Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways

Schmidt, Anke; Dietrich, Stephan; Steuer, A.; Weltmann, Klaus‐Dieter; Woedtke, Thomas von; Masur, Kai; Wende, Kristian

doi:10.1074/jbc.m114.603555

Cited by 124 publications

(106 citation statements)

References 87 publications

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“…For microscopic evaluation, cells were seeded in 6-well plates and grown to 80 % confluence in DMEM prior to indirect plasma treatment. Twenty-four hours after exposure to plasma treated medium, apoptotic cells were stained using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-technique as previously described [13].…”

Section: Metabolic Activity and Microscopic Evaluationmentioning

confidence: 99%

“…In human keratinocytes we have previously demonstrated a cold plasma-mediated modulation of the metabolic activity of cells, and an alteration of their antioxidant defense [12]. Plasma also triggered hormesis-like processes trough the Nrf2 pathway and thus prepared skin cells against exogenic noxae by increasing their resilience against oxidative species [13].…”

Section: Introductionmentioning

confidence: 98%

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Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Schmidt

Bekeschus

Woedtke

et al. 2015

Clinical Plasma Medicine

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Section: Metabolic Activity and Microscopic Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Schmidt

Bekeschus

Woedtke

et al. 2015

Clinical Plasma Medicine

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“…NTAPP has also been reported to accelerate wound healing processes by activating the nuclear factor erythroid-related factor 2 (NRF2) signaling pathway in human keratinocyte HaCa T cell line in vitro 19, and to promote re-epithelialization and wound closure by activating keratinocytes and fibroblasts in Wistar rats’ wound skin20. These studies strongly suggested that NTAPP stimulates the proliferation of normal and adult stem cells.…”

mentioning

confidence: 97%

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Park

Lee

et al. 2016

Sci Rep

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Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.

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“…Some reports indicate that NAP can enhance the proliferation rate of basal keratinocytes and endothelial cells [75,76] . The 350 downregulated and 400 upregulated transcripts of keratinocytes after NAP treatment highlighted its powerful ability to influence gene expression [77] .…”

Section: Hydrogen Peroxide: Implications For Chronic Wound Treatmentmentioning

confidence: 99%

Hydrogen Peroxide: A Potential Wound Therapeutic Target

Zhu¹,

Wang²,

Lu³

et al. 2017

Med Princ Pract

109

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Hydrogen peroxide (H2O2) is a topical antiseptic used in wound cleaning which kills pathogens through oxidation burst and local oxygen production. H2O2 has been reported to be a reactive biochemical molecule synthesized by various cells that influences biological behavior through multiple mechanisms: alterations of membrane potential, generation of new molecules, and changing intracellular redox balance, which results in activation or inactivation of different signaling transduction pathways. Contrary to the traditional viewpoint that H2O2 probably impairs tissue through its high oxidative property, a proper level of H2O2 is considered an important requirement for normal wound healing. Although the present clinical use of H2O2 is still limited to the elimination of microbial contamination and sometimes hemostasis, better understanding towards the sterilization ability and cell behavior regulatory function of H2O2 within wounds will enhance the potential to exogenously augment and manipulate healing.

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Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways

Cited by 124 publications

References 87 publications

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Hydrogen Peroxide: A Potential Wound Therapeutic Target

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