Non-invasive in vivo infrared laser spectroscopy to analyse endogenous oxy-haemoglobin, deoxy-haemoglobin, and blood volume in the rat CNS

Crespi, Francesco; Bandera, Andrea; Donini, Maurizio; Heidbreder, Christian; Rovati, Luigi

doi:10.1016/j.jneumeth.2004.11.016

Cited by 17 publications

(12 citation statements)

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“…These results strength the applicability of NIRS for measurement of turnover of endogenous oxygen that is directly related to neuronal functions coupled with blood flow. In particular they confirm NIRS capabilities on the direct non invasive detection of HbO 2 and Hb in rat brain as proposed earlier [11]. The ligand-receptor interaction triggers neuronal activity which is linked to an increase in metabolism (metabolic coupling).…”

Section: Discussionsupporting

confidence: 86%

“…Each rat was anaesthetised using urethane (1.4 g/kg ip) and placed on a stereotaxic apparatus [D. Kopf, USA]. Then the input system (four optic fibres, 200 μm diameter each) and the receiver system were both firmly placed using a stereotaxic micromanipulator onto the surface of the rat's head, close to the sagittal line without any surgery as already shown [11].…”

Section: Methodsmentioning

confidence: 99%

“…Calibration of the NIRS prototype was performed as reported earlier [10,11] then following a 10min control/control recording period, and in order to evaluate the sensitivity of NIRS measurements to exogenous oxygen, rats were supplied with pure O 2 [n=6, 0.5 bar, 2 min] via direct insufflation into the animal mouth. Successively, the anaesthetized adult male rats prepared for NIRS analysis in the "whole The model used to support the measurement executed with the NIRS instrument is based on the modified equation of Lambert-Beer:…”

Section: Methodsmentioning

confidence: 99%

“…so that analysis of brain vascular and metabolic activities in real time can be performed in the CNS of anaesthetized rats [11]. Indeed, HbO 2 and Hb are chromophores also present in brain tissue and are markers of the degree of brain tissue oxygenation, thus providing an index of brain tissue metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Parallel Effect of Nicotine and MK-801 on Brain Metabolism: An In vivo Non Invasive Near-Infrared Spectroscopy Analysis in Rats

Crespi¹

2013

Curr Synthetic Sys Biol

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Oxygenated haemoglobin, deoxyhaemoglobin and total blood volume are measured with our recently developed Near-Infrared Spectroscopy [NIRS] apparatus allowing in vivo non invasive real time monitoring of brain metabolism in anaesthetized rats. These measurements are indicative of the state of vascular activity and the state of the oxygen saturation, thus of the level of metabolism in the living tissue.Nicotine is a natural alkaloid derived from tobacco that has been implicated in various effects ranging from addiction to toxic effects and neuro-protective actions. Here we analyse the influence of nicotine as well as that of the non competitive NMDA receptor antagonist MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate)] upon brain metabolism in anaesthetized rats.In the first 30min after treatment, nicotine decreases significantly although in a transient manner oxygenated haemoglobin and total blood volume while increasing significantly deoxyhaemoglobin. In addition, MK-801 performed in another group of rats was followed by changes in these three parameters that were similar to those monitored in nicotine treated rats.The NIRS methodology appears to be apt to analyzing non-invasively and in real time the influence of systemic pharmacological treatments upon brain metabolism. In particular the data gathered show similarity of action of the two chemicals studied on influencing metabolic brain "behaviour" proposing that their central protective action may pass via the observed similar changes in brain metabolism. These changes could be a common mechanism of adaptation and protection towards neurotoxicity: mechanism that should be also considered in the intent of developing new pharmaceutical approaches for neuro-protective treatments.

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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