Non-cytotoxic effect of green synthesized silver nanoparticles and its antibacterial activity

Senthil, B.; Thiyagarajan, Devasena; Prakash, B. Aditya; Rajasekar, Arvindganth

doi:10.1016/j.jphotobiol.2017.10.010

Cited by 99 publications

(56 citation statements)

References 28 publications

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“…Silver nanoparticles are widely used in the drug carrier, cancer treatment, anti-microbial studies, nanotechnology, biotechnology and biomedical field. Over the past few decades, AgNPs synthesis strategies have focused on the development of eco-friendly and rapid green approach for cost effective synthesis methods [37–41]. Therefore, this reaction pathway satisfies all the conditions of a 100% green chemical process in which plants, fruit, bacteria, yeasts, fungi etc.are used [42, 43].…”

Section: Discussionmentioning

confidence: 99%

Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

Erdoğan¹,

Abbak²,

Demirbolat³

et al. 2019

PLoS ONE

229

120

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In this study, we report on the synthesis of silver nanoparticles (AgNPs) from the leaf extracts of Cynara scolymus (Artichoke) using microwave irradiation and the evaluation of its anti-cancer potential with photodynamic therapy (PDT). Silver nanoparticles formation was characterized by scanning electron microscopy with energy dispersive x-ray spectroscopy and Fourier transform infrared (FTIR) spectroscopy. Silver nanoparticles formation was also investigated the surface charge, particle size and distribution using zetasizer analysis. The cytotoxic effect of AgNPs and/or PDT was studied by MTT assay and migration by the scratch assay. The apoptotic inducing ability of the AgNPs and/or PDT was investigated by intracellular ROS analysis, antioxidant enzyme levels (SOD, CAT, GPx and GSH), Hoechst staining and Bax/Bcl-2 analysis using western blotting. The mean particle size of produced AgNPs was found 98.47±2.04 nm with low polydispersity (0.301±0.033). Zeta potential values of AgNPs show -32.3± 0.8 mV. These results clearly indicate the successful formation of AgNPs for cellular uptake. Mitochondrial damage and intracellular ROS production were observed upon treatment with AgNPs (10μg/mL) and PDT (0.5 mJ/cm 2 ) showed significant reducing cell migration, expression of Bax and suppression of Bcl-2. Significantly, biosynthesized AgNPs showed a broad-spectrum anti-cancer activity with PDT therapy and therefore represent promoting ROS generation by modulating mitochondrial apoptosis induction in MCF7 breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

Erdoğan¹,

Abbak²,

Demirbolat³

et al. 2019

PLoS ONE

229

120

View full text Add to dashboard Cite

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“…71 Both of these cell lines have been widely used for cytotoxicity tests of nanoparticles. 70,[72][73][74] The PVA coated Se NPs exhibited no obvious cytotoxicity at concentrations up to 31.2 µg/mL. The viability of Balb/3T3 mouse embryo fibroblasts did decrease somewhat with increasing concentrations of Se NPs, but their viability was not below 70% even at the highest dose of 31 µg/mL (one-sample t-test, p=0.41).…”

Section: Cytotoxicity Test Of Selenium Nanoparticles Using Fibroblastmentioning

confidence: 97%

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Huang¹,

Kumari²,

Herold³

et al. 2020

IJN

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Purpose: Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Methods: Se NPs were coated with a positively charged proteinrecombinant spider silk protein eADF4(κ16)to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4(κ16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Results: Compared to the negatively charged polyvinyl alcohol coated Se NPs, the positively charged eADF4(κ16)-coated Se NPs demonstrated a much higher bactericidal efficacy against the Gram-negative bacteria E. coli, with a minimum bactericidal concentration (MBC) approximately 50 times lower than that of negatively charged Se NPs. Cytotoxicity testing showed that the eADF4(κ16)-coated Se NPs are safe to both Balb/3T3 mouse embryo fibroblasts and HaCaT human skin keratinocytes up to 31 µg/mL, which is much higher than the MBC of these particles against E. coli (8 ± 1 µg/mL). In addition, antibacterial coatings were created by immobilising the eADF4(κ16)-coated Se NPs on positively charged spider silk films and these were shown to retain good bactericidal efficacy and overcome the issue of low particle stability in culture broth. It was found that these Se NPs needed to be released from the film surface in order to exert their antibacterial effects and this release can be regulated by the surface charge of the film, such as the change of the spider silk protein used. Conclusion: Overall, eADF4(κ16)-coated Se NPs are promising new antibacterial agents against life-threatening bacteria.

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“…One of these mechanisms is represented by the induction of apoptosis-related genes and the activation of apoptosis mechanism. Also, it was proven that nanosilver can cause the formation and intracellular accumulation of ROS, modification of mitochondrial membrane permeability, and DNA damage [ 247 , 248 , 249 ]. The in vitro toxicity of AgNPs was investigated in several research studies, but there is still a lack of consistent and reliable data.…”

Section: Toxicity Of Silver Nanoparticlesmentioning

confidence: 99%

Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview

et al. 2018

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During the past few years, silver nanoparticles (AgNPs) became one of the most investigated and explored nanotechnology-derived nanostructures, given the fact that nanosilver-based materials proved to have interesting, challenging, and promising characteristics suitable for various biomedical applications. Among modern biomedical potential of AgNPs, tremendous interest is oriented toward the therapeutically enhanced personalized healthcare practice. AgNPs proved to have genuine features and impressive potential for the development of novel antimicrobial agents, drug-delivery formulations, detection and diagnosis platforms, biomaterial and medical device coatings, tissue restoration and regeneration materials, complex healthcare condition strategies, and performance-enhanced therapeutic alternatives. Given the impressive biomedical-related potential applications of AgNPs, impressive efforts were undertaken on understanding the intricate mechanisms of their biological interactions and possible toxic effects. Within this review, we focused on the latest data regarding the biomedical use of AgNP-based nanostructures, including aspects related to their potential toxicity, unique physiochemical properties, and biofunctional behaviors, discussing herein the intrinsic anti-inflammatory, antibacterial, antiviral, and antifungal activities of silver-based nanostructures.

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Non-cytotoxic effect of green synthesized silver nanoparticles and its antibacterial activity

Cited by 99 publications

References 28 publications

Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview

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