Characterization and Quantitation of Murine-Delayed Hypersensitivity Responses Elicited with Particle-Associated Human γ-Globulin

Abstract

Parameters delineating the utilization of particle-associated human γ-globulin to quantitate murine-delayed hypersensitivity (DHS) responses include: (1) the conditions used to prepare HGG-coated polystyrene latex particles; (2) the marked increase in sensitivity of detection of delayed immune responsiveness using particle-bound HGG; (3) a histologic analysis of the lesions induced by HGG-latex in DHS mice, and (4) the increased retention of particle-associated as opposed to soluble HGG at the site of challenge. The potential utilization of particle-bound antigens as a sensitive method of assessing the role of delayed-type responsiveness in tumor and transplantation immunity is discussed.

Delayed-Type Hypersensitivity as Revealed on the Footpads of Mice to Azobenzenearsonate-Acetyl Bovine Serum Albumin

“…For the screening of compounds of potential use in rheumatoid arthritis, these immunological models of inflammation should be reproducible, easy to quantitate and should not be time-consuming. Footpad reaction [4], ear swelling reaction [5], flank skin reaction [6], and percutaneous skin re action [7] have well been established for the induction of DTH in mice [8,9], rats [10,11] or guinea pigs [12,13]. There are varying test antigens: bovine serum albumin [14], ovalbumin [15], lysozyme [16], meth ylated human serum albumin [17], sheep red blood cells [18], and so forth [19].…”