Non-random chromosome positioning in human sperm and sex chromosome anomalies following intracytoplasmic sperm injection

Luetjens, C. Marc; Payne, Christopher J.; Schatten, Gerald

doi:10.1016/s0140-6736(99)80059-2

Cited by 84 publications

(81 citation statements)

References 4 publications

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“…Among these three chromosomes, CHR 6 had a tendencyto occupythe anterior position. Earlier, preferential localization of CHR X in the sub-acrosomal part of sperm was shown using a similar approach (Luetjens et al 1999,Hazzouri et al 2000. CHR 18 was found in the basal half in 60% of sperm cells (Luetjens et al 1999), while CHR 13 did not demonstrate anyrestricted positioning (Hazzouri et al 2000).…”

Section: Discussionmentioning

confidence: 70%

“…Earlier, preferential localization of CHR X in the sub-acrosomal part of sperm was shown using a similar approach (Luetjens et al 1999,Hazzouri et al 2000. CHR 18 was found in the basal half in 60% of sperm cells (Luetjens et al 1999), while CHR 13 did not demonstrate anyrestricted positioning (Hazzouri et al 2000). It is noteworthythat sex chromosome X was found in a position close to the place of the first contact between sperm and egg, not onlyin humans but also in evolutionary distant marsupial and monotreme mammals (Greaves et al 2003).…”

Section: Discussionmentioning

confidence: 83%

“…More recently, data using fluorescent in situ hybridization (FISH) started to emerge indicating specific chromosome location in human sperm nuclei (Luetjens et al 1999,Hazzouri et al 2000,Sbracia et al 2002. Tilgen and coauthors (2001) studied the arrangement of five chromosome pairs in human spermatozoa and each pair was found to have a non-random spatial distribution.…”

Section: Introductionmentioning

confidence: 99%

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Non-random positioning of chromosomes in human sperm nuclei

2004

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In human spermatozoa, the arrangement of chromosomes is non-random. Characteristic features are association of centromeres in the interior chromocenter and peripheral location of telomeres. In this paper, we have investigated the highest level of order in DNA packing in sperm ^ absolute and relative intranuclear chromosome positioning. Asymmetrical nuclear shape, existence of a defined spatial marker, and the haploid complement of chromosomes facilitated an experimental approach using in situ hybridization. Our results showed the tendencyfor non-random intranuclear location of individual chromosome territories. Moreover, centromeres demonstrated specific intranuclear position, and were located within a limited area of nuclear volume. Additionally, the relative positions of centromeres were non-random; some were found in close proximity, while other pairs showed significantly greater intercentromere distances. Therefore, a unique and specific adherence mayexist between chromosomes in sperm. The observed chromosome order is discussed in relation to sperm nuclei decondensation, and reactivation during fertilization.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

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Non-random positioning of chromosomes in human sperm nuclei

2004

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“…An increased rate of sex chromosomal anomalies has been reported in human babies conceived by ICSI [42]. This increase could be due to the fact that the sex chromosome X was found to be preferentially localized at the subacrosomal region of the sperm [8,10,43]. It may be suggested that these anomalies, at least in part, are connected with delayed chromosome processing of sperm chromosomes residing in the apical tip [43].…”

Section: Discussionmentioning

confidence: 99%

“…This increase could be due to the fact that the sex chromosome X was found to be preferentially localized at the subacrosomal region of the sperm [8,10,43]. It may be suggested that these anomalies, at least in part, are connected with delayed chromosome processing of sperm chromosomes residing in the apical tip [43]. Furthermore, preservation of intact acrosome during ICSI most probably is the major cause of delayed or hindered decondensation of the apical region of the sperm during PN development (see example Fig.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of human male pronuclear development in a heterologous ICSI model

Jones

Mudrak

Zalensky

2010

J Assist Reprod Genet

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Purpose To evaluate human sperm nuclear chromatin decondensation in a heterologous ICSI system using hamster ova injected with human sperm. Materials and methods Frozen hamster oocytes were injected with Triton X-100 treated sperm and fixed at different time points post ICSI. Oocytes injected with nontreated sperm served as controls. Male pronuclear decondensation was evaluated after staining with DAPI. Results Sperm cells with partially destroyed membranes and depletion of the acrosome decondense more rapidly and to a greater extent than membrane/acrosome intact cells. Marked variability in pronuclear size was observed for any time point post ICSI, which most probably reflects the heterogeneity in the mature human sperm population. Conclusion Remodeling of male gamete nuclei in this heterologous ICSI mimics events that occur during natural fertilization in humans and therefore this approach may be used for studies of human sperm chromosomes transformations.

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