Nonantibody-mediated suppression of delayed hypersensitivity to human γ-globulin in mice

Clark, Connie; Azar, Miguel M.; Gleason, Donald F.

doi:10.1016/0008-8749(76)90367-1

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…It might be argued that increased confinement of antigen during the early, and most critical phase of the reaction when sensitized cell-antigen contact occurs, may also promote localization of subsequent am plification processes (lymphokine release), thereby resulting in DHS lesions of much greater magnitude and duration. This con cept received support from the finding that 10/ig of sHGG induced quite minimal de layed reactions in sensitized mice [4] where as lesions elicited using 4 or 10 ug of parti cle-associated HGG were very intense (table II). We suggest that the data presented lends credence to the notion that particulate anti gens are retained at the site of challenge for a longer period of time than noncationic soluble molecules [6, 7|.…”

Section: Discussionsupporting

confidence: 53%

“…Previous histological examination of the cellular infiltrate induced by footpad chal lenge with sHCJG in sensitized mice docu mented the delayed nature of the resulting lesions [4]. In the present report the lesions induced by challenge with HGG-La in simi larly sensitized, as well as control mice, were characterized with respect to quantity, type, and location of the infiltrating cells.…”

Section: Resultsmentioning

confidence: 91%

“…A modification of the method of McConahey and Dixon |16|, as described in a previous report [4), was used to trace label HGG with Na 125l. To assess the humoral response, by immune elimina tion (IE), 10 ug of 125I-HGG was injected intraperitoncally.…”

Section: Lodination and Immune Eliminationmentioning

confidence: 99%

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Characterization and Quantitation of Murine-Delayed Hypersensitivity Responses Elicited with Particle-Associated Human γ-Globulin

Clark¹,

Jauregui²,

Azar³

et al. 1979

Int Arch Allergy Immunol

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Parameters delineating the utilization of particle-associated human γ-globulin to quantitate murine-delayed hypersensitivity (DHS) responses include: (1) the conditions used to prepare HGG-coated polystyrene latex particles; (2) the marked increase in sensitivity of detection of delayed immune responsiveness using particle-bound HGG; (3) a histologic analysis of the lesions induced by HGG-latex in DHS mice, and (4) the increased retention of particle-associated as opposed to soluble HGG at the site of challenge. The potential utilization of particle-bound antigens as a sensitive method of assessing the role of delayed-type responsiveness in tumor and transplantation immunity is discussed.

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Section: Discussionsupporting

confidence: 53%

Section: Resultsmentioning

confidence: 91%

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Characterization and Quantitation of Murine-Delayed Hypersensitivity Responses Elicited with Particle-Associated Human γ-Globulin

Clark¹,

Jauregui²,

Azar³

et al. 1979

Int Arch Allergy Immunol

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“…These investiga tors, in contrast to Vadas et al [14], report ed maximal DHS to keyhole limpet hemocyanin and human serum albumin to occur from 10 to 15 days after immunization. Re ports from this [1] and other laboratories [13] have presented data in accord with Robinson and Naysmith [11] in that peak DHS is expressed approximately 2 weeks after sensitization with soluble protein anti gens emulsified with complete Freund's ad juvant (CFA).…”

Section: Introductionmentioning

confidence: 49%

Comparison of Murine Delayed Hypersensitivity Reactions Elicited with Particle-Associated Versus Soluble Human γ-Globulin

Clark

Azar

1977

Int Arch Allergy Immunol

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Footpad reactions elicited in DHS mice using human γ-globulin (HGG)-coated polystyrene latex particles and soluble HGG (sHGG) were compared. Both the magnitude and the persistence of DHS lesions produced by HGG-latex were considerably greater; at 24, 48 and 72 h after challenge, the level of reactivity induced by HGG-latex was 24, 41 and 71% higher, respectively, than those elicited with sHGG. Early nonspecific swelling following injection of HGG-latex in footpads of normal mice was negligible by 24 h, whereas Arthus-responsive animals did not return to control levels until 48 h. The possible advantages and limitations of using particle-associated protein to elicit DHS reactions are discussed.

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“…SJL mice develop delayed hypersensitiv ity more vigorously than A/J mice [Clark et al, 1976;Crowle et al, 1977], and complete Freund's adjuvant was being used. So, with these extenuating conditions entered into the interpretation, the results, using an antigen not found in mouse chow, essentially agree with ours.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aging in A/J Mice on Delayed and Arthus Hypersensitivities, Anamnesis and a Low-Dose Tolerance

Crowle¹,

Miller²,

Grove³

1981

Gerontology

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This paper describes a 2-year experiment on the flux with aging in A/Jax female mice of Arthus and tuberculin-type delayed hypersensitivities to chicken conalbumin antigen, of delayed hypersensitivity to methylated human serum albumin antigen, of anamnestic responses for these phenomena, and of a low-dose tolerance. The mice began to lose ability to develop delayed hypersensitivity at 15–18 months of age, or 0.6–0.7 mean life span. They retained ability to develop Arthus hypersensitivity, general anamnestic responsiveness, a capacity to develop low-dose tolerance preventing primary induction of delayed hypersensitivity and, once sensitized, the ability to express delayed hypersensitivity reactions well into senescence ( > 0.8 mean life span). These findings agree generally with most others on age-related flux of immunologic responses in mice and man, that cell-mediated responses are shorter-lived than humoral responses, and that induction (primary responsiveness) is much more affected than reaction or anamnesis (secondary responsiveness). Retention with aging of a capacity to develop tolerance, apparently not previously studied, could be important in explaining flagging primary responsiveness, because this (i.e. induction) is what such tolerance suppresses. Thus, certain unanticipated aspects of the experiment reported here suggest that accumulated exposure to food antigens may have contributed to age-related (i.e. time-related) decline of immunologic responses by progressive induction of such tolerance. The simple experimental model described here should be useful for investigating this possibility and the interplay of positive and negative immunologic responses with particular relevance to understanding such effects in aging human beings, since it studies natural changes in whole mice but with greater control, speed and dissectability that could ever be possible in man.

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Nonantibody-mediated suppression of delayed hypersensitivity to human γ-globulin in mice

Cited by 11 publications

References 17 publications

Characterization and Quantitation of Murine-Delayed Hypersensitivity Responses Elicited with Particle-Associated Human γ-Globulin

Characterization and Quantitation of Murine-Delayed Hypersensitivity Responses Elicited with Particle-Associated Human γ-Globulin

Comparison of Murine Delayed Hypersensitivity Reactions Elicited with Particle-Associated Versus Soluble Human γ-Globulin

Effects of Aging in A/J Mice on Delayed and Arthus Hypersensitivities, Anamnesis and a Low-Dose Tolerance

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