Nocardiopsis sediminis sp. nov., isolated from mangrove sediment

Abstract

A filamentous actinomycete, designated strain 1SS5-02T, was isolated from mangrove sediment collected from Ranong province, Thailand. The strain formed aerial and substrate mycelia composed of long, branched hyphae. Aerial mycelia differentiated into non-motile, rod-shaped spores. The organism contained meso-diaminopimelic acid and no diagnostic sugars in whole-cell hydrolysates. The predominant menaquinones were MK-11(H4), MK-11(H6) and MK-11(H8). Polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. The major fatty acids were iso-C16 : 0, C18 : 1ω9c, 10-methyl C18 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 73.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1SS5-02T belonged to the genus Nocardiopsis. The strain showed the highest degree of 16S rRNA gene sequence similarity with 'Nocardiopsis mangrovei' HA11166 (97.9 %) and Nocardiopsis trehalosi VKM Ac-942T (97.8 %). However, strain 1SS5-02T could be distinguished from its nearest phylogenetic relatives in the genus Nocardiopsis on the basis of DNA-DNA relatedness values and the combination of phenotypic properties. On the basis of polyphasic taxonomy, strain 1SS5-02T is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis sediminis sp. nov. is proposed. The type strain is 1SS5-02T (=BCC 75410T=NBRC 110934T).

Genome-Based Taxonomic Classification of the Phylum Actinobacteria

“…Basonym: V errucosispora sediminis Dai et al 2010 (The name Micromonospora sediminis has already been validly published (Muangham et al, 2016 ), hence a new epithet has been chosen to avoid homonyms).…”

Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087T Under Salt Stress

“…Members of this genus are widely distributed in hypersaline environments and produce very distinct secondary metabolites ( Tsujibo et al, 2003 ; Sun et al, 2015 , 2017 ; Sharma and Singh, 2016 ). In past few years, attempts to isolate novel Nocardiopsis strains were made and many novel species such as N. algeriensis ( Bouras et al, 2015 ), N. mangrovei ( Huang et al, 2015 ), N. oceani and N. nanhaiensis ( Pan et al, 2015 ), N. ansamitocini ( Zhang et al, 2016b ), N. sediminis ( Muangham et al, 2016 ), N. rhizosphaerae ( Zhang et al, 2016c ), and N. akesuensis ( Gao et al, 2016 ) have been reported. At present, the genus comprises 53 species and 5 subspecies ( 1 2017).…”

Nocardiopsis coralli sp. nov. a novel actinobacterium isolated from the coral Galaxea astreata

“…The members of the genus Nocardiopsis were widely distributed in the environment, mainly in soil, especially alkaline soil [3], desert [4, 5] and high-salinity soil [6, 7]. This genus encompasses distinct features from other genera, such as well-developed substrate mycelium that fragments into non-motile bacillary and coccoid elements [8] and aerial hyphae that differentiates into smooth surfaced spores; whole cell hydrolysates contain meso -diaminopimelic acid but lack diagnostic sugars [9]; the major menaquinones comprise variably hydrogenated menaquinones with ten isoprene units [10]; the predominant polar lipids include phosphatidylethanolamine and phosphatidylglycerol [11]. Species of the genus Nocardiopsis were characterized chemotaxonomically and physiologically in their taxonomy, and distinguished between Nocardiopsis species by differences in cell wall composition and menaquinone, phospholipid, and fatty acid patterns [12].…”