Non-thermal atmospheric-pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes

Abstract

there was a significant difference in the number of CD83 + cells between triamcinolone-treated and pimecrolimus-treated skin (P = 0.00090) (Fig. 1, Panel 3). Discussion Triamcinolone but not pimecrolimus significantly decreased the number of CD1a + LC in the presence of UV. UV alone did not impact LC expression of CD1a. Studies support the immunosup-pressive effects of triamcinolone on LC (17), which include depletion and alteration of their morphology and function (18). In our study, UV did not decrease CD1a + LC without prior treatment with triamcinolone, and only marginally decreased CD83 expression , implying a combination effect needed for significant LC depletion. Triamcinolone treatment of irradiated skin marginally depleted HLA-DR + LC. While UV did not deplete HLA-DR expression compared with unirradiated skin, pimecrolimus caused higher HLA-DR LC expression in irradiated skin. Pimecrolimus prevented UV-induced depletion of both CD1a and CD83 expression, implying preservation of LC and their maturation. Though pimecrolimus may hinder immune barrier function of the skin (19), only limited case reports and lay articles support its carcinogenic potential (20,21). Pimecrolimus has little effect on dendritic cell maturation, in contrast to corticosteroids (9,10,22). It does not accelerate UV carcinogenesis in mice (23). In subjects with atopic dermatitis, pimecrolimus showed minimal effect on LC (24). Our study examined the effect of pimecrolimus on LC in UV-irradiated human skin for the first time. Because biopsies were performed 2 days after UV, it is possible that some of the LC had migrated from the epidermis. Additionally, LC-mediated immuno-surveillance is just one pathway against tumorigenesis. However, our results support the growing body of evidence that topical cal-cineurin inhibitors do not cause severe immunosuppression. Other experts have made similar conclusions (22,25-27). In the treatment of eczema, topical calcineurin inhibitors remain crucial as an alternative to corticosteroids. Abstract: Non-thermal atmospheric-pressure plasmas provide new hope for improvement in chronic wound management because of their potency in killing microorganisms. However, the effectiveness of the procedure has to be verified and negative effects on healthy tissues have to be excluded. In wound healing adhesion molecules play a crucial role for cell migration and proliferation. We investigated whether an atmospheric-pressure plasma jet (kINPen09) influences the expression of adhesion molecules responsible for cell-cell and cell-matrix interactions after treatment of HaCaT-keratinocytes for 10 and 30 s. Twenty-four hours after plasma treatment expression of a 2-and b 1-integrin, E-cadherin and the epidermal growth factor receptor (EGFR) was determined by flow cytometry. Plasma-treated HaCaT-cells were characterized by normal a 2-integrin and increased b 1-integrin expression. E-cadherin and EGFR expression was reduced after the 30-s treatment. We did not observe any effects following the 10-s plasma treatment. In co...

Skin renewal activity of non-thermal plasma through the activation of β-catenin in keratinocytes

“…Interestingly, Haertel et al . also found that NTP treatment on HaCaT cell reduced the E-cadherin positive cells by FACS analysis 29 . However, they treated NTP on the cells in suspension, and the detection of E-cadherin was performed at 24 hours after the NTP treatment.…”

Helium Generated Cold Plasma Finely Regulates Activation of Human Fibroblast-Like Primary Cells

“…Indeed, a growing body of studies published by Lindequist U. and co-workers has clearly demonstrated that in the non-tumorigenic human keratinocyte cell line HaCaT plasma-generated ROS influences the expression of the surface pattern receptors (e.g. integrins) mainly involved in cellular protrusion [10], [17], [50], [51]. It is well known, nevertheless, that plasma components (such as radiations, ionized nitrogen or ROS), plasma power, the distance from the sample and exposure time affect cells differently.…”

“…This is why some concerns have been expressed with regard to employing plasma in in vivo medical treatments and in wound healing. For example, non-thermal atmospheric pressure plasma affects the expression of adhesion molecules on cellular surfaces causing loss of cell-cell interaction and cellular detachment in HaCaT-keratinocytes [17]. Despite a large body of scientific evidence that has been collected with regard to immortalized cell lines, few studies have been performed until now to investigate the effects of plasmas on cell adhesion and proliferation of primary fibroblasts [18], the main cell population contributing to the healing process.…”

Differential sensitivity of lymphocyte subpopulations to non-thermal atmospheric-pressure plasma

“…Detailed investigations of plasma-cell interactions are needed for each of these different applications. It is known that adhesion molecules can be affected by plasma treatment (Haertel et al 2011) which is important for the wound healing process. However, the extent of down or up regulation of those molecules depends on the plasma source and the treatment time.…”