalis.…”

“…Biofilms demonstrate unusual resistances to almost all forms of sterilization (Brelles-Marino 2012). Different physical and chemical disinfection methods are widely used to inactivate sessile biofilm forms of microorganisms, with variable efficacies (Ercan et al 2014). Chemical disinfection plays a predominant role in controlling biofilms in vitro.…”

Polymerization of D-Ribose in Dielectric Barrier Discharge Plasma

“…The studies on the applications of discharged plasma are currently very active in the medical, environmental, and agriculture fields [1][2][3][4][5][6][7][8][9][10]. Among various plasma techniques, dielectric barrier discharge (DBD) plasma [11,12] is of special interest due to the safety and selectivity in its effects on biological systems [13,14].…”