This article aims to test FOS (first-order statistical) in extracting features of embryonated eggs. This test uses the initial step of image processing to get the best input image in feature extraction. The image processing method starts from the image acquisition process, then improves with image preprocessing and segmentation. Image acquisition in this study uses the concept of egg candling in a dark place captured with a smartphone camera. The acquisition results are improved by image preprocessing using gray scaling, image enhancement (by Histogram Equalization), and segmentation of chicken egg image. The segmentation results were extracted using FOS with five parameters: mean, entropy, variance, skewness, and kurtosis. Based on the calculation of these parameters, it is graphed and shows the difference in patterns between fertile and infertile eggs. However, some eggs have a similar pattern, thus affecting the identification process. The identification process used neural networks by the backpropagation method for training and testing. The training results provide an accuracy value of 100% of all training data; however, 80% of the new test data obtained test results at testing. This test is carried out with 100 data, 50 each for training and test data. Based on the test results, which significantly affect the level of accuracy is the feature extraction method. FOS pattern in detecting the fertility of chicken eggs by BP Neural Network is still categorized as low, so it is necessary to improve methods to get maximum results.