Effect of phytase and xylanase enzymes on growth performance and Mucin2 gene expression in broiler chickens

Abstract

Background: Development of exogenous enzymes is one of the most important discoveries in animal nutrition. The supplementation of exogenous enzymes in broiler diets allows for supplying nutrient deficiencies and to decrease endogenous losses. Objectives:The effects of phytase (Hostazym and Phyzyme) and xylanase (Ronozyme) enzymes were investigated on growth performance and Mucin2 gene expression in broilers.Methods: A completely randomized design was applied, including 7 treatments, 4 replicates and 25 birds per replicates. A total of 700 male Ross (308) broiler chickens were fed with similar diets supplemented by Hostazym and Phyzyme (500 and 1000 FTU/kg) and Ronozyme (100 and 200 EXU/kg). Weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were determined for three phases and entire rearing period. On 42 days of age, four birds per replicate were slaughtered. Total RNA was extracted from jejunum samples, and Mucin2 gene expression was measured by real-time PCR.Results: Phytase and xylanase enzymes had a significant effect (p < 0.05) on traits (WG and FCR) in grower and finisher phases and whole rearing period, but FI was not affected by enzymes (p > 0.05). Carcass (74.13 g) and breast (27.76 g) weights by Hostazym (1000 FTU/kg) were higher than other treatments (p < 0.05). Weight of liver, bursa and spleen were significantly influenced by enzymes (p < 0.05). Likewise, bursa and spleen weights in Hostazym (1000 FTU/kg feed) and Ronozyme (200 EXU/kg feed) were significantly higher than other treatments (p < 0.05). Mucin2 gene expression was affected by enzymes in whole treatments. The lowest amount of Mucin2 gene expression belonged to Ronozyme (200 and 100 EXU/kg), and the highest was belonging to Hostazym (1000 FTU/kg). Conclusions: Phytase enzymes have higher effect on broiler performance and Mucin2gene expression compared to xylanase. High doses of Hostazym (1000 FTU/kg feed) could be supplemented in broiler chicken diets to improve optimum growth and feed efficiency.

Effect of an Escherichia coli–derived phytase and a carbohydrase–protease cocktail derived from Bacillus spp. on performance, digestibility, bone mineralization and gut morphology in broilers fed different nutrient density diets

“…The findings from investigations on NSP‐degrading enzymes in broiler chickens are less conclusive than those from studies involving PHY. In some trials, no or just minimal impacts were detected (Karimi et al., 2013 ; Noormohammadi et al., 2023 ; Tiwari et al., 2010 ), but in others, greater nutrient digestibility and growth rate were discovered (Cozannet et al., 2019 ; Kiarie et al., 2021 ). It is difficult to compare the effects of NSP‐degrading enzymes across studies because of the range of types and doses of enzymes employed separately or in combination.…”