Nocardiopsis nikkonensis sp. nov., isolated from a compost sample

Abstract

An actinomycete strain, designated YU1183-22 T , was isolated from a compost sample collected in Nikko, Japan. The isolate formed white aerial mycelium with relatively long aerial hyphae showing chains of arthrospores. Strain YU1183-22 T grew with 0-10 % (w/v) NaCl, at pH 6-11 and at 10-37 6C (optimum 30 6C). Strain YU1183-22 T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H 10 ) and ). The polar lipids were phosphatidylcholine and phosphatidylglycerol. The major cellular fatty acids were iso-C 16 : 0 , anteiso-C 17 : 0 and tuberculostearic acid. The G+C content of the DNA was 72.3 mol%. Chemotaxonomic and morphological characterization clearly demonstrated that strain YU1183-22 T belonged to the genus Nocardiopsis. Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to Nocardiopsis salina YIM 90010 T (98.0 % 16S rRNA gene sequence similarity), Nocardiopsis xinjiangensis YIM 90004 T (97.9 %) and Nocardiopsis kunsanensis HA-9 T (97.3 %). However, DNA-DNA relatedness as well as physiological and biochemical analyses showed that strain YU1183-22 T could be differentiated from its closest phylogenetic relatives. It is proposed that this strain be classified as a representative of a novel species of the genus Nocardiopsis, with the name Nocardiopsis nikkonensis sp. nov. The type strain is YU1183-22 T (5NBRC 102170 T 5KCTC 19666 T ).The genus Nocardiopsis is a member of the family Nocardiopsaceae (Meyer, 1976) and, at the time of writing, about 30 species have been described, including Nocardiopsis arabia, N. valliformis, N. quinghaiensis and N. ganjiahuensis (Hozzein & Goodfellow, 2008;Yang et al., 2008;Chen et al., 2008;Zhang et al., 2008). Many members of the genus Nocardiopsis have been isolated from hypersaline or alkaline environments. However, others have been isolated from non-hypersaline environments, including the sputum of a kidney transplant patient, the rhizosphere of Casuarina sp. and the atmosphere of a composting facility (Yassin et al., 1997; Evtushenko et al., 2000; Kämpfer et al., 2002). Nocardiopsis species are of value to industry as they are known to produce bioactive agents such as griseusin D, apoptolidin and methylpendolmycin (Li et al., 2007;Kim et al., 1997;Sun et al., 1991). Hence, the discovery of additional species of this genus will contribute both to an understanding of their ecological roles and to the provision of bioresources for industrial applications.Strain YU1183-22 T was isolated from a matured compost sample collected in Nikko, Japan, by growth on HV agar (Hayakawa & Nonomura, 1987) containing (l 21 ) 20 mg nalidixic acid, 50 mg cycloheximide and 100 g NaCl. After incubation at 28 u C for 14 days, colonies of strain YU1183-22 T showed a thin, flat morphology with sparse, white, aerial hyphae.Morphological features of cells of strain YU1183-22 T grown on HV agar and oatmeal-YGG agar (Hayakawa et al., 1982) at 28 u C for 14 days were analysed by light and scanning electron microsc...

A Combination of Biochar–Mineral Complexes and Compost Improves Soil Bacterial Processes, Soil Quality, and Plant Properties

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Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

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Bioactive Metabolite Production by Nocardiopsis Synnemataformansvls-10 of Mangrove Origin

“…The requirement of sodium chloride for production of bioactive metabolites seems to be different among actinobacterial strains. Optimum sodium chloride concentration for antimicrobial metabolite production was reported to be 9% for Nocardiopsis nikkonensis [71] and Nocardiopsis xinjiangensis [72].…”