Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Abstract

Lymph node (LN) metastasis is often an early event in the progression of malignant tumors and it contributes to the majority of cancer mortalities. MiRNAs play key roles in tumor metastasis. This study aimed to investigate the specific miRNAs as putative indicators of metastasis early diagnosis for non-small cell lung cancer (NSCLC). In this study, five NSCLC cases with LN metastasis and four cases without metastasis (NLN) were enrolled for Agilent Human miRNA array. The interested differentially expressed miRNA was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the LN metastasis (n = 46) and NLN (n = 39) groups. The microarray results revealed that three miRNAs (miR-1260b, miR-423-3p, miR-23a-5p) were differentially expressed in LN metastasis group compared with NLN group. The expression of miR-1260b was tested by qRT-PCR and the mean relative expression fold change (2 -ΔΔCt ) in LN metastasis was significantly higher than that in the NLN group (3.942, 1.743 respectively, P = 1.179E-04). The patients with tumor-node-metastasis (TNM) stage III were identified more frequently in LN metastasis group (P = 1.772E-11) and with a higher expression level of miR-1260b (5.126, P = 1.147E-06). In addition, the LN metastasis cases were associated with a poorly differentiated degree (P = 0.007). The overexpression of miR1260b in NSCLC with LN metastasis can be regarded as a specific signature for early progression and prognosis of NSCLC.

Epstein-Barr Virus Infection of Cell Lines Derived from Diffuse Large B-Cell Lymphomas Alters MicroRNA Loading of the Ago2 Complex

“…We observed an almost complete loss of miR-1260 from the Ago complexes in the four cell lines, in line with its association with Ago1 (41). This miRNA was shown to differentiate between GCB-and ABC-DLBCLs (42) and is considered to have oncogenic properties (43). We assume that the enrichment of growth-retarding miRNAs like miR-320a to a level only slightly above the 0.1% cutoff is offset by a rise of growth-promoting miRNAs, either by their overall level or by their Ago2 enrichment.…”

Oncogenic miR-23a-5p is associated with cellular function in RCC

“…In this study, we found that the expression of miR-23a-5p in RCC tissues is obviously higher than the expression in paired normal tissues. The result also happened on HCC and NSCLC (9,10). Meanwhile, upregulation of miR-23a-5p could promote the proliferation, migration and invasion in RCC cell lines while downregulation of miR-23a-5p played an inhibitory role in RCC cell lines.…”

“…miR-23a-5p was located at chromosome 19 and recently involved in various types of cancers, including hepatocellular carcinoma (9), non-small cell lung cancer (NSCLC) (10) and so on. But there is no study about miR-23a-5p in RCC.…”

Effects of β-carotene on Expression of Selected MicroRNAs, Histone Acetylation, and DNA Methylation in Colon Cancer Stem Cells

“…For example, miRNA-1260b targets Wnt antagonist genes and inhibits tumor suppressor genes in renal cancer [37]. In addition, overexpression of miRNA-1260b has been reported to be involved in the metastasis of non-small cell lung cancer [38]. For miR-296-3p, it has exhibited resistance to natural killer cells to increase metastatic potential of prostate cancer cells [39].…”