Non-invasive Method for Evaluation of Gastrocecal Transit Time by Using a Breath Test in Conscious Rats

Abstract

The aim of this study was to establish a method for evaluating gastrocecal transit time using a simple and noninvasive 13C breath-test system in conscious rats. After fasting, rats were orally administered lactose-[13C]ureide. 13CO2 level in the expired air was measured at appropriate intervals. It was confirmed that priming dose of lactose-ureide was necessary for determining gastrocecal transit time. The level of 13CO2 in the expired air increased in a lactose-[13C]ureide dose-dependent manner. The gastrocecal transit time determined with 30 mg/kg of lactose-[13C]ureide was 160+/-24.5 min. This method is expected to contribute to the further understanding of intestinal pathophysiology.

Simultaneous measurement of gastric emptying and gastrocecal transit times in conscious rats using a breath test after ingestion of [1-¹³C] acetic acid and lactose-[¹³C] ureide

“…We also confirmed that a priming dose of lactose-ureide is necessary in the breath testing of rats (Uchida et al, 2009a). Therefore, the first phase of each experiment involved evaluating the effect of this priming dose of lactose-ureide on gastric emptying.…”

“…Ruemmele et al (1997) reported that human gut tissues possess no allantoicase-like activity. In basic research, we reported that a priming dose of lactose-ureide is also needed for rats to enable the investigation of gastrocecal transit time using lactose-[ 13 C] ureide (Uchida et al, 2009a). Therefore, we evaluated at first the effects of a priming dose of lactose-ureide and the simultaneous administration of lactose-ureide instead of lactose-[ 13 C] ureide on gastric emptying.…”

“…The aspiration volume was again set at 150 mL/min. The gastrocecal transit time was essentially defined as in our earlier experiments (Uchida et al, 2009a) but with a slight modification. We defined gastrocecal transit time as the time when the measured value of 13 CO 2 was greater than the mean + 2 S.D.…”

“…Moreover, we have also established a non-invasive and feasible method for the evaluation of rat gastrocecal transit time by using lactose-[ 13 C] ureide (Uchida et al, 2009a) and the effect of atropine sulfate which significantly delayed the gatsrocecal transit time (Uchida et al, 2009b).…”

Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1- 13 C]acetic acid and lactose-[ 13 C]ureide breath test in conscious rats

“…On the other hand, in basic research we developed a simple and non-invasive breath test system for evaluating gastric emptying and pancreatic exocrine secretion in conscious rats [6][7][8]. Recently, we also reported a non-invasive and feasible method for the evaluation of the rat gastrocecal transit time by using lactose-[ 13 C]ureide [9]. However, the drug effect affecting the gastrocecal transit time has not been evaluated.…”