Nonenzymatic chemiluminescence detection of circulating tumor cells in blood based on Au@luminol nanoparticles, hybridization chain reaction and magnetic isolation

Cao, Hai-Xia; Liu, Pengfei; Wang, Li; Liu, Zhijun; Ye, Shu-Ying; Liang, Guoxi

doi:10.1016/j.snb.2020.128287

Cited by 30 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HCR occurs when initiator strands are introduced into a solution containing two stable species of DNA hairpins to trigger a cascade of hybridization reaction with nicked double helices, analogous to alternating copolymers . Based on this, DNA hairpin sequences can be designed with specific overhangs, which serve as building blocks, enabling the assembly of multiple aptamers via hybridization reaction. − For example, DNA labeled Ag 2 S quantum dots (QDs) and aptamers were prehybridized with the overhangs of hairpin sequences and then assembled via HCR to fabricate multiple Ag 2 S nanodot-aptamer nanoassemblies, affording efficient capture and detection of CTCs (Figure A) . Apart from the hybridization reaction, streptavidin–biotin interaction has also been utilized to anchor multiplex aptamers to HCR-generated long DNA duplexes.…”

Section: Assembly Strategies Of Multivalent Aptamersmentioning

confidence: 99%

Aptamer-Based Detection of Circulating Targets for Precision Medicine

et al. 2021

View full text Add to dashboard Cite

The past decade has witnessed ongoing progress in precision medicine to improve human health. As an emerging diagnostic technique, liquid biopsy can provide realtime, comprehensive, dynamic physiological and pathological information in a noninvasive manner, opening a new window for precision medicine. Liquid biopsy depends on the sensitive and reliable detection of circulating targets (e.g., cells, extracellular vesicles, proteins, microRNAs) from body fluids, the performance of which is largely governed by recognition ligands. Aptamers are single-stranded functional oligonucleotides, capable of folding into unique tertiary structures to bind to their targets with superior specificity and affinity. Their mature evolution procedure, facile modification, and affinity regulation, as well as versatile structural design and engineering, make aptamers ideal recognition ligands for liquid biopsy. In this review, we present a broad overview of aptamer-based liquid biopsy techniques for precision medicine. We begin with recent advances in aptamer selection, followed by a summary of state-of-the-art strategies for multivalent aptamer assembly and aptamer interface modification. We will further describe aptamer-based micro-/nanoisolation platforms, aptamer-enabled release methods, and aptamer-assisted signal amplification and detection strategies. Finally, we present our perspectives regarding the opportunities and challenges of aptamer-based liquid biopsy for precision medicine.

show abstract

Section: Assembly Strategies Of Multivalent Aptamersmentioning

confidence: 99%

Aptamer-Based Detection of Circulating Targets for Precision Medicine

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Nanocatalysts can increase the luminescence yield, such as AuNPs and carbonbased nanomaterials, due to the increased surface area and high electron density [126]. Cao et al proposed an enzyme-free chemiluminescence strategy for CTCs detection based on Au@luminol NPs, hybridization chain reaction (HCR) and magnetic isolation [127]. As illustrated in Figure 7, AS1411 nucleolin aptamer-modified MNPs were utilized to capture and isolate MCF-7 cells from whole blood samples.…”

Section: Chemiluminescence Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

View full text Add to dashboard Cite

The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

show abstract

“…The principle of electrochemical detection is to modify the electrode using nanomaterials and cancer cell specific aptamer, and CTC is bonded to aptamer (on the electrode surface), which will cause the current/electrochemiluminescence (ECL)/photoelectrochemical (PEC) signal to decrease or EC impedance spectroscopy (EIS) signal to increase [27,[92][93][94][95][96]. By establishing a linear relationship between the change and the number of CTC, the quantification can be realized.…”

Section: Electrochemical Detection Of Ctcmentioning

confidence: 99%

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

View full text Add to dashboard Cite

Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

show abstract

Nonenzymatic chemiluminescence detection of circulating tumor cells in blood based on Au@luminol nanoparticles, hybridization chain reaction and magnetic isolation

Cited by 30 publications

References 38 publications

Aptamer-Based Detection of Circulating Targets for Precision Medicine

Aptamer-Based Detection of Circulating Targets for Precision Medicine

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Contact Info

Product

Resources

About