Nondestructive Quantification of Analyte Diffusion in Cornea and Sclera Using Optical Coherence Tomography

Ghosn, Mohamad G.; Tuchin, Valery V.; Larin, Kirill V.

doi:10.1167/iovs.06-1331

Cited by 87 publications

(71 citation statements)

References 44 publications

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“…6. The average permeability coefficient was computed by analyzing the OCTSS changes induced by glucose diffusion in a specific depth region.…”

Section: Methodsmentioning

confidence: 99%

“…Recently we demonstrated the capability of OCT for noninvasive and nondestructive monitoring and quantifying of the permeability coefficient of different analytes and drugs in various biological tissues. [6][7][8][9] In this letter, we report results on quantification of diffusion of biomolecules in normal and diseased areas of pig aorta. The diagnosis from information provided by OCT could help in timely and effective treatment.…”

Section: Introductionmentioning

confidence: 99%

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Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

et al. 2008

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Abstract. Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography ͑OCT͒ is an ideal diagnostic tool due to the high resolution this technology achieves in discriminating the different features of atherosclerotic lesions based on structural imaging. We explore the capability of OCT for functional imaging of normal and atherosclerotic aortic tissues based on time-and depth-resolved quantification of the permeability of biomolecules through these tissues. The permeability coefficient of 20% aqueous solution of glucose was found to be ͑6.80± 0.18͒ ϫ 10 −6 cm/ s in normal aortas and ͑2.69± 0.42͒ ϫ 10 −5 cm/ s in aortas with atherosclerotic disease. The results suggest that this new OCT functional imaging method-the assessment of the permeability coefficients of various physiologically neutral biomolecules in vascular tissues-could assist in early diagnosing and detecting the different components of atherosclerotic lesions.

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“…6. The average permeability coefficient was computed by analyzing the OCTSS changes induced by glucose diffusion in a specific depth region.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…The OCT signal slope (OCTSS) method, previously described by us in detail ( 12 ), was used to calculate the permeability rate of lipoprotein particles and glucose molecules moving through CEA tissues. As they traveled through the carotid tissue, they caused changes in the optical properties of the sample.…”

Section: Discussionmentioning

confidence: 99%

Permeation of human plasma lipoproteins in human carotid endarterectomy tissues: measurement by optical coherence tomography

Ghosn

Mashiatulla

Syed

et al. 2011

Journal of Lipid Research

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“…37,38 The permeability coefficient is obtained by analyzing the changes of OCTSS in a selected region during the diffusion process of hyperosmotic agents. A linear region with minimal fluctuation in the averaged OCT signal profile is selected and its physical thickness (ΔL) is measured (assuming the refractive index of 1.4).…”

Section: Methodsmentioning

confidence: 99%

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography

Zhu

Wei

et al. 2012

J. Biomed. Opt

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Abstract. Hyperosmotic agents have shown great potential in tissue optical clearing. However, the low efficiency of the permeation in biological tissues seriously restricts its application in reality. The synergy of sonophoresis as a penetration enhancer and hyperosmotic agents, 20% glucose (G) and 20% mannitol (M), in optical clearing has been investigated by analyzing the variation of the attenuation coefficients and the permeability coefficients. In the sonophoresis experiments, ultrasound (US) was applied for 10 min before applying hyperosmotic agents. Along with the administration of hyperosmotic agents, the samples were monitored with optical coherence tomography (OCT) functional imaging for the next 2 h. The attenuation coefficients of each group were obtained from the 2-D OCT images based on Beer's Law. The original attenuation coefficient is 12.38 AE 0.73 cm −1 in normal breast tissue. After 45 min treatment, it changes to be 5.91 AE 0.82 cm −1 and 4.14 AE 0.67 cm −1 for 20% G and 20% G/US, respectively. The attenuation coefficient of breast cancer tissue is 18.17 AE 1.45 cm −1 at the beginning, and it becomes 8.70 AE 0.87 cm −1 for 20% G and 6.80 AE 0.92 cm −1 for 20% G/US after 30 min. Meanwhile, the permeability coefficients of hyperosmotic agents were much enlarged by the treatment of ultrasound in both breast normal tissue and breast cancer tissue. A significant difference in permeability coefficients between health tissue and tumor tissue was also observed in the experiment (p < 0.01).

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Nondestructive Quantification of Analyte Diffusion in Cornea and Sclera Using Optical Coherence Tomography

Abstract: Results suggest that the OCT technique might be a powerful tool for noninvasive diffusion studies of different analytes in ocular tissues. However, additional methods of OCT signal acquisition and processing are required to study the diffusion of agents of small concentrations.

Cited by 87 publications

References 44 publications

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

Permeability of hyperosmotic agent in normal and atherosclerotic vascular tissues

Permeation of human plasma lipoproteins in human carotid endarterectomy tissues: measurement by optical coherence tomography

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography

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