Non-Protein Thiol Compounds and Antioxidant Responses Involved in Bryophyte Heavy-Metal Tolerance

Salbitani, Giovanna; Maresca, Viviana; Cianciullo, Piergiorgio; Bossa, Rosanna; Carfagna, Simona; Basile, Adriana

doi:10.3390/ijms24065302

Cited by 14 publications

(8 citation statements)

References 147 publications

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“…In this work, the higher content of phenolic compounds in leaves treated with a combination of ozone and nitrogen has been related to the formation of active oxygen radicals in vegetable systems [ 24 ]. At the same time, reduced glutathione has been identified as a tripeptide of glutamate, cysteine, and glycine with a role as a precursor of phytochelatins (PC), in redox signaling, ion homeostasis, and sulfur assimilation [ 25 ]. Generally, GSH reduces the content of H 2 O 2 and lipid peroxides via peroxidase-catalyzed reactions.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Response Mechanisms Sustain the Antibacterial and Antioxidant Activity of Quercus ilex

Verrillo,

Cianciullo,

Cozzolino

et al. 2024

Plants

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The development of new natural antibiotics is considered as the heart of several investigations in the nutraceutical field. In this work, leaves of Quercus ilex L. treated by tropospheric ozone (O3) and nitrogen (N) deposition, exhibited a clear antimicrobial efficacy against five multi-drug resistant (MDR) bacterial strains (two gram-positive and three gram-negative). Under controlled conditions, it was studied how simulated N deposition influences the response to O3 and the antibacterial and antioxidant activity, and antioxidant performance. The extraction was performed by ultra-pure acetone using two different steps. A higher antioxidant activity was measured in the presence of interaction between O3 and N treatments on Quercus leaves. At the same time, all organic extracts tested have shown bacteriostatic activity against all the tested strains with a MIC comprised between 9 and 4 micrograms/mL, and a higher antioxidant efficacy shown by spectrophotometric assay. Stronger antimicrobial activity was found in the samples treated with O3, whereas N-treated plants exhibited an intermediate antibacterial performance. This performance is related to the stimulation of the non-enzymatic antioxidant system induced by the oxidative stress, which results in an increase in the production of antimicrobial bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress Response Mechanisms Sustain the Antibacterial and Antioxidant Activity of Quercus ilex

Verrillo,

Cianciullo,

Cozzolino

et al. 2024

Plants

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“…High concentrations of intracellular Ni and Pb affect various metabolic pathways, because of their strong affinity for the –SH functional groups of essential biomolecules, negatively affecting biological metabolism and plant growth [ 14 , 33 ]. Therefore, restricting the entry of Ni and Pb is important for Ni and Pb stress tolerance in plants.…”

Section: Discussionmentioning

confidence: 99%

“…In general, plants produce low-molecular-weight thiols, such as GSH, PCs, and MT, which have a high affinity for HMs to reduce the toxicity of free HM ions in plant cells, conferring HM tolerance to plants [ 11 , 14 ]. Ni-elevated GSH synthesis enhances Ni tolerance in some plants, such as mustard, Arabidopsis , and Thlaspi goesingense [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The expression levels of genes associated with the syntheses of GSH (GSH synthetase, GS), PC (PC synthase, PCS), and MT in plants are usually dynamic in response to HM stress. These genes play pivotal roles in the detoxification and tolerance of HMs [ 11 , 12 , 13 , 14 ]. Moreover, high HM concentrations in plant cells induce oxidative stress by promoting ROS generation, which can damage cellular macromolecules, including lipids, proteins, and DNA, and cause the denaturation of cell structures and membranes, leading to cell death and plant growth inhibition [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Glycine-Rich RNA-Binding Protein AtGRP7 Functions in Nickel and Lead Tolerance in Arabidopsis

Kim,

Safdar,

Kang

et al. 2024

Plants

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Plant glycine-rich RNA-binding proteins (GRPs) play crucial roles in the response to environmental stresses. However, the functions of AtGRP7 in plants under heavy metal stress remain unclear. In the present study, in Arabidopsis, the transcript level of AtGRP7 was markedly increased by Ni but was decreased by Pb. AtGRP7-overexpressing plants improved Ni tolerance, whereas the knockout mutant (grp7) was more susceptible than the wild type to Ni. In addition, grp7 showed greatly enhanced Pb tolerance, whereas overexpression lines showed high Pb sensitivity. Ni accumulation was reduced in overexpression lines but increased in grp7, whereas Pb accumulation in grp7 was lower than that in overexpression lines. Ni induced glutathione synthase genes GS1 and GS2 in overexpression lines, whereas Pb increased metallothionein genes MT4a and MT4b and phytochelatin synthase genes PCS1 and PCS2 in grp7. Furthermore, Ni increased CuSOD1 and GR1 in grp7, whereas Pb significantly induced FeSOD1 and FeSOD2 in overexpression lines. The mRNA stability of GS2 and PCS1 was directly regulated by AtGRP7 under Ni and Pb, respectively. Collectively, these results indicate that AtGRP7 plays a crucial role in Ni and Pb tolerance by reducing Ni and Pb accumulation and the direct or indirect post-transcriptional regulation of genes related to heavy metal chelators and antioxidant enzymes.

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“…Nonprotein thiol compounds, such as glutathione and phytochelatins (PCs), are directly involved in the fight against toxic metal/metalloid stress. Under exposure to toxic metals/metalloids, these compounds can chelate toxic metals/metalloids and then transport them into vacuoles via ABC-like transporters, transport proteins found in all kingdoms of life, including plants, thereby leading to a reduction in the number of metals present in the cytoplasm, which, in turn, shields the plants from the harmful consequences associated with these metals [42].…”

Section: Role Of Transporters and Chelatorsmentioning

confidence: 99%

Heavy Metal Contamination in Soil: Implications for Crop Resilience and Abiotic Stress Management

Almotairy

2024

Abiotic Stress in Crop Plants - Ecophysiological Responses and Molecular Approaches

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This chapter rigorously examines soil toxic metal/metalloid contamination and its profound implications on crop resilience, focusing on abiotic stress conditions. It begins by elucidating the natural and anthropogenic origins of soil contamination, illustrating how plants absorb these toxicants, and elaborating on their physio-molecular responses. The chapter accentuates the detrimental manifestations of impaired photosynthesis, nutrient uptake, and oxidative stress management, underscoring the urgent need for effective mitigation strategies. Phytoremediation and genetic engineering advancements are explored as promising strategies to optimize plant resilience in contaminated environments. Novel methodologies, including phytochelatins and the strategic application of genetic engineering, demonstrate potential in improving plant growth and resilience, showcasing significant advancements toward sustainable agricultural practices. Moreover, the interaction between plants and soil microbes is dissected, revealing a symbiotic relationship that influences the bioavailability of toxic metals/metalloids and optimizes plant health under stress conditions. This insight into microbial assistance opens new avenues for research and application in crop management and soil remediation. This chapter contributes essential knowledge toward bolstering crop resilience against toxic metal/metalloid contamination by presenting cutting-edge research findings and sophisticated mitigation techniques. It emphasizes the critical role of innovative research in overcoming the challenges posed by soil contamination, paving the way for achieving sustainable agricultural productivity and food security in the face of environmental stressors.

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Non-Protein Thiol Compounds and Antioxidant Responses Involved in Bryophyte Heavy-Metal Tolerance

Cited by 14 publications

References 147 publications

Oxidative Stress Response Mechanisms Sustain the Antibacterial and Antioxidant Activity of Quercus ilex

Oxidative Stress Response Mechanisms Sustain the Antibacterial and Antioxidant Activity of Quercus ilex

Glycine-Rich RNA-Binding Protein AtGRP7 Functions in Nickel and Lead Tolerance in Arabidopsis

Heavy Metal Contamination in Soil: Implications for Crop Resilience and Abiotic Stress Management

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