Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

Erdoğan, Ömer; Abbak, Mürüvvet; Demirbolat, Gülen Melike; Birtekocak, Fatih; Aksel, Mehran; Paşa, Salih; Çevik, Özge

doi:10.1371/journal.pone.0216496

Cited by 229 publications

(132 citation statements)

References 53 publications

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“…Induction of cytotoxicity of nanoparticles depends not only on their shape, surface chemistry, or size but also on the cell line studied. Erdogan et al, 21 reported that green silver nanoparticles induced toxicity and showed anti-cancer effects with phototherapy in MCF7 cells.…”

Section: Introductionmentioning

confidence: 99%

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

Bin-Jumah¹,

Al-Abdan²,

Albasher³

et al. 2020

IJN

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Introduction: Extensive use of metallic nanomaterials in different areas of agriculture and commercial products induce significant harmful effects on human health and the environment. In the current study, we synthesized an eco-friendly approach silver nanoparticles (AgNPs) using root extracts of Beta vulgaris L. Methods: The synthesized green silver nanoparticles (gAgNPs) were characterized by dynamic light scattering (DLS) and high-resolution transmission electron microscope (HR-TEM). The gAgNPs had a round shape and the mean size was 20−50 nm. The cytotoxic effects of gAgNPs were determined in human hepatic normal (CHANG) and cancer (HUH-7) cells by using tetrazolium salt (MTT) and lactate dehydrogenase (LDH) assays for 24 h. Results and Discussion: It was clear from the observations of this experiment that higher concentrations of gAgNPs reduce cell viability. The production of reactive oxygen species (ROS) was evaluated by using DCFDA. The gAgNPs induced more ROS in the HuH-7 cells than in the CHANG cells. The fragmentation of DNA was evaluated by alkaline single-cell gel electrophoresis and the maximum DNA strand breakage was found at a higher concentration exposure of gAgNPs for 24 h. It is important to notice that the HuH-7 cells showed an increased sensitivity to gAgNPs than the CHANG cells. The apoptotic and necrotic effects of gAgNPs on both the cells were evaluated using annexin-V-FITC and propidium iodide staining. An increased count of apoptotic and necrotic cells was found following a higher concentration exposure of gAgNPs. The apoptotic protein expression in these cells due to gAgNPs exposure was determined using immunoblotting techniques and the level of Bcl2 was decreased. However, the expression of BAX and protein was increased in both cells. Conclusion: Therefore, it can be concluded that higher concentrations of gAgNPs may induce significant cytotoxicity and cause DNA damage and apoptosis.

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Section: Introductionmentioning

confidence: 99%

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

Bin-Jumah¹,

Al-Abdan²,

Albasher³

et al. 2020

IJN

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“…In order to determine the cells levels of caspase-3 activity, cells were lysed with cell lysis buffer and centrifuged for 10 min at 9000 rpm at 4˚C after treatment of Anti-miR-144 (20 nM) for 36 h (Erdogan et al, 2019). Using a commercial kit (Calbiochem, USA) and following the manufacturer's instructions, the supernatant was used for measuring caspase-3 activity as a marker of apoptosis in cells.…”

Section: Caspase-3 Activity Assaymentioning

confidence: 99%

Suppression of MicroRNA-144 Promotes CXCR4 and CXCL12 Expression and Downregulates Apoptosis in Ovarian Cancer Cells

Turut

Acıdereli

Çevik

2020

Preprint

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MicroRNAs are important regulators in the growth and metastasis of ovarian cancers. Many assays were established to identify the role of miR-144-3p in ovarian cancer cells and its interaction with COX-2 and chemokines (CXCR4 and CXCL12). The ovarian cancer cells (OVCAR-3 and SKOV-3) were transfected with Anti-miR-144 to downregulate the miR-144-3p and cultured for 36 h. We herein examined the cell viability, colony formation, cell migration, COX-2 reporter activity, the protein expressions of CXCR4, CXCL12, COX-2, VEGF, Caspase-3, BAX and Bcl-2. We have observed that the suppression of miR-144-3p significantly increased the cell proliferation and migration and decreased the apoptosis. Moreover, the downregulation of miR-144-3p markedly increased the COX-2, CXCR4, CXCL12 and VEGF expression in OVCAR-3 and SKOV-3 ovarian cancer cells. In conclusion, miR-144-3p may play important roles in the regulation of chemokine receptor CXCR4 and its ligand CXCL12 in the progressive ovarian tumors expressing COX2. These data suggests that miR-144 has the novel therapeutic targets for the cancer therapy and cancer prevention.

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“…An organic methodology has been utilized in the synthesis of AgNPs utilizing microorganisms, fungi and plant extract prompting to reliable alterative to chemical and physical techniques in acquiring the nanoparticles in controlled particle size. It has been seen that green synthesis of AgNPs with various stabilizing agents, for example, polyethylene glycol, alcohol vinyl, dextran, cyclodextrins and utilizing DOI: http://dx.doi.org/10.5772/intechopen.90717 "andeli" (rose extract) [19][20][21][22]. This expands utilization of AgNPs in different materials has prompted a more straightforward and direct exposure in human and raised potential dangers to health issues.…”

Section: Toxicity Of Silver Nanoparticlesmentioning

confidence: 99%

Cellular and Molecular Impact of Green Synthesized Silver Nanoparticles

Patel¹,

Kumari²,

Verma³

et al. 2020

Engineered Nanomaterials - Health and Safety

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Toxicity and biocompatibility of silver nanoparticles are of a major concern due to their extensive production regardless of their application in current industries. Information about toxicology or biocompatibility is crucial regarding their proper utilization and application in clinical as well as environmental aspect. This chapter describes in detail about the different techniques and technology of synthesis of silver nanoparticles and explains their different physiochemical properties in context of the current research scenario. Further, it also explains the biocompatibility and toxicity of silver nanoparticles at cellular and molecular aspects. The mechanism of their toxicity has been described keeping in view of the recent research done. In brief, it reveals detail knowledge of the cellular and molecular impact of silver nanoparticles.

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Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells

Cited by 229 publications

References 53 publications

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

Suppression of MicroRNA-144 Promotes CXCR4 and CXCL12 Expression and Downregulates Apoptosis in Ovarian Cancer Cells

Cellular and Molecular Impact of Green Synthesized Silver Nanoparticles

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