Non-viral gene transfer of murine spleen cells achieved by in vivo electroporation

The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice

“…29,30 The spleen has a central function in the immune system and is highly sensitive to damage by xenobiotics. 21,31 Lymphocyte proliferation is an important phase in the immune response.…”

Nanosized bioceramic particles could function as efficient gene delivery vehicles with target specificity for the spleen

“…The spleen has been documented to be one of the most important lymphoid organs involved in the initiation of immune responses. 19,27 The involvement of its marginal zone through specific macrophage and marginal-zone B cells population can efficiently mount both innate and adaptive immune. PS-SiO 2 -DNA nanoplexes induced potent tumor-specific immune responses following priming mice with PS-SiO 2 -pNGVL-hFLex-MUC-1 nanoplexes.…”

Spleen-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Spleen Surface in Mice

“…[39][40][41] Kasinrerk et al compared intramuscular, intraperitoneal, intravenous, and intrasplenic immunization with a single dose of naked pDNA and observed that only the intrasplenic route induced specific antibody production. 41) Moreover, electroporation combined with intrasplenic administration of naked pDNA enhanced transgene expression level, 12,13) and thus it is expected to improve DNA vaccine efficiency. However, physical force due to direct injection and/or electroporation is harmful to the organ.…”

“…and nuclease in blood. 9) Although it was previously reported that organselective gene transfection using naked pDNA had been achieved by direct injection, electroporation and so on, [10][11][12][13] there is great concern about safety because physical force against the organs is required; consequently, the continuous administration of pDNA is limited.…”