Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Chang, Hsin‐I; Wang, Yiwei

doi:10.5772/21983

Cited by 331 publications

(340 citation statements)

References 108 publications

(81 reference statements)

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“…Surface charge has been recently described as an important 40 parameter on the cell attachment and proliferation 47 . Previous investigations have demonstrated the influence of the polarization of electroactive PVDF on biological cell response.…”

mentioning

confidence: 99%

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

et al. 2013

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This work reports on the influence of polarization and morphology of electroactive poly(vinylidene fluoride), PVDF, on the biological response of myoblast cells. Non-poled, ''poled +'' and "poled-" -PVDF were prepared in the form of films. Further, random and aligned electrospun -PVDF fiber mats were also prepared. It is demonstrated that negatively charged surfaces improve cell adhesion and proliferation and that the directional growth of the myoblast cells can be achieved by the cell culture on oriented fibers. Therefore, 10 the potential application of electroative materials for muscle regeneration is demonstrated.

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mentioning

confidence: 99%

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

et al. 2013

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“…These cells were smaller than myogenic cells, and the interaction with the topography of the tested scaffold was less noticeable. Material surface roughness has a direct influence on cellular morphology, proliferation and phenotype [66]. For instance, primary rat osteoblasts had higher proliferation, an elevated alkaline phosphatase (ALP) activity and an osteocalcin expression on the rough surface (the min-max span of the height was 0.81 µm) in comparison with a smooth one [67].…”

Section: Cell Growth On 3d Pla Scaffoldsmentioning

confidence: 99%

3D Microporous Scaffolds Manufactured via Combination of Fused Filament Fabrication and Direct Laser Writing Ablation

et al. 2014

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A 3D printing fused filament fabrication (FFF) approach has been implemented for the creation of microstructures having an internal 3D microstructure geometry. These objects were produced without any sacrificial structures or additional support materials, just by precisely tuning the nozzle heating, fan cooling and translation velocity parameters. The manufactured microporous structures out of polylactic acid (PLA) had fully controllable Micromachines 2014, 5 840 porosity (20%-60%) and consisted of desired volume pores (∼0.056 µm 3 ). The prepared scaffolds showed biocompatibility and were suitable for the primary stem cell growth. In addition, direct laser writing (DLW) ablation was employed to modify the surfaces of the PLA structures, drill holes, as well as shape the outer geometries of the created objects. The proposed combination of FFF printing with DLW offers successful fabrication of 3D microporous structures with functionalization capabilities, such as the modification of surfaces, the generation of grooves and microholes and cutting out precisely shaped structures (micro-arrows, micro-gears). The produced structures could serve as biomedical templates for cell culturing, as well as biodegradable implants for tissue engineering. The additional micro-architecture is important in connection with the cell types used for the intention of cell growing. Moreover, we show that surface roughness can be modified at the nanoscale by immersion into an acetone bath, thus increasing the hydrophilicity. The approach is not limited to biomedical applications, it could be employed for the manufacturing of bioresorbable 3D microfluidic and micromechanic structures.

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“…Coral have design and ability to serve microenvironment for living cell [4][5][6]. A biodegradable scaffold is a scaffold which is able to dissolve when exposed to organism body fluid [2]. For bone regeneration, the availability of biodegradable scaffold is required.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, scaffold, signaling molecules and cells are considered three main components in tissue engineering [1,2]. Scaffold guides cells to attach, grow and synthesize extracellular matrix (ECM) and biological molecules [3].…”

Section: Introductionmentioning

confidence: 99%

Degradation profile and fibroblast proliferation on synthetic coral scaffold for bone regeneration

Mahanani¹,

Herningtyas²,

Bachtiar³

et al. 2016

AIP Conference Proceedings

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COLLECTIONS This paper was selected as Featured ARTICLES YOU MAY BE INTERESTED INChitosan-gelatine membrane construct with different cinnamaldehyde concentration as drug delivery system in oral cavity AIP Conference Proceedings 1755, 160006 (2016) 160007 (2016) At the request of all authors of the paper, and with the agreement of the Proceedings Editor, an updated version of this article was published on 27 November 2018. The original version supplied to AIP Publishing included an error in the name of an author, name was listed as Andika Dewi Ana rather than Ika Dewi Ana. This has been corrected in the updated and republished version. Abstract. For bone regeneration, one of the important key factors is the ability of the scaffold to be able to be degraded gradually at the implant side and the ability to provide a microenvironment for cells. No information available yet regarding our new synthetic coral scaffold. The purpose of this study was to observe the degradation profile and the human fibroblast gingiva proliferation on the synthetic coral scaffolds prepared from gelatin and calcium carbonate (CaCO3) in a various concentration of weight% wherein gelatin only (G) was used as a control. Scaffolds were incubated in non-phenol red DMEM for 1, 3, 6, 24, 48, and 72 h, then the supernatant was analyzed by spectrophotometer in 280 nm wavelength. After 72 hours, the degradation rate of the scaffold was accelerated using 1N HCl.The proliferation of human fibroblast gingiva was observed at 24, 48 and 72 hours after seeding 2.10 4 cells/well. A plate of 96 well and DMEM were used in this study. The degradation percentage of the scaffolds within 24 hours was considered small, ranging between 4.23 -5.15%. Meanwhile, the degradation between 24 to 72 hours incubation was confirmed at 38. 35, 62.16, 52.86, 47.86, 48.42, and 53.17% for G, 7/3, 6/4, 5/5, 4/6 and 3/7 respectively. In the accelerated condition, the fastest degradation was found in G.Fibroblast proliferation increased following the incubation time except in control group and 3:7, but no significantly different between the various concentration. The fastest degradation rate was found in the scaffold without CaCO3 addition, confirming that CaCO3 in certain concentration prolonged the half-life of the scaffold. The proliferation of fibroblast is increased for all incubation time. Degradation Profile and Fibroblast Proliferation on Synthetic Coral Scaffold for Bone Regeneration

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Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Cited by 331 publications

References 108 publications

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

3D Microporous Scaffolds Manufactured via Combination of Fused Filament Fabrication and Direct Laser Writing Ablation

Degradation profile and fibroblast proliferation on synthetic coral scaffold for bone regeneration

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