Genome organization in the human sperm nucleus studied by FISH and confocal microscopy

Hazzouri, Mira; Rousseaux, Sophie; Mongélard, Fabien; Usson, Yves; Pelletier, R.; Faure, Anne‐Karen; Vourc’h, Claire; Sèle, B

doi:10.1002/(sici)1098-2795(200003)55:3<307::aid-mrd9>3.0.co;2-p

Cited by 86 publications

(95 citation statements)

References 54 publications

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“…In this regard, MNDS labelling resembles that obtained by Differential packaging of mouse sperm chromatin immunocytochemical analysis of mouse sperm using antibodies to H1 (Pittoggi et al 1999), while the MNDI labelling resembles the variant histone-enriched pericentromeric heterochromatin reported by Govin et al (2007) and Van der Heijden et al (2006). Similar structures have been reported in human spermatozoa using centromeric FISH probes (Hazzouri et al 2000) and antibodies to the sperm-specific CENP-A histone variant in bovine spermatozoa (Palmer et al 1990). In support of these observations, our current work shows that an antibody to core histones decorated similar intra-nuclear structures (Fig.…”

Section: Saida and Otherssupporting

confidence: 70%

“…However, even though sperm chromatin is highly condensed there is evidence that it is also highly organised, with the location of domains that contain particular chromosomal regions being conserved from sperm to sperm (Zalensky et al 1995, Hazzouri et al 2000. Recent work also suggests that regions accessible to exogenously applied endonucleases contain sequences that differ in gene composition from the bulk chromatin.…”

Section: Discussionmentioning

confidence: 99%

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Key gene regulatory sequences with distinctive ontological signatures associate with differentially endonuclease-accessible mouse sperm chromatin

Saida

Iles²,

Elnefati³

et al. 2011

Reproduction

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Using a well-established endonuclease-based chromatin dissection procedure in conjunction with both experimental comparative genome hybridisation (CGH) array profiling and in silico data mining, we show that mouse spermatozoa contain chromatin that is sensitive and resistant to digestion with micrococcal nuclease (MNase). Sequences represented in the micrococcal nuclease digestion solubilised (MNDS) but not the MND insoluble (MNDI) chromatin are strongly enriched in chromosomal regions of high gene density. Furthermore, by fluorescence in situ hybridisation (FISH) analysis, we show that MNDS and MNDI DNAs occupy distinct domains of decondensed mouse sperm nuclei that may also retain abundant histones. More detailed in silico analysis of CGH probe location in relation to known promoters and sequences recognised by CCCTC binding factor (CTCF) shows a significant excess of both in MNDS chromatin. A functional analysis of gene promoters reveals strong ontological signatures for ion transport on methylated promoters associated with CTCF binding sequences in MNDS chromatin. Sensory perception is the only strong ontological signature present in MNDI chromatin, driven by promoters that are not associated with CTCF regardless of their methylation status.

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Section: Saida and Otherssupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Key gene regulatory sequences with distinctive ontological signatures associate with differentially endonuclease-accessible mouse sperm chromatin

Saida

Iles²,

Elnefati³

et al. 2011

Reproduction

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“…Telomeres are localized at the nuclear periphery where theyinteract in dimers and tetramers (Zalensky et al 1995,Meyer-Ficca et al 1998,Hazzouri et al 2000.…”

Section: Introductionmentioning

confidence: 99%

Non-random positioning of chromosomes in human sperm nuclei

2004

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In human spermatozoa, the arrangement of chromosomes is non-random. Characteristic features are association of centromeres in the interior chromocenter and peripheral location of telomeres. In this paper, we have investigated the highest level of order in DNA packing in sperm ^ absolute and relative intranuclear chromosome positioning. Asymmetrical nuclear shape, existence of a defined spatial marker, and the haploid complement of chromosomes facilitated an experimental approach using in situ hybridization. Our results showed the tendencyfor non-random intranuclear location of individual chromosome territories. Moreover, centromeres demonstrated specific intranuclear position, and were located within a limited area of nuclear volume. Additionally, the relative positions of centromeres were non-random; some were found in close proximity, while other pairs showed significantly greater intercentromere distances. Therefore, a unique and specific adherence mayexist between chromosomes in sperm. The observed chromosome order is discussed in relation to sperm nuclei decondensation, and reactivation during fertilization.

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“…An increased rate of sex chromosomal anomalies has been reported in human babies conceived by ICSI [42]. This increase could be due to the fact that the sex chromosome X was found to be preferentially localized at the subacrosomal region of the sperm [8,10,43]. It may be suggested that these anomalies, at least in part, are connected with delayed chromosome processing of sperm chromosomes residing in the apical tip [43].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies clearly demonstrated that chromosomes in mature human spermatozoa have nonrandom, well-defined, and unique spatial organization [5][6][7]. In addition, sperm chromosomes have a preferred intracellular positioning [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of human male pronuclear development in a heterologous ICSI model

Jones

Mudrak

Zalensky

2010

J Assist Reprod Genet

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Purpose To evaluate human sperm nuclear chromatin decondensation in a heterologous ICSI system using hamster ova injected with human sperm. Materials and methods Frozen hamster oocytes were injected with Triton X-100 treated sperm and fixed at different time points post ICSI. Oocytes injected with nontreated sperm served as controls. Male pronuclear decondensation was evaluated after staining with DAPI. Results Sperm cells with partially destroyed membranes and depletion of the acrosome decondense more rapidly and to a greater extent than membrane/acrosome intact cells. Marked variability in pronuclear size was observed for any time point post ICSI, which most probably reflects the heterogeneity in the mature human sperm population. Conclusion Remodeling of male gamete nuclei in this heterologous ICSI mimics events that occur during natural fertilization in humans and therefore this approach may be used for studies of human sperm chromosomes transformations.

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Genome organization in the human sperm nucleus studied by FISH and confocal microscopy

Cited by 86 publications

References 54 publications

Key gene regulatory sequences with distinctive ontological signatures associate with differentially endonuclease-accessible mouse sperm chromatin

Key gene regulatory sequences with distinctive ontological signatures associate with differentially endonuclease-accessible mouse sperm chromatin

Non-random positioning of chromosomes in human sperm nuclei

Kinetics of human male pronuclear development in a heterologous ICSI model

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