Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplemented with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degrading enzymes

Morgan, Natalie K.; Bhuiyan, Momenuzzaman; Hopcroft, R.

doi:10.1016/j.psj.2022.101846

Cited by 10 publications

(7 citation statements)

References 39 publications

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“…Some other works on thermotolerant xylanases from Aspergillus sp. BCC125 [26], Aspergillus sulphureus [27], Ruminococcaceae, Prevotella, and Lactobacillu [28] have also reached a relatively high expression level. In our study, we successfully achieved the expression of thermophilic xylanase TmxN3 with enzyme activity that reached the ultra 1.52 × 10 6 U/mL at 100 • C, and the protein content of 6.80 mg/mL in a 5L bioreactor.…”

Section: Xylanase Tmxn3 Exhibited Excellent Thermal Stabilitymentioning

confidence: 99%

Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis

Hu,

Hong,

Zhu

et al. 2024

Molecules

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Xylanase is an essential component used to hydrolyze the xylan in wheat flour to enhance the quality of bread. Presently, cold-activated xylanase is popularly utilized to aid in the development of dough. In this study, ancestral sequence reconstruction and molecular docking of xylanase and wheat xylan were used to enhance the activity and stability of a thermophilic xylanase. The results indicated that the ancestral enzyme TmxN3 exhibited significantly improved activity and thermal stability. The Vmax increased by 2.7 times, and the catalytic efficiency (Kcat/Km) increased by 1.7 times in comparison to TmxB. After being incubated at 100 °C for 120 min, it still retained 87.3% of its activity, and the half-life in 100 °C was 330 min, while the wild type xylanase was only 55 min. This resulted in an improved shelf life of bread, while adding TmxN3 considerably enhanced its quality with excellent volume and reduced hardness, chewiness, and gumminess. The results showed that the hardness was reduced by 55.2%, the chewiness was reduced by 40.11%, and the gumminess was reduced by 53.52%. To facilitate its industrial application, we further optimized the production conditions in a 5L bioreactor, and the xylanase activity reached 1.52 × 106 U/mL culture.

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Section: Xylanase Tmxn3 Exhibited Excellent Thermal Stabilitymentioning

confidence: 99%

Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis

Hu,

Hong,

Zhu

et al. 2024

Molecules

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“…All diets contained 0.5% titanium dioxide (TiO 2 ) as a digestibility marker and supplemental phytase at commercial levels (Quantum Blue, AB Vista). Soluble and insoluble NSP and free oligosaccharides were measured in the dietary treatments using the procedure described in Morgan et al (2022). Birds had ad libitum access to water and feed throughout the trial period.…”

Section: Dietary Treatmentsmentioning

confidence: 99%

Comparing a single dose of xylanase to a double dose or cocktail of non-starch polysaccharide-degrading enzymes in broiler chicken diets

Morgan

Bhuiyan

Wallace

et al. 2022

JAAN

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This study compared supplementation with a single dose of xylanase to a double dose of xylanase or a non-starch polysaccharide (NSP) degrading enzyme cocktail (NSP-ase cocktail) on productive performance, nutrient utilisation and the gastrointestinal environment in broilers fed commercial diets. Cobb 500 broilers (n=1,080) were fed 12 dietary treatments; four Australian commercial diets (based on wheat-barley, wheat-maize, wheat-sorghum or wheat only) with three different enzyme treatments (single dose of xylanase (16,000 BXU/kg), double dose of xylanase (32,000 BXU/kg) or NSP-ase cocktail (xylanase, β-glucanase, cellulase, pectinase, mannanase, galactanase, arabinofuranosidase). There were 108 pens, nine replicates per dietary treatment, with 10 birds per pen. Performance (total pen body weight, feed intake and feed conversion ratio corrected for mortality) was determined at d 0-35. On d 35, one male and one female were weighed individually and used to determine breast meat, thigh and drumstick weight, dry matter (DM) contents from the gizzard, jejunum and ileum, ileal protein, energy, starch and dry matter digestibility, ileal viscosity and xylo-oligosaccharide (XOS) concentration, caecal microbiota and short chain fatty acid (SCFA) composition. The double dose of xylanase and NSP-ase cocktail had no effect on bird performance, meat yield, ileal viscosity, ileal starch, energy or DM digestibility or digesta DM content. The double xylanase dose and NSP-ase cocktail increased protein digestibility in birds fed the wheat-sorghum based diet (P=0.041) and increased caecal concentration of butyric acid in birds fed the wheat-maize based diet (P=0.040), and propionic, valeric and lactic acid and Bifidobacteria and Enterobacteria spp. in birds fed the wheat-based diet (P<0.05). The double xylanase dose increased XOS production, particularly in birds fed the wheat-barley based diets (P<0.05). The lack of performance effects observed when feeding the double xylanase dose or NSP-ase cocktail suggested that the current recommended xylanase dose (16,000 BXU/kg) is sufficient.

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“…Among the exogenous enzymes, xylanase is normally used to improve non‐starch polysaccharide (NSP) degradation of diets in broilers (Morgan et al., 2022 ). The improvement in digestibility and the enhancement in nutrient utilization are important for the improved growth performance (Varkoohi et al., 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of phytase and xylanase enzymes on growth performance and Mucin2 gene expression in broiler chickens

Noormohammadi

Varkoohi

Seyedabadi

2023

Veterinary Medicine & Sci

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Background: Development of exogenous enzymes is one of the most important discoveries in animal nutrition. The supplementation of exogenous enzymes in broiler diets allows for supplying nutrient deficiencies and to decrease endogenous losses. Objectives:The effects of phytase (Hostazym and Phyzyme) and xylanase (Ronozyme) enzymes were investigated on growth performance and Mucin2 gene expression in broilers.Methods: A completely randomized design was applied, including 7 treatments, 4 replicates and 25 birds per replicates. A total of 700 male Ross (308) broiler chickens were fed with similar diets supplemented by Hostazym and Phyzyme (500 and 1000 FTU/kg) and Ronozyme (100 and 200 EXU/kg). Weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were determined for three phases and entire rearing period. On 42 days of age, four birds per replicate were slaughtered. Total RNA was extracted from jejunum samples, and Mucin2 gene expression was measured by real-time PCR.Results: Phytase and xylanase enzymes had a significant effect (p < 0.05) on traits (WG and FCR) in grower and finisher phases and whole rearing period, but FI was not affected by enzymes (p > 0.05). Carcass (74.13 g) and breast (27.76 g) weights by Hostazym (1000 FTU/kg) were higher than other treatments (p < 0.05). Weight of liver, bursa and spleen were significantly influenced by enzymes (p < 0.05). Likewise, bursa and spleen weights in Hostazym (1000 FTU/kg feed) and Ronozyme (200 EXU/kg feed) were significantly higher than other treatments (p < 0.05). Mucin2 gene expression was affected by enzymes in whole treatments. The lowest amount of Mucin2 gene expression belonged to Ronozyme (200 and 100 EXU/kg), and the highest was belonging to Hostazym (1000 FTU/kg). Conclusions: Phytase enzymes have higher effect on broiler performance and Mucin2gene expression compared to xylanase. High doses of Hostazym (1000 FTU/kg feed) could be supplemented in broiler chicken diets to improve optimum growth and feed efficiency.

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Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplemented with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degrading enzymes

Cited by 10 publications

References 39 publications

Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis

Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis

Comparing a single dose of xylanase to a double dose or cocktail of non-starch polysaccharide-degrading enzymes in broiler chicken diets

Effect of phytase and xylanase enzymes on growth performance and Mucin2 gene expression in broiler chickens

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