Non-coding RNAs in lung cancer

Ricciuti, Biagio; Mecca, Carmen; Crinò, Lucio; Baglivo, Sara; Cenci, Matteo; Metro, Giulio

doi:10.18632/oncoscience.98

Cited by 32 publications

(43 citation statements)

References 315 publications

(324 reference statements)

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“…miRNAs are reported to be involved in both physiological and pathological processes of pantosomatous organs. Multiple miRNAs that may relate to cancer have been discovered, and numerous studies have demonstrated that several miRNAs are up-regulated (miR-21, miR-17, miR-221) or down-regulated (miR-15, miR-34a, miR-140) in lung cancer in particular [12-14]. Several studies have reported differential expression of miRNAs in the development of NSCLC [13].…”

Section: Introductionmentioning

confidence: 99%

MiR-140 Expression Regulates Cell Proliferation and Targets PD-L1 in NSCLC

Xie

Liang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Programmed death ligand1(PD-L1) plays a role in the development and progression of non-small cell lung cancer (NSCLC). This study aimed to identify miRNA(s) that are responsible for regulation of expression of PD-L1 in NSCLC, and to investigate the role of PD-L1 in regulation of the cell cycle in NSCLC. Methods: We predicted the target miRNA of PD-L1, which was miR-140, using the online tools TargetScan and miBase. In NSCLC cells obtained from clinical specimens, in addition to A549 and NCI-H1650 cell cultures, western blots were used to detect the level of expression of proteins, while real-time PCR was used to determine the level of expression of PD-L1, miR-140, cyclin E, and β-actin. Transfection with miR-140 mimics, miR-140 inhibitors, and PD-L1 siRNA were conducted using commercial kits. To determine whether miR-140 directly binds PD-L1, a luciferase reporter gene with wild type or mutated PD-L1 was used. Cell viability was measured with the MTT assay, and PI staining was used for cell cycle analysis. Results: We found low expression of miR-140 and high expression of PD-L1 and cyclin E in NSCLC cells. Over-expression of miR-140 suppressed the expression of PD-L1 by directly binding its 3’ UTR, and was also associated with decreased expression of cyclin E and inhibition of cellular proliferation in A549 and NCI-H1650 cells. Inhibition of PD-L1, in the absence of manipulations to miR-140, also decreased the expression of cyclin E. Conclusion: We conclude that miR-140 directly suppresses PD-L1 and inhibits the miR-140/PD-L1/cyclin E pathway in NSCLC.

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Section: Introductionmentioning

confidence: 99%

MiR-140 Expression Regulates Cell Proliferation and Targets PD-L1 in NSCLC

Xie

Liang

et al. 2018

Cell Physiol Biochem

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“…Lung cancer is currently the leading cause of death worldwide and 80% of patients are diagnosed as nonsmall cell lung cancer (NSCLC) [1,2]. The poor prognosis with a 5-year survival rate of 16% is due not only to the late stage diagnosis but also to the lack of effective treatments [3,4].…”

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confidence: 99%

“…Human microRNA genes are frequently located at cancer-associated genomic regions or in fragile sites [8]. In recent studies, several miRNAs such as miR-145, miR-186 and the let-7 family were considered suppressors for the metastasis and epithelial mesenchymal transition (EMT) in lung cancer [2,9,10] while miR-10b and miR-155 were positively correlated with the NSCLC tumorous process [11,12]. Therefore, there is a need to investigate the specific miRNAs as putative indicators for NSCLC metastasis more comprehensively and provide a sensitive method for the early diagnosis of NSCLC.…”

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confidence: 99%

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Xu¹,

Li²,

Li³

et al. 2015

Aging and disease

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Lymph node (LN) metastasis is often an early event in the progression of malignant tumors and it contributes to the majority of cancer mortalities. MiRNAs play key roles in tumor metastasis. This study aimed to investigate the specific miRNAs as putative indicators of metastasis early diagnosis for non-small cell lung cancer (NSCLC). In this study, five NSCLC cases with LN metastasis and four cases without metastasis (NLN) were enrolled for Agilent Human miRNA array. The interested differentially expressed miRNA was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the LN metastasis (n = 46) and NLN (n = 39) groups. The microarray results revealed that three miRNAs (miR-1260b, miR-423-3p, miR-23a-5p) were differentially expressed in LN metastasis group compared with NLN group. The expression of miR-1260b was tested by qRT-PCR and the mean relative expression fold change (2 -ΔΔCt ) in LN metastasis was significantly higher than that in the NLN group (3.942, 1.743 respectively, P = 1.179E-04). The patients with tumor-node-metastasis (TNM) stage III were identified more frequently in LN metastasis group (P = 1.772E-11) and with a higher expression level of miR-1260b (5.126, P = 1.147E-06). In addition, the LN metastasis cases were associated with a poorly differentiated degree (P = 0.007). The overexpression of miR1260b in NSCLC with LN metastasis can be regarded as a specific signature for early progression and prognosis of NSCLC.

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“…Moreover, miRNAs in our MO‐BNs act as master regulators of biophysical pathways. For instance, mir‐20a can impact both RP2 and LC signaling, where it is involved in lung cancer progression through oncogenic processes like cellular proliferation, and apoptosis and it was also shown in Ref. to sustain T cell response in favor of an antitumor activity impacting cytokines changes (e.g., IP‐10), which are associated with higher grade toxicities as could be inferred from our network.…”

Section: Discussionmentioning

confidence: 56%