Non-steroidal anti-inflammatory drugs disturb the osmoregulatory, metabolic and cortisol responses associated with seawater exposure in rainbow trout

Gravel, Amélie; Wilson, Jonathan M.; Pedro, Dalila F.N.; Vijayan, Mathilakath M.

doi:10.1016/j.cbpc.2008.11.002

Cited by 25 publications

(10 citation statements)

References 40 publications

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“…Because carprofen shows long t 1/2λz in fish, repeated dosing may result in residual risk and adverse effects. After prolonged exposure (4–14 days) to NSAIDs (diclofenac, salicylate, ibuprofen, mefenamic acid) following bath administration, adverse effects on reproduction, osmoregulation, homeostatic functions, and immunity have been observed in fish [ 55 , 56 , 57 , 58 ]. There is insufficient data regarding the safety of carprofen in fish.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (Oncorhynchus mykiss) Broodstock

et al. 2021

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The aim of this study was to determine the pharmacokinetics of carprofen following intravenous (IV), intramuscular (IM) and oral routes to rainbow trout (Oncorhynchus mykiss) broodstock at temperatures of 10 ± 1.5 °C. In this study, thirty-six healthy rainbow trout broodstock (body weight, 1.45 ± 0.30 kg) were used. The plasma concentrations of carprofen were determined using high-performance liquid chromatography and pharmacokinetic parameters were calculated using non-compartmental analysis. Carprofen was measured up to 192 h for IV route and 240 h for IM, and oral routes in plasma. The elimination half-life (t1/2λz) was 30.66, 46.11, and 41.08 h for IV, IM and oral routes, respectively. Carprofen for the IV route showed the total clearance of 0.02 L/h/kg and volume of distribution at steady state of 0.60 L/kg. For IM and oral routes, the peak plasma concentration (Cmax) was 3.96 and 2.52 μg/mL with the time to reach Cmax of 2 and 4 h, respectively. The bioavailability was 121.89% for IM route and 78.66% for oral route. The favorable pharmacokinetic properties such as the good bioavailability and long t1/2λz for IM and oral route of carprofen suggest the possibility of its effective use for the treatment of various conditions in broodstock.

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Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (Oncorhynchus mykiss) Broodstock

et al. 2021

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“…All steps, including centrifugation, were carried out at 4°C. The enrichment of the membrane fraction was determined as described previously by measuring the activities of Na + /K + -ATPase [13] , 5′-nucleotidase [14] and lactate dehydrogenase [15] . The six-fold higher Na + /K + -ATPase (H: 1.2±0.1 vs. M: 6.9±1.1; n = 7–8) and thirteen-fold higher 5′- nucleotidase (H: 16±1.5 vs. M: 178±48; n = 7–8) activities (U/g protein) in the membrane (M) fraction compared to the initial tissue homogenate (H), confirm membrane enrichment.…”

Section: Methodsmentioning

confidence: 99%

Novel Nongenomic Signaling by Glucocorticoid May Involve Changes to Liver Membrane Order in Rainbow Trout

et al. 2012

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Stress-induced glucocorticoid elevation is a highly conserved response among vertebrates. This facilitates stress adaptation and the mode of action involves activation of the intracellular glucocorticoid receptor leading to the modulation of target gene expression. However, this genomic effect is slow acting and, therefore, a role for glucocorticoid in the rapid response to stress is unclear. Here we show that stress levels of cortisol, the primary glucocorticoid in teleosts, rapidly fluidizes rainbow trout (Oncorhynchus mykiss) liver plasma membranes in vitro. This involved incorporation of the steroid into the lipid domains, as cortisol coupled to a membrane impermeable peptide moiety, did not affect membrane order. Studies confirmed that cortisol, but not sex steroids, increases liver plasma membrane fluidity. Atomic force microscopy revealed cortisol-mediated changes to membrane surface topography and viscoelasticity confirming changes to membrane order. Treating trout hepatocytes with stress levels of cortisol led to the modulation of cell signaling pathways, including the phosphorylation status of putative PKA, PKC and AKT substrate proteins within 10 minutes. The phosphorylation by protein kinases in the presence of cortisol was consistent with that seen with benzyl alcohol, a known membrane fluidizer. Our results suggest that biophysical changes to plasma membrane properties, triggered by stressor-induced glucocorticoid elevation, act as a nonspecific stress response and may rapidly modulate acute stress-signaling pathways.

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“…Regarding the effects of salicylates, Zivna et al (2013) see the reason for increased body weight and growth rate on the release of several important hormones influencing homeostasis in fish, a phenomenon which has been described in many studies (Van Anholt et al, 2003;Gravel and Vijayan, 2007a;Gravel and Vijayan, 2007b;Gravel et al, 2009). It has also been demonstrated that salicylates may regulate plant growth and productivity (Hayat et al, 2010).…”

Section: Discussionmentioning

confidence: 94%

Effect of salicylic acid on early life stages of common carp (Cyprinus carpio)

Zivna¹,

Sehonová²,

Plhalová³

et al. 2015

Environmental Toxicology and Pharmacology

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Non-steroidal anti-inflammatory drugs disturb the osmoregulatory, metabolic and cortisol responses associated with seawater exposure in rainbow trout

Cited by 25 publications

References 40 publications

Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (Oncorhynchus mykiss) Broodstock

Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (Oncorhynchus mykiss) Broodstock

Novel Nongenomic Signaling by Glucocorticoid May Involve Changes to Liver Membrane Order in Rainbow Trout

Effect of salicylic acid on early life stages of common carp (Cyprinus carpio)

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