Non-thermal argon plasma is bactericidal for the intracellular bacterial pathogen Chlamydia trachomatis

Sа, Ermolaeva; Sysolyatina, Elena V.; Колкова, Н И; Bortsov, Petr A; Tuhvatulin, Amir I.; Vasiliev, M. M.; Mukhachev, Andrey; Петров, О. Ф.; Shimizu, Tetsuji; Naroditsky, Boris S.; Morfill, Gregor; Фортов, В. Е.; Grigoriev, Anatoly I.; Zigangirova, Nelly A.; Gintsburg, A. L.

doi:10.1099/jmm.0.038117-0

Cited by 30 publications

(15 citation statements)

References 36 publications

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“…Ermolaeva et al . [32] investigated the effect of non-thermal plasma (NTP) on C. trachomatis -infected McCoy cells. NTP is «the flow of partially ionized, neutral gas obtained at atmospheric pressure» with a temperature of 30–40°C.…”

Section: Discussionmentioning

confidence: 99%

Water-Filtered Infrared A Irradiation in Combination with Visible Light Inhibits Acute Chlamydial Infection

et al. 2014

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New therapeutic strategies are needed to overcome drawbacks in treatment of infections with intracellular bacteria. Chlamydiaceae are Gram-negative bacteria implicated in acute and chronic diseases such as abortion in animals and trachoma in humans. Water-filtered infrared A (wIRA) is short wavelength infrared radiation with a spectrum ranging from 780 to 1400 nm. In clinical settings, wIRA alone and in combination with visible light (VIS) has proven its efficacy in acute and chronic wound healing processes. This is the first study to demonstrate that wIRA irradiation combined with VIS (wIRA/VIS) diminishes recovery of infectious elementary bodies (EBs) of both intra- and extracellular Chlamydia (C.) in two different cell lines (Vero, HeLa) regardless of the chlamydial strain (C. pecorum, C. trachomatis serovar E) as shown by indirect immunofluorescence and titration by subpassage. Moreover, a single exposure to wIRA/VIS at 40 hours post infection (hpi) led to a significant reduction of C. pecorum inclusion frequency in Vero cells and C. trachomatis in HeLa cells, respectively. A triple dose of irradiation (24, 36, 40 hpi) during the course of C. trachomatis infection further reduced chlamydial inclusion frequency in HeLa cells without inducing the chlamydial persistence/stress response, as ascertained by electron microscopy. Irradiation of host cells (HeLa, Vero) neither affected cell viability nor induced any molecular markers of cytotoxicity as investigated by Alamar blue assay and Western blot analysis. Chlamydial infection, irradiation, and the combination of both showed a similar release pattern of a subset of pro-inflammatory cytokines (MIF/GIF, Serpin E1, RANTES, IL-6, IL-8) and chemokines (IL-16, IP-10, ENA-78, MIG, MIP-1α/β) from host cells. Initial investigation into the mechanism indicated possible thermal effects on Chlamydia due to irradiation. In summary, we demonstrate a non-chemical reduction of chlamydial infection using the combination of water-filtered infrared A and visible light.

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Section: Discussionmentioning

confidence: 99%

Water-Filtered Infrared A Irradiation in Combination with Visible Light Inhibits Acute Chlamydial Infection

et al. 2014

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“…The reactive species, charged particles, and UV photons are said to be the major components involved in sterilization of a wide range of gram-positive bacteria, gram-negative bacteria, spores, biofilms, viruses, and fungi [62-64]. …”

Section: Methods Of Productionmentioning

confidence: 99%

Cold Atmospheric Plasma: methods of production and application in dentistry and oncology

2013

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Cold Atmospheric Plasma is an ionized gas that has recently been extensively studied by researchers as a possible therapy in dentistry and oncology. Several different gases can be used to produce Cold Atmospheric Plasma such as Helium, Argon, Nitrogen, Heliox, and air. There are many methods of production by which cold atmospheric plasma is created. Each unique method can be used in different biomedical areas. In dentistry, researchers have mostly investigated the antimicrobial effects produced by plasma as a means to remove dental biofilms and eradicate oral pathogens. It has been shown that reactive oxidative species, charged particles, and UV photons play the main role. Cold Atmospheric Plasma has also found a minor, but important role in tooth whitening and composite restoration. Furthermore, it has been demonstrated that Cold Atmospheric Plasma induces apoptosis, necrosis, cell detachment, and senescence by disrupting the S phase of cell replication in tumor cells. This unique finding opens up its potential therapy in oncology.

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“…The novel NTP system tested was able to significantly reduce E. coil in milk by more than a threefold log reduction without significantly affecting pH or color properties (Gurol et al 2012). Some other scholars have also proved that non-thermal plasma had a significant effect in killing food microbes, such as bacterial, Saccharomyces cerevisiae, spore (Perni et al 2008;Ermolaeva et al 2012;Bayliss et al 2013;Liu et al 2013;Ma et al 2013;Baier et al 2013). An original non-thermal process to inactivate dehydrated (Surowsky et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of non-thermal plasma sterilization on volatile components of tomato juice

Lan

2015

Int. J. Environ. Sci. Technol.

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Pasteurization is an important issue to keep from fruit decay due to microbes for fruit preservation. Conventional heat sterilization is based on thermal process. However, the flavor and nutrition components can be changed by heat damage. In this study, an apparatus was designed to produce non-thermal plasma (NTP), which can be applied in fruit sterilization. NTP can be generated through electric discharge in gaseous chamber. A simple NTP reactor may consist of two electrodes with a chamber and connected to a high-voltage power supply. The new technique of NTP was applied to keep fruit fresh. After the tomatoes were treated by NTP, the volatile flavor components were analyzed by GC-MS and were compared to heat treatment and non-treatment. The results showed that the quality of fresh tomato fruit was unaffected by plasma disinfection treatment, and the contents of trans-2-hexenal and n-hexanal were significantly higher than that by heat processes. NTP has less effect on volatile chemical compositions of tomato juice. NTP technology is a promising method for food pasteurization in the future.

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Non-thermal argon plasma is bactericidal for the intracellular bacterial pathogen Chlamydia trachomatis

Cited by 30 publications

References 36 publications

Water-Filtered Infrared A Irradiation in Combination with Visible Light Inhibits Acute Chlamydial Infection

Water-Filtered Infrared A Irradiation in Combination with Visible Light Inhibits Acute Chlamydial Infection

Cold Atmospheric Plasma: methods of production and application in dentistry and oncology

Effects of non-thermal plasma sterilization on volatile components of tomato juice

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