Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics

Hattori, Akira; Kim, Hyonchol; Terazono, Hideyuki; Hayashi, Masahito; Kaneko, Tomoyuki; Nomura, Fumimasa

doi:10.1007/s10404-012-1112-6

Cited by 17 publications

(21 citation statements)

References 59 publications

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“…These four channels connected at the high voltage amplifier deflected droplets of interest into the collection channels by electrophoresis2728. Our method was adapted from agarose gel liquid electrodes19. To reduce the viscosity for filling narrow liquid electrode spaces, we adopted saline instead of agarose gel and used simple disposable syringes (10 mL) to fill them in the narrow channel (20 μm) of thin poly(dimethylsiloxane) (PDMS).…”

Section: Resultsmentioning

confidence: 99%

“…In comparison with conventional electrodes reported in the literature, the advantages of the saline solution in the electrode channels are: ( i ) the absence of contact between the main channel and electrodes1921; ( ii ) conducting direct current (DC) pulses of high voltage (up to 1,500 V) without any damage observable on the electrode44; ( iii ) creating the electrode channel using a conventional chip fabrication protocol45; ( iv ) the cost of saline preparation is cheaper than low melting temperature metal alloy or micro–nanoparticles of metal946; ( v ) setting and filling processes of the four electrodes can be performed in less than 5 min prior to analysis. The main disadvantage of the saline solution liquid electrodes compared with agarose liquid electrodes is the possible formation of microscopic NaCl crystals in the electrode channels in the case of long experiments or drying by Joule heat.…”

Section: Discussionmentioning

confidence: 99%

“…Current imaging microfluidic systems locate a target cell in an image using either a threshold value for intensity or compare the image captured from the video with a standard image192021. Despite the obvious success of imaging microfluidic systems for a wide range of applications, droplet interrogation is mostly restricted to simple morphological information such as area, perimeter, and major/minor axis lengths.…”

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confidence: 99%

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An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

Girault¹,

Kim²,

Arakawa³

et al. 2017

Sci Rep

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A microfluidic on-chip imaging cell sorter has several advantages over conventional cell sorting methods, especially to identify cells with complex morphologies such as clusters. One of the remaining problems is how to efficiently discriminate targets at the species level without labelling. Hence, we developed a label-free microfluidic droplet-sorting system based on image recognition of cells in droplets. To test the applicability of this method, a mixture of two plankton species with different morphologies (Dunaliella tertiolecta and Phaeodactylum tricornutum) were successfully identified and discriminated at a rate of 10 Hz. We also examined the ability to detect the number of objects encapsulated in a droplet. Single cell droplets sorted into collection channels showed 91 ± 4.5% and 90 ± 3.8% accuracy for D. tertiolecta and P. tricornutum, respectively. Because we used image recognition to confirm single cell droplets, we achieved highly accurate single cell sorting. The results indicate that the integrated method of droplet imaging cell sorting can provide a complementary sorting approach capable of isolating single target cells from a mixture of cells with high accuracy without any staining.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

Girault¹,

Kim²,

Arakawa³

et al. 2017

Sci Rep

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“…Information about the cells of interest is obtained optically in a nondestructive and quantitative manner Nomura et al 2011a, b;Yasuda et al 2013). As a step toward achieving a cell sorter system for "imaging biomarkers," we have developed an on-chip high-speed multi-imaging cell-sorting system for labelfree collection of target cells Hattori and Yasuda 2010).…”

Section: On-chip Imaging Cell Sorter For Identification and Separatiomentioning

confidence: 99%

Novel Technology to Assay the Multicellular Network: On-Chip Cellomics Technology

Yasuda

2016

Vascular Engineering

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A series of studies aimed at developing methods and technologies of analyzing epigenetic information in cells and in those networks, as well as that of genetic information, was examined to expand our understanding of how living systems are determined. Technologies of analyzing epigenetic information was developed starting from the twin complementary viewpoints of cell regulation as an "algebraic" system (emphasis on temporal aspects) and as a "geometric" system (emphasis on spatial aspects). Exploiting the combination of latest microfabrication technologies and measurement technologies, which we call on-chip cellomics technology, we can select, control, and reconstruct the environments, interaction of single cells and cell networks from "algebraic" and "geometric" viewpoints. In this chapter, our developed technolgoeis and some results for spatial viewpoint of epigenetic information as a part of a series of cell-networkbased "geometric" studies of celluler systems in our research groups are summarized and reported. The knowlege and technolgies acquired from these viewpoints may lead to the use of cells that fully control practical applications like cell-network-based drug screening and the regeneration of organs from cells.

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“…This leads to deterioration of the imaging quality of the system. Apart from PDMS, other polymer materials like PMMA have also been used in IFC systems [15], [16]. However, PMMA also suffers from similar issues as that of PDMS [17].…”

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confidence: 98%

Imaging Flow Cytometry With Femtosecond Laser-Micromachined Glass Microfluidic Channels

Jagannadh

Mackenzie

Pal

et al. 2015

IEEE J. Select. Topics Quantum Electron.

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Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing properties of cells/particles while they are in flow. In this paper, we demonstrate the integration of fused silica microfluidics fabricated using femtosecond laser machining into optofluidic imaging systems. By using glass for the sample stage of our microscope, we have exploited its superior optical quality for imaging and bio-compatibility. By integrating these glass microfluidic devices into a custom-built bright field microscope, we have been able to image red blood cells in flow with high-throughputs and good fidelity. In addition, we also demonstrate imaging as well as detection of fluorescent beads with these microfluidic devices.Index Terms-Ultra-fast laser inscription, microfluidic/ optofluidic imaging, high-throughput imaging, glass microfluidics.

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Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics

Abstract: We have developed a non-destructive imaging flow cell-sorting system using an ultra-high-speed camera (shutter speed of 1/10

Cited by 17 publications

References 59 publications

An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

Novel Technology to Assay the Multicellular Network: On-Chip Cellomics Technology

Imaging Flow Cytometry With Femtosecond Laser-Micromachined Glass Microfluidic Channels

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