Non-contact scanning diffuse correlation tomography system for three-dimensional blood flow imaging in a murine bone graft model

Abstract

Abstract:A non-contact galvanometer-based optical scanning system for diffuse correlation tomography was developed for monitoring bone graft healing in a murine femur model. A linear image reconstruction algorithm for diffuse correlation tomography was tested using finite-element method based simulated data and experimental data from a femur or a tube suspended in a homogeneous liquid phantom. Finally, the non-contact system was utilized to monitor in vivo blood flow changes prior to and one week after bone graft transplantation within murine femurs. Localized blood flow changes were observed in three mice, demonstrating a potential for quantification of longitudinal blood flow associated with bone graft healing.

Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy

“…This limitation resulted in the lack of blood flow information during the tumor growth prior to treatment. To address these limitations, a non-contact scanning diffuse correlation tomography system [72,73] will be adapted for animal cancer monitoring. This will enable measurements of tumors at any time during their development and provide reliable three-dimensional blood flow imaging with spatially dense datasets from the scanning system.…”

In Vivo, Non-Invasive Characterization of Human Bone by Hybrid Broadband (600-1200 nm) Diffuse Optical and Correlation Spectroscopies

“…Collagen can be assessed non-invasively by diffuse optical techniques. High penetration depth along with the non-invasive nature of diffuse optical methods has found various applications in in vivo diagnostics: tissue characterization [10], hemodynamics monitoring [11,12,13], brain oximetry [11,14], and optical mammography [15,16,17,18] are few examples where the application of the technique has been explored deeply. Prior attempts to characterize bone tissue by diffuse optical techniques lack either the broadband nature [19,20] or number of subjects [21].…”

“…Importantly, a broadband TD system can extract the concentration of in vivo deep tissue constituents like collagen, lipid, water, hemodynamic parameters like oxy-, deoxy-hemoglobin, and oxygen saturation, making TD a promising technique to complement DEXA, which is insensitive to the organic part of bone tissue. Furthermore, Diffuse Correlation Spectroscopy, a non-invasive technique that allows the absolute estimate of the blood flow by optical means, at depths comparable with diffuse optical spectroscopy (few cm), has been shown in some studies to be able to measure the micro perfusion of bone [12,19]. …”

Longitudinal 3D Blood Flow Distribution Provided by Diffuse Correlation Tomography during Bone Healing in a Murine Fracture Model

“…In our laboratory, a noncontact DCT technique has been developed for assessing blood flow in a murine femoral graft model. The DCT technique has been verified and applied in previous tissue phantom and longitudinal bone graft monitoring studies . In our weekly bone graft measurements, blood flow in grafts increased at one week after graft surgery.…”