Non small cell carcinoma of lung with metachronous breast metastasis and cardiac tamponade: Unusual presentation of a common cancer

Venkatesulu, Bhanu Prasad; Mallick, Supriya; Singh, Ashok; Julka, Pramod Kumar

doi:10.1016/j.jnci.2015.03.006

Cited by 5 publications

(4 citation statements)

References 9 publications

(6 reference statements)

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“…NSCLC comprises lung squamous cell carcinoma (LUSC), lung adenocarcinoma (LUAD) and large cell lung cancer (LCLC), and represents 85% of all LC cases [5][6][7][8]. Despite recent diagnostic and therapeutic innovations, the overall 5-year survival rate of patients with NSCLC is only 10% [9], which prompted us to explore the molecular mechanism underlying the initiation and development of NSCLC and to identify novel targets for enhancing the diagnosis and treatment of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

et al. 2018

Cell Physiol Biochem

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Background/Aims: The purpose of this study was to probe the clinico-pathological significance and the underlying mechanism of miR-30d-5p expression in non-small cell lung cancer (NSCLC). Methods: We initially examined the level of miR-30d-5p expression in NSCLC and non-cancer tissues using RT-qPCR. Then, a series of validation analyses including a meta-analysis of data from microarray chips in Gene Expression Omnibus (GEO), data mining of the cancer genome atlas (TCGA) and an integrated meta-analysis incorporating GEO microarray chips, TCGA data, in-house RT-qPCR and literature studies were performed to examine the clinico-pathological value of miR-30d-5p expression in NSCLC. In vitro experiments were further conducted to investigate the impact of miR-30d-5p on NSCLC cell growth. The molecular mechanism by which miR-30d-5p regulates the pathogenesis of NSCLC was probed through a bioinformatics analysis of its target genes. Moreover, dual luciferase reporter assay was conducted to verify the targeting regulatory relationship between miR-30d-5p and CCNE2. Results: Based on results from RT-qPCR, GEO meta-analysis, TCGA data mining and the integrated meta-analysis incorporating GEO microarray chips, TCGA data, in-house RT-qPCR and literature studies, miR-30d-5p expression was decreased in NSCLC tissues, and patients with NSCLC who presented with lower miR-30d-5p expression tended to display an advanced clinical progression. Significant pathways including the Mucin type O-glycan biosynthesis pathway, cell cycle pathway and cysteine and methionine metabolism pathway (all P< 0.05) revealed potential roles of the target genes of miR-30d-5p in the oncogenesis of NSCLC. Results from in vitro experiments indicated that miR-30d-5p could attenuate proliferation and viability of NSCLC cells. Among the 12 identified hub genes, nine genes including E2F3, CCNE2, SKP2, CDK6, TFDP1, LDHA, GOT2, DNMT3B and ST6GALNAC1 were validated by Pearson’s correlation test and the human protein atlas (HPA) database as targets of miR-30d-5p with higher probability. Specifically, dual luciferase reporter assay confirmed that CCNE2 was directly targeted by miR-30d-5p. Conclusion: In summary, miR-30d-5p expression is decreased in NSCLC, and it might play the role as tumor suppressor in NSCLC by regulating target genes.

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Section: Introductionmentioning

confidence: 99%

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

et al. 2018

Cell Physiol Biochem

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“…Between 75 and 80% of lung cancer cases are non-small cell lung cancer (NSCLC), which has an overall 5-year survival rate of only 10% (2). Despite novel methods targeting early diagnosis and recent advancements in treatments, the prognosis and survival rate of NSCLC patients remains relatively poor.…”

Section: Introductionmentioning

confidence: 99%

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

Tian

Yue

et al. 2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs/miRs) are a class of small, highly conserved non-coding RNAs that can serve either oncogenic or tumor-suppressive roles in a wide variety of tumors. miR-200c is a member of the miR-200 family whose specific role in non-small cell lung cancer (NSCLC) has not yet been elucidated. The purpose of the present study was to detect the expression level of miR-200c in NSCLC, and to analyze its association with clinicopathological factors and patient prognosis. The present study determined the expression levels of miR-200c in 110 tumor samples collected from patients diagnosed with NSCLC who underwent complete tumor resection with regional lymph node dissection, as assessed by reverse transcription-quantitative polymerase chain reaction. The association between the expression level of miR-200c and clinicopathological features and patient prognosis was also analyzed. The results showed that miR-200c overexpression was detected in 66 of the 110 cases and was significantly associated with positive lymph node metastasis (P<0.001). Univariate survival analysis demonstrated that high miR-200c expression, positive lymph node metastasis and advanced Tumor-Node-Metastasis (TNM) classification stage significantly predicted decreased 5-year disease-free survival rates (all P<0.05) and poor 5-year overall survival rates (all P<0.01), respectively. The results of multivariate Cox regression analysis showed that TNM stage and miR-200c expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival rates (P<0.05) and poor 5-year overall survival rates (P<0.01). The present findings suggest that miR-200c overexpression is significantly associated with poor survival rates in NSCLC and that miR-200c could play an oncogenic role. miR-200c may have clinical potential as a promising prognostic predictor for patients with NSCLC.

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“…Hence depletion in circulating pool of lymphocytes might lead to suboptimal treatment outcomes. Radiation induced lymphopenia (RIL) has been shown to be an independent predictor of inferior overall survival in gliomas, pancreatic cancer, lung, hepatocellular malignancies and inferior progression free survival (PFS) in head and neck cancers [8,[10][11][12][13][14]. RIL has also been associated with poor response to therapy and higher recurrence rates in cervical and bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

IL-15 in Pancreatic Cancer

Pature

2018

JPCR

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Non small cell carcinoma of lung with metachronous breast metastasis and cardiac tamponade: Unusual presentation of a common cancer

Abstract: The importance of this case is to highlight molecular profiling as an applicable tool to distinguish extra-mammary metastasis that masquerade as mammary neoplasm thereby preventing unnecessary need of surgery and radiation therapy.

Cited by 5 publications

References 9 publications

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

Potential use of microRNA-200c as a prognostic marker in non-small cell lung cancer

IL-15 in Pancreatic Cancer

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