Non-lethal heat shock induces Hsp70 synthesis and promotes tolerance against heat, ammonia and metals in post-larvae of the white leg shrimp Penaeus vannamei (Boone, 1931)

Sung, Yeong Yik; Rahman, Nooridayu Abdul; Shazili, Noor Azhar Mohamed; Chen, Shuaijun; Lv, Aijun; Sun, Jinfeng; Shi, Hongyue; MacRae, Thomas H.

doi:10.1016/j.aquaculture.2017.09.034

Cited by 33 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HSPs are well-known for their quick response to environmental stresses (Lewis et al, 2016; Sung et al, 2018). However, the respective roles of HSP40 in response to a particular environmental stress have not been illustrated in L. vannamei to date, even though the expression profiles of other HSPs in L. vannamei , such as HSP60 , HSP70 and HSP90 , were reported under acute thermal stress, pH challenge and heavy metal exposure (Qian et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In Metapenaeus ensis , HSC70 (the constitutive form of HSP70) was found to be a negative regulator of vitellogenesis (Chan et al, 2014). In L. vannamei , HSP10 , HSP27 , HSP30 , HSP60 , HSP70 and HSP90 mRNA were consistently or specifically expressed in response to thermal or pH stress, heavy metal exposure (Qian et al, 2012), and bacterial or viral infection (Junprung et al, 2017; Yuan et al, 2017); HSP70 promoted post-larval tolerance to heat, ammonia and metals (Sung et al, 2018), and HSC70 reduced protein aggregation and mortality after viral infection (Yuan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Chen

Lin

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

Heat shock proteins (HSPs), a family of conserved proteins that are produced by cells in response to stresses, are known as molecular chaperones with a range of housekeeping and cellular protective functions. The 40 kD heat shock protein (HSP40) is a co-chaperone for HSP70 in the regulation of ATP hydrolysis. Unlike its well-documented cofactor HSP70, little is currently known regarding the biological functions of HSP40 in crustacean species such as penaeid shrimp. In the present study, the cDNA encoding HSP40 (Lv-HSP40) was identified from the Pacific white shrimp Litopenaeus vannamei, a highly significant commercial culture species. The structural organization indicates that Lv-HSP40 belongs to the type-I HSP40s. The muscle, gill, and hepatopancreas are the main sites of Lv-HSP40 transcript expression. Within these tissues, Lv-HSP40 mRNA were predominantly exhibited in the myocytes, epithelial cells and hepatopancreatic cells, respectively. Under acute thermal stress in the culture environment, Lv-HSP40 transcript levels are significantly induced in these three tissues, while low pH stress only upregulates Lv-HSP40 mRNA in the hepatopancreas and gill. During ontogenesis, Lv-HSP40 transcript levels are high at early embryonic stages and drop sharply at late embryonic and early larval stages. The ovary is another major organ of Lv-HSP40 mRNA expression in female shrimp, and Lv-HSP40 transcripts were mainly presented in the follicle cells but only weekly detected in the oocytes. Ovarian Lv-HSP40 mRNA levels increase continuously during gonadal development. Silencing of the Lv-HSP40 gene by RNA interference may effectively delay ovarian maturation after unilateral eyestalk ablation. The roles of Lv-HSP40 in ovarian development are speculated to be independent of its cofactor HSP70, and the vitellogenesis factor vitellogenin (Vg) and vitellogenin receptor (VgR). Our study, as a whole, provides new insights into the roles of HSP40 in multiple physiological processes in L. vannamei: (1) HSP40 is a responding factor during stressful conditions; and (2) HSP40 participates in embryonic and ovarian development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Chen

Lin

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that environmental stress (such as heat stress, heavy metals, and ammonia nitrogen) can promote the synthesis of the heat shock protein HSP70 ( 48 , 49 ). HSP70 can effectively reduce damage to the body caused by stress by preventing protein folding and degrading denatured protein.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

et al. 2021

View full text Add to dashboard Cite

The “milky disease” of the Chinese mitten crab, Eriocheir sinensis, is a highly lethal fungal disease caused by Metschnikowia bicuspidata infection. To elucidate the immune responses of the hemolymph of E. sinensis to M. bicuspidata infection, a comparative analysis of the hemolymph of E. sinensis infected with M. bicuspidata and that treated with phosphate buffered saline was performed using label-free quantitative proteomics. A total of 429 proteins were identified. Using a 1.5-fold change in expression as a physiologically significant benchmark, 62 differentially expressed proteins were identified, of which 38 were significantly upregulated and 24 were significantly downregulated. The upregulated proteins mainly included cytoskeleton-related proteins (myosin regulatory light chain 2, myosin light chain alkali, tubulin α-2 chain, and tubulin β-1 chain), serine protease and serine protease inhibitor (clip domain-containing serine protease, leukocyte elastase inhibitor, serine protein inhibitor 42Dd), catalase, transferrin, and heat shock protein 70. Upregulation of these proteins indicated that phenoloxidase system, phagocytosis and the ROS systems were induced by M. bicuspidata. The downregulated proteins were mainly organ and tissue regeneration proteins (PDGF/VEGF-related factor protein, integrin-linked protein kinase homing pat-4 gene) and hemagglutination-associated proteins (hemolymph clottable protein, hemocyte protein-glutamine gamma-glutamyltransferase). Downregulation of these proteins indicated that M. bicuspidata inhibited hemocyte regeneration and hemolymph agglutination. Fifteen differentially expressed proteins related to immunity were verified using a parallel reaction monitoring method. The expression trend of these proteins was similar to that of the proteome. To the best of our knowledge, this is the first report on the proteome of E. sinensis in response to M. bicuspidata infection. These results not only provide new and important information on the immune response of crustaceans to yeast infection but also provide a basis for further understanding the molecular mechanism of complex host pathogen interactions between crustaceans and fungi.

show abstract

“…The HSPs also directly stimulate cells of the innate immune system, suggesting they are activators of the innate immune system in animals (Cui et al., 2011; Wallin et al., 2002). For example, a nonlethal heat shock induces Hsp70 synthesis and promotes the tolerance of shrimp ( Penaeus vannamei ) to stresses resulting from heat, ammonia, and metals (Sung et al., 2018) and also prevents heart failure or ageing in humans (Ranek et al., 2018). So far, several plant species’ Hsp70s have been surveyed, such as barley ( Hordeum vulgare L.) (Landi et al., 2019), Brachypodium distachyon (L.) Beauv.…”

Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of heat‐shock proteins in wheat infected with powdery mildew and stripe rust

et al. 2021

View full text Add to dashboard Cite

Heat-shock proteins (HSPs), which are encoded by conserved gene families in plants, are crucial for development and responses to diverse stresses. However, the wheat (Triticum aestivum L.) HSPs have not been systematically classified, especially those involved in protecting plants from disease. Here, we classified 119 DnaJ (Hsp40) proteins (TaDnaJs; encoded by 313 genes) and 41 Hsp70 proteins (TaHsp70s; encoded by 95 genes) into six and four groups, respectively, via a phylogenetic analysis. An examination of protein sequence alignment revealed diversity in the TaDnaJ structural organization, but a highly conserved J-domain, which was usually characterized by an HPD motif followed by DRD or DED motifs. The expression profiles of HSP-encoding homologous genes varied in response to Blumeria graminis f. sp. tritici (Bgt) and Puccinia striiformis f. sp. tritici (Pst) stress. A quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated a lack of similarity in the expression of DnaJ70b, Hsp70-30b, and Hsp90-4b in Bgt-infected resistant and susceptible wheat. Furthermore, a direct interaction between DnaJ70 and TaHsp70-30 was not detected in a yeast two-hybrid (Y2H) assay, but screening cDNA library and Y2H evidence supported that TaHsp70-30 not only interacts directly with heat-shock transcription factor (HSF) A9-like protein but also interacts with TaHsp90-4 by HSP organizing protein. This study revealed the structure and expression profiles of the HSP-encoding genes in wheat, which may be useful for future functional elucidation of wheat HSPs responses to fungal infections.

show abstract

Non-lethal heat shock induces Hsp70 synthesis and promotes tolerance against heat, ammonia and metals in post-larvae of the white leg shrimp Penaeus vannamei (Boone, 1931)

Cited by 33 publications

References 44 publications

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

Identification and expression analysis of heat‐shock proteins in wheat infected with powdery mildew and stripe rust

Contact Info

Product

Resources

About