Non-jejuni/coli Campylobacter Species and Related Organisms in Poultry, and Their Relevance in Human and Animal Disease

Lastovica, Albert J.

doi:10.1007/978-3-319-29907-5_11

Cited by 6 publications

(9 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reported evidences show that the smoker patients displayed a decrease of oxygen tension in the oral cavity, creating favorable environmental conditions in the periodontal pocket for the colonization of certain periodontopathogens (ABUSLEME et al, 2013;NICOLIĆ-JAKOBA et al, 2012). Therefore, it can be suggested that the oxygen conditions are important for C. gracilis, C. rectus and C. concisus and, in this oxygen reduced subgingival environment, they could increase the prevalence of C. gracilis for being a strict microaerobic microorganism (LASTOVICA, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The etiology of periodontal diseases is multifactorial, where various factors such as host, environment and infectious agents are involved (PEREZ-CHAPARRO et al, 2014;HENNE et al, 2014;VARGAS SEGURA et al, 2015;SRINIVASAN, 2016;TOMAS et al, 2016;MEURIC et al, 2017). Campylobacter species have been isolated from humans and animals, being also associated with this disease (LASTOVICA, 2016). In humans, several species have been implicated as etiologic agents of periodontal disease (PEREZ-CHAPARRO et al, 2014, HENNE et al, 2014VARGAS SEGURA et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Campylobacter species in chilean patients with chronic periodontitis

Montanares¹,

Opazo²,

Herrera³

et al. 2018

Biosci. J.

View full text Add to dashboard Cite

The purpose of this study is to identify the presence of Campylobacter species (C. rectus, C. concisus, C. gracilis and C. ureolyticus) in Chilean patients with chronic periodontitis, and to establish the relationship of these microorganisms with the periodontal conditions of smoker and non-smoker patients. Subgingival plaque samples were collected from four periodontal-affected tissue sites of 15 smoker and 15 non-smoker patients with chronic periodontitis. A sample per quadrant was obtained, with a probing depth of ≥6 mm and an insertion loss of >3 mm in each patient. Polymerase chain reaction (PCR) was performed with the specific 16S rDNA primers for the molecular detection of C. rectus and C. gracilis, specific cpn60 primers for C. concisus and hsp60 primers gene for C. ureolyticus. Campylobacter species showed an overall prevalence of 93.3% in periodontal patients, while C. rectus was the most frequent one (80%), followed by C. concisus (66.7%), C. gracilis (33.3%), and C. ureolyticus (10%). Only C. gracilis showed a statistically significant (p = 0.002) association with chronic periodontitis among samples from smoker and nonsmoker patients. A high prevalence of the Campylobacter genus in the analyzed populations (93.3%) was found, being C. rectus the most frequent (80%) species. Besides, C. gracilis showed a statistically significant association between smoker state and chronic periodontitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Campylobacter species in chilean patients with chronic periodontitis

Montanares¹,

Opazo²,

Herrera³

et al. 2018

Biosci. J.

View full text Add to dashboard Cite

show abstract

“…After this time, Arcobacter isolation was performed using the membrane filtration technique 21 . Briefly, 100 mL of enrichment media were inoculated onto a sterile 0.45 µm pore size nitrocellulose membrane filter placed on the surface of non-selective blood agar plates, based on the Cape Town protocol 22 . Plates were left at room temperature for one hour.…”

mentioning

confidence: 99%

First isolation report of Arcobacter cryaerophilus from a human diarrhea sample in Costa Rica

Barboza¹,

Cubillo²,

Castro

et al. 2017

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Arcobacter cryaerophilus is an emerging enteropathogen and potential zoonotic agent transmitted by food and water. In Costa Rica, this bacterium has not been associated with cases of human gastroenteritis, even though it has been isolated from farm animals, especially poultry. This paper reports the first isolation of A. cryaerophilus from a human case of bloody watery diarrhea and the virulence genes associated with this isolate.

show abstract

“…Lastovica et al [16] reported that C. concisus was the second species with a higher prevalence after C. jejuni. However, C. concisus has been isolated from diarrheic patients without other pathogenic microorganisms suggesting it to be an emerging cause of human gastroenteritis [16,57].…”

Section: Discussionmentioning

confidence: 99%

“…However, atypical Campylobacter species are gaining considerable attention as important human and animal pathogens [13][14][15]. Infections caused by Campylobacter are usually under-reported due to difficulties in isolation procedures [16,17]. For instance, it has been estimated that 40% of the bacteria from human faeces diagnosed through microscopy cannot be cultured in the laboratory [17].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania

Gahamanyi

Mboera

Matee

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: A growing number of Campylobacter species other than C. jejuni and C. coli have been considered as emerging human and animal pathogens. However, the contribution of these species to human gastroenteritis is poorly documented. This study aimed at detecting Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania using Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene, and Sanger sequencing . Methods: A total number of 100 faecal samples (70 from human and 30 from cattle) were collected from diarrheic and non-diarrheic patients and healthy cattle in Kilosa district, Tanzania from July to October 2019. Species identification was conducted by PCR and 16S rRNA sequencing. The phylogenetic analysis was carried out by comparison of the 16S rRNA gene sequences to reference strains by the Neighbor-Joining method in MEGA X. Results: Campylobacter species detection rate by PCR was 65.7% (46/70) and 20% (6/30) in humans and cattle, respectively. There were five human diarrheic cases, four showed Campylobacter presence and two were from children ≤15 years of age. In humans, the 16S rRNA sequencing revealed that C. concisus was the most predominant species occurring at a frequency of 37.8% (14/37), followed by uncultured Campylobacter spp. 24.3% (9/37) and C. hominis 21.6% (8/37). The least represented species were C. jejuni and C. lanienae all occurring at 2.7% (1/37). In cattle, five (100%) sequenced PCR products matched with C. lanienae . Phylogenetic analysis revealed that Campylobacter 16S rRNA sequences were closely related to C. concisus , uncultured Campylobacter sp., C. hominis , and C. gracilis . Conclusion: The non- C. jejuni / C. coli species are present in human and cattle faecal samples and their true occurrence is probably under-reported due to shortcomings of conventional techniques used in most diagnostic microbiology laboratories. Based on our findings, we recommend that molecular techniques be adopted for direct detection of Campylobacter species during routine laboratory screening and surveillance studies. Keywords: Campylobacter , molecular diagnostics, polymerase chain reaction, sequencing, gastroenteritis, Tanzania

show abstract

Non-jejuni/coli Campylobacter Species and Related Organisms in Poultry, and Their Relevance in Human and Animal Disease

Cited by 6 publications

References 100 publications

Campylobacter species in chilean patients with chronic periodontitis

Campylobacter species in chilean patients with chronic periodontitis

First isolation report of Arcobacter cryaerophilus from a human diarrhea sample in Costa Rica

Molecular detection of Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania

Contact Info

Product

Resources

About