Non-covalent labeling of human serum albumin with indocyanine green: a study by capillary electrophoresis with diode laser-induced fluorescence detection

Moody, Elizabeth; Viskari, Pertti J.; Colyer, Christa L.

doi:10.1016/s0378-4347(99)00121-8

Cited by 98 publications

(85 citation statements)

References 23 publications

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“…The ICG fluorescence quantum yield ( F ) changes when the dye is adsorbed to macromolecules such as proteins and enzymes. In its non-bonded state, ICG is only weakly fluorescent, 20 which we observed in our NP protocols. The fluorescence quantum yields of the monomer at low concentration are 0.04 ± 0.004 in methanol and 0.03 ± 0.006 in NP.…”

Section: Figmentioning

confidence: 52%

Indocyanine Green Nanoparticles Useful for Photomedicine

Gomes

Lunardi²,

Marchetti³

et al. 2006

Photomedicine and Laser Surgery

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Objective: The aim of this study was to evaluate the potential application of biodegradable nanoparticles (NPs) containing indocyanine green (ICG) in photodynamic therapy (PDT). Methods: Important parameters, such as particle size and external morphology, were established by dynamic light scattering (DLS) and scanning electron microscopy (SEM). Also, drug encapsulation efficiency and in vitro release behavior were evaluated by spectroscopic methods. Results: The particles are spherical in shape, they exhibit an 817-nm diameter, and they have a low tendency to aggregate. The loading efficiency was 65%. ICG photophysical parameters showed a bathocromic shift in ICG-loaded nanoparticles (ICG-NP). Analysis of the cell P388-D1 in the presence of the ICG-NP by SEM showed that the majority of the nanoparticles were uptaken by phagocytic cells after 2 h of incubation. After laser irradiation photodamage was observed in P388-D1 cells where ICG-NPs had been uptaken by phagocytic cells. Conclusion: Polymeric NPs work as an efficient drug delivery system for PDT drugs, and this approach can be used in the administration of amphiphilic photosensitizers in the treatment of neoplasic cells. 514

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Section: Figmentioning

confidence: 52%

Indocyanine Green Nanoparticles Useful for Photomedicine

Gomes

Lunardi²,

Marchetti³

et al. 2006

Photomedicine and Laser Surgery

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“…Polycationic cyanine derivatives including 9-11 have been synthesized for a strong binding to nucleic acids [19][20][21][22][23][24][25]. On the other hand, dyes 12-14 with a net charge of -1 show strong interaction with proteins [26][27][28][29][30]. The binding studies are discussed in more detail as follows.…”

Section: Cyanine Dyesmentioning

confidence: 99%

“…The use of indocyanine green (12 in Scheme 3) for determination of human serum albumin (HSA) [26,27] and a number of other proteins [27] by capillary electrophoresis with diode laser-induced fluorescence detection (CE-LIF) has been investigated recently. This negatively charged dye shows a strong absorption at 780 nm and a weak fluorescence at 820 nm in aqueous solution.…”

Section: Cyanine Dyesmentioning

confidence: 99%

Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes

et al. 2004

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Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.

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“…To further address this potential, we also show the first results of an ongoing clinical pilot study where the ICG99m Tc-NanoColl cocktail was used in six patients with prostate carcinoma. To confirm the in-situ formation of self-assembled multimodal complexes, 28 protein separations were performed on the albumin particle containing cocktails in micron centrifugal filters (Millipore, Billerica, Massachusetts) with a molecular weight cut of 30,000 Da. Solutions were centrifuged for 8 min at 7×10 3 rpm and the fluorescent content of the residue and filtrate was determined.…”

Section: Introductionmentioning

confidence: 99%

Tracer-cocktail injections for combined pre- and intraoperative multimodal imaging of lymph nodes in a spontaneous mouse prostate tumor model

et al. 2011

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Abstract. To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre-and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: 1. a cocktail of patent blue (Pb) and indocyanine green (ICG); 2. a cocktail of albumin radiocolloids ( 99m TcNanoColl), Pb, and ICG; or 3. a cocktail of radiolabeled albumin ( 99m Tc-Vasculosis), Pb, and ICG. The distribution of these imaging agents over the lymph nodes (LNs) are studied at different time points after injection. We find that at 60-min postinjection, ICG significantly improves the detection of the LNs compared to Pb, 53 versus 7%, respectively. Moreover, a cocktail of ICG and 99m Tc-NanoColl improves the fluorescent detection rate to 86%, equalling that of the clinically applied 99m Tc-NanoColl. A similar overlap is observed in our initial clinical pilot data. Fluorescent detection of the LNs using a ICG with 99m Tc-Vasculosis gives similar results as "free" ICG (58%; 60 min). A 99m Tc-NanoColl, Pb, and cocktail ICG enriches the standard 99m Tc-NanoColl approach by adding optical detection of the sentinel lymph nodes. Furthermore, this approach improves fluorescent-based guidance and enables both accurate surgical planning and intraoperative detection, based on a single injection. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Non-covalent labeling of human serum albumin with indocyanine green: a study by capillary electrophoresis with diode laser-induced fluorescence detection

Cited by 98 publications

References 23 publications

Indocyanine Green Nanoparticles Useful for Photomedicine

Indocyanine Green Nanoparticles Useful for Photomedicine

Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes

Tracer-cocktail injections for combined pre- and intraoperative multimodal imaging of lymph nodes in a spontaneous mouse prostate tumor model

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