Non-Solvatochromic Cell Membrane-Targeted NIR Fluorescent Probe for Visualization of Polarity Abnormality in Drug-Induced Liver Injury Mice

Abstract

Noninvasive visualization of liver polarity by using fluorescence imaging technology is helpful to better understand drug-induced liver injury (DILI). However, cell membrane-targeted polarity-sensitive near-infrared (NIR) fluorescent probes are still scarce. Herein, we report a non-solvatochromic cell membrane-targeted NIR small molecular probe (N-BPM-C 10 ) for monitoring the polarity changes on cell membranes in living cells and in vivo. N-BPM-C 10 exhibits polarity-dependent fluorescence around 655 nm without an obvious solvatochromic effect, which endows it with good capability for the in vivo imaging study. Moreover, it can rapidly and selectively light up the cell membranes as well as distinguish tumor cells from normal cells due to its excellent polarity-sensitive ability. More importantly, N-BPM-C 10 has been successfully applied to visualize liver polarity changes in vivo, revealing the reduction of liver polarity in DILI mice. We believe that N-BPM-C 10 provides a new way for the diagnosis of DILI.

A ratiometric fluorescent probe for imaging the fluctuation of HOBr during endoplasmic reticulum stress

“…Usually, fluorescent probes are used as powerful detection tools due to their outstanding sensitivity, low cytotoxicity, and super spatial and temporal resolution. 9–11 Though HOBr shares similar properties with HOCl, its concentration in vivo is significantly lower than that of HOCl. 12,13 As a result, there are limited fluorescent probes available for detecting HOBr compared to those for HOCl.…”

Tracking Plasma Membrane Damage Using a Ruthenium(II) Complex Phosphorescent Indicator Paired with Cholesterol

“…Electron microscopy, transmission electron microscopy techniques, and nuclear magnetic resonance (NMR) spectroscopy have been employed to investigate membrane penetration, − but these techniques are not capable of real-time imaging plasma membrane. The fluorescence imaging technique is a useful tool for facilely monitoring the plasma membrane behavior because of its high sensitivity, selectivity, and capacity for real-time in situ detection. − Although the plasma membrane indicators using organic dyes as the fluorescence cores have the advantages of high fluorescence quantum yields, large molar extinction coefficients, and adjustable emission wavelengths, their some performances still need to be improved including the following: (1) poor photostability and easy to be photobleached, (2) small Stokes shifts and easy to be interfered by excitation light, and (3) short fluorescence lifetimes and easy to be interfered by self-luminescence biological backgrounds and scattered lights of nearby optical systems . In contrast, transition metal complexes as a kind of excellent luminophores have been widely used in the field of luminescence probes due to their unique properties and versatility. − In our previous studies, we have successfully developed some luminescent probes based on Ru­(II)/Ir­(III) complexes, − which exhibited excellent features including good biocompatibility, larger Stokes shifts, high photophysicochemical stabilities, and long luminescence lifetimes.…”

Fast-Responsive HClO-Activated Near-Infrared Fluorescent Probe for In Vivo Diagnosis of Inflammatory Bowel Disease and Ex Vivo Optical Fecal Analysis

“…However, X-ray examination carries the risk of exposure to ionizing radiation, endoscopy is invasive, and histological examination has the potential to cause secondary trauma. , In addition, stool testing shows poor specificity and can hardly provide real-time feedback for diagnosis . Compared to traditional methods, fluorescence imaging has attracted great attention in IBD detection in recent years due to its advantages in high sensitivity, noninvasiveness, superior spatiotemporal resolution, and real-time visualization. − Therefore, the development of highly sensitive fluorescent probes holds great significance in the diagnosis and treatment of IBD.…”