Non-labeled monitoring of targeted liposome interactions with a model receptor surface: Effect of flow rate and water content

Liang, Huamin; Tuppurainen, Jussipekka; Lehtinen, Julia

doi:10.1016/j.ejps.2013.08.011

Cited by 9 publications

(5 citation statements)

References 53 publications

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“…Liang et al 16 investigated the effect of flow rate and water content on targeted liposome interactions via surface plasmon resonance (SPR) spectroscopy and quartz crystal microbalance (QCM) studies. They found that increasing the flow rate decreases the maximum amount of bound liposomes and the equilibrium constant, as does decreasing the water content in the bound vesicle layer.…”

Section: Introductionmentioning

confidence: 99%

“…The binding studies were performed using SPR spectroscopy that has been shown to be the ideal tool for label-free monitoring of such interactions in real-time resolution. 16,17,31 Further, the influence of various temperatures on the binding behaviour was analysed. With our present work, we will broaden the knowledge on liposome-solid surface interactions; this will benefit a wide range of applications by providing clever surface engineering strategies preventing non-specific adsorption without the need of bulk blocking or surface coating.…”

Section: Introductionmentioning

confidence: 99%

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Investigating non-specific binding to chemically engineered sensor surfaces using liposomes as models

Fenzl

Genslein

Domonkos

et al. 2016

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Nanoparticles are ubiquitously used for signal enhancement in (bio)sensors, but their true possible performance is typically hampered by non-specific binding. A better understanding of the nature and the prevention of non-specific binding through surface engineering of the particles and sensor surfaces is needed to intelligently design (bio)sensors and potentially avoid bulk blocking methods. Hence, two types of liposomes were used as model for signal-enhancing nanoparticles. Their surface was engineered to bear negative surface charge. One type was synthesized with additional 6 mol% -COOH groups. Their interaction with four typical chemically modified sensor surfaces was then mechanistically characterized by surface plasmon resonance (SPR) spectroscopy. It was shown that the non-specific binding can be described with Langmuir isotherms providing quantitative information of dissociation constants and surface loading with especially high correlation coefficients (>0.97) for all the studied sensor surfaces modified with hydrophilic alkane thiols. By tailoring the sensor surface chemistry, non-specific binding was significantly minimized. Here, carboxyl- or methyl-terminated surfaces performed best. In fact, the pairing of -COOH groups on the sensor surface with -COOH groups on the liposomes almost completely eliminated non-specific binding, resulting in a SPR signal change of only 1 mRIU (refractive index unit) at 100 μM phospholipid concentration. Surprisingly though, -OH groups on the surface, which are also commonly used in sensing applications, did not lead to decreased adsorption, but caused significant signal changes (4 mRIU at 100 μM phospholipid) due to non-specific binding. Overall, the mechanistic studies presented here demonstrate that by careful design of the nanoparticle surface and by choosing sensor surfaces with terminal -CH3 or -COOH groups, improved sensing (micro)systems with very low non-specific adsorption can be obtained.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating non-specific binding to chemically engineered sensor surfaces using liposomes as models

Fenzl

Genslein

Domonkos

et al. 2016

Analyst

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“…The protocol for SAM establishment on a plain gold sensor surface was adopted from previous works describing the robustness and versatility of such molecular construction. [58] In brief, the gold sensor was incubated overnight ex situ in a glass vial sealed with wax membrane in MuOH: Biotin-PEG-Thiol mixture in molar ratio 90%:10% in 88% EtOH. Immediately before using, the sensor was washed three times with excess amount of 99% EtOH and gently dried under nitrogen gas flow and mounted into the instrument.…”

Section: Methodsmentioning

confidence: 99%

Targeting Tumor‐Associated Exosomes with Integrin‐Binding Peptides

et al. 2017

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All cells expel a variety of nano-sized extracellular vesicles (EVs), including exosomes, with composition reflecting the cells’ biological state. Cancer pathology is dramatically mediated by EV trafficking via key proteins, lipids, metabolites, and microRNAs. Recent proteomics evidence suggests that tumor-associated exosomes exhibit distinct expression of certain membrane proteins, rendering those proteins as attractive targets for diagnostic or therapeutic application. Yet, it is not currently feasible to distinguish circulating EVs in complex biofluids according to their tissue of origin or state of disease. Here we demonstrate peptide binding to tumor-associated EVs via overexpressed membrane protein. We find that SKOV-3 ovarian tumor cells and their released EVs express α3β1 integrin, which can be targeted by our in-house cyclic nonapeptide, LXY30. After measuring bulk SKOV-3 EV association with LXY30 by flow cytometry, Raman spectral analysis of laser-trapped single exosomes with LXY30-dialkyne conjugate enabled us to differentiate cancer-associated exosomes from non-cancer exosomes. Furthermore, we introduce the foundation for a highly specific detection platform for tumor-EVs in solution with biosensor surface-immobilized LXY30. LXY30 not only exhibits high specificity and affinity to α3β1 integrin-expressing EVs, but also reduces EV uptake into SKOV-3 parent cells, demonstrating the possibility for therapeutic application.

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“…Bionavis SPR Navi 200 MP-SPR device and Bionavis thin glass substrates (Schott D263T glass slides) coated with gold layers were used to detect the resonance angle SPR (RA-SPR) signal resulting from the biomolecule and the gold particle binding. The experimental procedures were based on literatureestablished methods 34 . The sensors were fabricated by first cleaning the glass substrate using reactive ion etching (RIE) with O2 plasma (20 W, 15 s), and then the sample was placed inside JYU self-made UHV e-beam evaporator.…”

Section: Protein and Nanoparticle Attachment To Gold Surface In Spr Smentioning

confidence: 99%

“…1i-k). The SPR measurements were carried out using standard procedures (see Methods section) 34 . In each deposition step, a 16 min injection of the proteins or polymers to the sensor surface was followed by flushing of the measurement chamber using phosphate buffered saline (PBS) buffer, followed by next injection.…”

Section: Assembly Of the Dna-aunp Nanoactuatormentioning

confidence: 99%

A DNA–nanoparticle actuator enabling optical monitoring of nanoscale movements induced by an electric field

et al. 2018

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Merging biological and non-biological matter to fabricate nanoscale assemblies with controllable motion and function is of great interest due to its potential application, for example, in diagnostics and biosensing. Here, we have constructed a DNA-based bionanoactuator that interfaces with biological and non-biological matter via an electric field in a reversibly controllable fashion. The read-out of the actuator is based on motion-induced changes in the plasmon resonance of a gold nanoparticle immobilized to a gold surface by single stranded DNA. The motion of the gold nanoparticle and thus the conformational changes of the DNA under varying electric field were analyzed by dark field spectroscopy. After this basic characterization, another actuator was built utilizing hairpin-DNA coated gold nanoparticles, where the hairpin-DNA induced discrete transitions between two specific open-loop and folded-loop states. These two states and the transition dynamics between them were clearly visible in the actuator behavior. The demonstrated nanoactuator concept could be readily extended to inspection of conformational changes of other biomolecules as well. Besides, this concept enables other possibilities in applications like surface-enhanced Raman spectroscopy and fluorescence enhancement, since the specific wavelength of the plasmon resonance of the actuator can be tuned by the external voltage.

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Non-labeled monitoring of targeted liposome interactions with a model receptor surface: Effect of flow rate and water content

Cited by 9 publications

References 53 publications

Investigating non-specific binding to chemically engineered sensor surfaces using liposomes as models

Investigating non-specific binding to chemically engineered sensor surfaces using liposomes as models

Targeting Tumor‐Associated Exosomes with Integrin‐Binding Peptides

A DNA–nanoparticle actuator enabling optical monitoring of nanoscale movements induced by an electric field

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