Non-destructive quantitative 3D analysis for the optimisation of tissue scaffolds

Jones, Julian R.; Poologasundarampillai, Gowsihan; Atwood, Robert C.; Bernard, Dominique; Lee, Peter

doi:10.1016/j.biomaterials.2006.11.014

Cited by 183 publications

(129 citation statements)

References 35 publications

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“…Ongoing development of micro-CT techniques is improving qualitative and quantitative analysis of tissue engineering scaffolds. Jones et al (Jones et al, 2007) applied three algorithms to identify pores, interconnects and pore size distribution in bioceramic scaffolds to predict the permeability of the pore network and thus optimize bioreactor conditions for cell seeding. .…”

Section: Challenges In Studying Cell Behaviour On Biomaterials and Comentioning

confidence: 99%

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Chang¹,

Wang²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

312

305

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Section: Challenges In Studying Cell Behaviour On Biomaterials and Comentioning

confidence: 99%

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Chang¹,

Wang²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

312

305

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“…For example, simultaneous micro-computed tomography (micro-CT) and micromechanical testing have been used to study the behavior of tissue scaffolds under compression [3], the structural change of a strained nonwoven papermaker felt [28], and the mechanical properties of sandstone [29]; while the permeabilities of sandstones and packed bed columns have been studied by imaging the water in the void space using magnetic resonance imaging (MRI) [1,6]. While micro-CT images the porous medium itself, MRI images the void space within (e.g.…”

Section: Fig1mentioning

confidence: 99%

“…The characterization of porous media is of critical importance in a wide variety of disciplines and applications, including mathematics, soil physics, petroleum engineering, biomedical devices, and materials design [1][2][3]. It is often desired to understand the relation between the structural and the functional properties, e.g., fluid filtration properties, of the porous media.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional imaging of electrospun fiber mats using confocal laser scanning microscopy and digital image analysis

Choong

2015

J Mater Sci

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Confocal laser scanning microscopy with fluorescent markers and index matching has been used to collect three-dimensional (3D) digitized images of electrospun fiber mats and of a borosilicate glass fiber material. By embedding the fluorescent dye in either the material component (fibers) or pore space component (the index matching fluid), acquisitions of both positive and negative images of the porous fibrous materials are demonstrated. Image analysis techniques are then applied to the 3D reconstructions of the fibrous materials to extract important morphological characteristics such as porosity, specific surface area, distributions of fiber diameter and of pore diameter, and fiber orientation distribution; the results are compared with other experimental measurements where available. The topology of the pore space is quantified for an electrospun mat for the first time using the Euler-Poincaré characteristic. Finally, a method is presented for subdividing the pore space into a network of cavities and the gates that interconnect them, by which the network structure of the pore space in these electrospun mats is determined.

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“…An analysis method that combines a series of algorithms has been developed. The algorithms are applied in series to the images in three dimensions to identify the pores and the interconnects, and then obtain the pore and interconnect size distributions [22,49,71,72]. Initially, the macropores are identified ( figure 6).…”

Section: (D) Characterization Of Macroporous Networkmentioning

confidence: 99%

Characterizing the hierarchical structures of bioactive sol–gel silicate glass and hybrid scaffolds for bone regeneration

Martin

Sheng

Hanna

et al. 2012

Phil. Trans. R. Soc. A.

122

106

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Bone is the second most widely transplanted tissue after blood. Synthetic alternatives are needed that can reduce the need for transplants and regenerate bone by acting as active temporary templates for bone growth. Bioactive glasses are one of the most promising bone replacement/regeneration materials because they bond to existing bone, are degradable and stimulate new bone growth by the action of their dissolution products on cells. Sol-gel-derived bioactive glasses can be foamed to produce interconnected macropores suitable for tissue ingrowth, particularly cell migration and vascularization and cell penetration. The scaffolds fulfil many of the criteria of an ideal synthetic bone graft, but are not suitable for all bone defect sites because they are brittle. One strategy for improving toughness of the scaffolds without losing their other beneficial properties is to synthesize inorganic/organic hybrids. These hybrids have polymers introduced into the sol-gel process so that the organic and inorganic components interact at the molecular level, providing control over mechanical properties and degradation rates. However, a full understanding of how each feature or property of the glass and hybrid scaffolds affects cellular response is needed to optimize the materials and ensure long-term success and clinical products. This review focuses on the techniques that have been developed for characterizing the hierarchical structures of sol-gel glasses and hybrids, from atomicscale amorphous networks, through the covalent bonding between components in hybrids and nanoporosity, to quantifying open macroporous networks of the scaffolds. Methods for non-destructive in situ monitoring of degradation and bioactivity mechanisms of the materials are also included.

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Non-destructive quantitative 3D analysis for the optimisation of tissue scaffolds

Cited by 183 publications

References 35 publications

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Three-dimensional imaging of electrospun fiber mats using confocal laser scanning microscopy and digital image analysis

Characterizing the hierarchical structures of bioactive sol–gel silicate glass and hybrid scaffolds for bone regeneration

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