Non-enzymatic screen-printed sensor based on PtNPs@polyazure A for the real-time tracking of the H2O2 secreted from living plant cells

Jiménez‐Pérez, Rebeca; Almagro, Lorena; González-Sánchez, María-Isabel; Pedreño, María A.; Valero, Edelmira

doi:10.1016/j.bioelechem.2020.107526

Cited by 18 publications

(4 citation statements)

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“…After the electrodeposition of PAA, the surface displayed a smooth morphology, which confirmed that the aSPCE was coated by the polymeric film ( Figure 2 C). The electrodeposition of PtNPs on PAA ( Figure 2 D) showed PtNPs with a non-uniform elongated shape (fusiform) with average sizes of 106 ± 17 nm (length) and 33 ± 6 nm (width), which are highly dispersed throughout the PAA film compared with the PtNPs electrodeposited on the naked aSPCE, which provided an ill-defined shape with less distributed and more aggregated PtNPs [ 30 ]. It is noteworthy that the characteristic morphology of PtNPs was lost when casting GOx on PtNPs-PAA-aSPCE ( Figure 2 E), in which distributed small enzyme clusters were observed.…”

Section: Resultsmentioning

confidence: 99%

“…The synergy between PAA and PtNPs and the high electrochemical surface obtained allowed for the development of a highly sensitive H 2 O 2 sensor at a low overpotential, with a very appropriate limit of detection, stability, and reproducibility. A variant of this electrode has also been used in the real-time monitoring of the H 2 O 2 secreted from living plant cells under different stress conditions, demonstrating the excellent applicability of the modified sensor [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Jiménez-Fiérrez

González-Sánchez

Jiménez‐Pérez

et al. 2020

Sensors

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Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H2O2. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM−1 cm−2), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Jiménez-Fiérrez

González-Sánchez

Jiménez‐Pérez

et al. 2020

Sensors

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“…[ 211 ] The emission of luminol can be enhanced by reactive radicals such as •OH and O 2 • – , [ 113,211 ] which are usually generated by H 2 O 2 decomposition under the catalytic effect of enzymes or catalysts such as HRP [ 215–217 ] and PtNPs. [ 218,219 ] For example, an ECL sensor based on Au star/bovine serum albumin (BSA)/luminol nanocomposites and PANI hydrogel decorated with PtNPs was developed for detection of human chorionic gonadotropin (HCG) (Figure 6E). [ 211 ] The specific HCG antibodies (Ab1) were loaded on PANI/PtNPs, and HCG detection antibodies (Ab2) and luminol were loaded onto the Au star/BSA nanocomposites to form a luminol/Au star/BSA/Ab2 complex.…”

Section: Working Principles Of Biosensors Based On Hydrogel Compositesmentioning

confidence: 99%

Hydrogel‐based composites: Unlimited platforms for biosensors and diagnostics

Wang

Liu

Wang

et al. 2021

VIEW

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The need for biosensing systems, which are capable of reliable and accurate detection of physiological signals and disease biomarkers along with biocompatible surface chemistry and textures for device–human interface, has driven the continuous search for advanced sensing materials, sensing strategies, and device structures. Hydrogels are hydrophilic polymers with high water content and thus akin to human tissues. They are not only able to act as polymeric matrices to load functional materials for bio‐signal transducing, but also stimuli‐responsive to cooperate with the filler materials for further enhanced sensing performance. A vast combination of hydrogels and functional fillers such as biomacromolecules, metal nanostructures, carbon nanomaterials, and two‐dimensional materials beyond graphene has been demonstrated over the last decade for fabrication of biosensors for disease diagnosis and health monitoring. This review article aims to provide an overview of the various hydrogel‐based composites, introduce their preparation protocols, and describe the working principles of their corresponding sensors. Recent development of these sensors for potential diagnosis of diseases such as diabetes, cancers, and cardiovascular diseases is then summarized, followed by stating the current challenges and prospects in this field.

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“…Nanocomposites, including Pt–TiO 2 NPs [ 16 ] and Pt–Pd nanoflakes [ 17 ] have been developed to increase the sensitivity of SPEs for significant analyte determination. A modification involving the electrodeposition of Pt NPs on a conductive layer of polyazure A-dodecyl sulphate was used for sensitive determination of hydrogen peroxide [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Facile preparation of a Pt-ERGO composite modified screen-printed electrode for the sensitive determination of phenolic compounds

Lema,

Oliveira,

Amorim

et al. 2023

Heliyon

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Non-enzymatic screen-printed sensor based on PtNPs@polyazure A for the real-time tracking of the H2O2 secreted from living plant cells

Cited by 18 publications

References 50 publications

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Hydrogel‐based composites: Unlimited platforms for biosensors and diagnostics

Facile preparation of a Pt-ERGO composite modified screen-printed electrode for the sensitive determination of phenolic compounds

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