Nocardiopsis and Saccharothrix genera in Saharan soils in Algeria: Isolation, biological activities and partial characterization of antibiotics

Zitouni, Abdelghani; Boudjella, Hadjira; Lamari, Lynda; Badji, Boubekeur; Mathieu, Florence; Lebrihi, Ahmed; Sabaou, Nasserdine

doi:10.1016/j.resmic.2005.05.006

Cited by 62 publications

(55 citation statements)

References 30 publications

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“…The six actinomycete strains were selected on ISP-2 medium after 10 days at 28 uC, mainly based on the results from Verheecke et al (2014): antagonism on contact with A. flavus, reduction of AFs concentration under 17 % versus control and growth on ISP-2 medium (unpublished data). Their 16S rRNA genes were sequenced according to the method described by Zitouni et al (2005). The six strains were identified as Streptomyces roseolus S06, Streptomyces calvus S13, Streptomyces thinghirensis S17, Streptomyces sp.…”

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confidence: 99%

Reduction of aflatoxin production by Aspergillus flavus and Aspergillus parasiticus in interaction with Streptomyces

et al. 2015

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The aim of this study is to investigate aflatoxin gene expression during Streptomyces-Aspergillus interaction. Aflatoxins are carcinogenic compounds produced mainly by Aspergillus flavus and Aspergillus parasiticus. A previous study has shown that Streptomyces-A. flavus interaction can reduce aflatoxin content in vitro. Here, we first validated this same effect in the interaction with A. parasiticus. Moreover, we showed that growth reduction and aflatoxin content were correlated in A. parasiticus but not in A. flavus. Secondly, we investigated the mechanisms of action by reversetranscriptase quantitative PCR. As microbial interaction can lead to variations in expression of household genes, the most stable [act1, btub (and cox5 for A. parasiticus)] were chosen using geNorm software. To shed light on the mechanisms involved, we studied during the interaction the expression of five genes (aflD, aflM, aflP, aflR and aflS). Overall, the results of aflatoxin gene expression showed that Streptomyces repressed gene expression to a greater level in A. parasiticus than in A. flavus. Expression of aflR and aflS was generally repressed in both Aspergillus species. Expression of aflM was repressed and was correlated with aflatoxin B1 content. The results suggest that aflM expression could be a potential aflatoxin indicator in Streptomyces species interactions. Therefore, we demonstrate that Streptomyces can reduce aflatoxin production by both Aspergillus species and that this effect can be correlated with the repression of aflM expression. INTRODUCTIONAflatoxins (AFs) are polyketide-derived furanocoumarins. They are produced by fungi of the genus Aspergillus (including Aspergillus flavus and Aspergillus parasiticus) in agricultural foodstuffs (maize, hazelnut, peanut, etc.) (Giorni et al., 2007;Passone et al., 2010). These AFs are toxic and their main adverse effects on humans are hepatocarcinoma (Qian et al., 1994; IARC, 2014), immune system deficiency (Jiang et al., 2005), reduced child growth (Gong et al., 2004) and increased risks of stillborn or newborn jaundice (Shuaib et al., 2010). To reduce those multiple effects, many countries have implemented maximum authorized levels of AFs in food and feed (Wu & Guclu, 2012). AF biosynthesis is coded by a 80 kb long DNA sequence. The latter is a cluster containing 30 putative genes characterized in both A. flavus and A. parasiticus (Yu, 2012). For structural genes, early (as aflD), medium (as aflM) and late (as aflP) genes are denominated (Fig. S1, available in the online Supplementary Material). The gene aflD encodes a reductase enzyme involved in the conversion of norsolorinic acid to averantin (Papa, 1982); aflM is required for the conversion of versicolorin A to demethylsterigmatocystin (Skory et al., 1992); and aflP encodes a methyltransferase converting sterigmatocystin to O-methylsterigmatocystin (Bhatnagar et al., 1988). Two cluster-specific regulators are also known: aflR encodes a transcription activator that binds a consensus sequence in the promoter regions of AF ...

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Reduction of aflatoxin production by Aspergillus flavus and Aspergillus parasiticus in interaction with Streptomyces

et al. 2015

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“…Primary screening for antibiotic production was undertaken on International Streptomyces Project 2 (ISP2) medium (g/l: yeast extract, 4; malt extract, 10; glucose, 4 and agar, 16 with pH 7.2) using the cross streaking method (Zitouni et al, 2005). The isolate of actinomycete was inoculated in straight line on a Petri plate containing the ISP2 medium and incubated at 30°C for seven days.…”

Section: Detection Of Antimicrobial Activitymentioning

confidence: 99%

“…Ethyl acetate was added to the supernatant in the ration 1:1 (v/v) and shaken vigorously for 2 h at room temperature. The organic solvent was then evaporated to dryness in a vacuum evaporator and the dry residue dissolved in 1 ml of methanol (Zitouni et al, 2005). The extraction from solid medium was done as follows: A dish of 9 cm in diameter containing ISP2 medium was streaked by actinomycete isolate and incubated at 30°C for 3 weeks.…”

Section: Jihani Et Al 7371mentioning

confidence: 99%

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Jihani¹,

Brodolin²,

Iraqui³

et al. 2012

Afr. J. Microbiol. Res.

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“…Therefore, the isolation of novel species of this genus from different extreme environments should provide access to new bioactive products with significant therapeutic potential and contribute to an understanding of their ecological roles. Actinobacteria diversity and their biological activities are also a prospective field of research in the Saharan soils of Algeria as several new taxa of this phylum have been reported from these soils (Bouras et al, 2014) and a significant number of antibiotic patents have recently been registered (Lamari et al, 2002a, b;Zitouni et al, 2004Zitouni et al, , 2005Badji et al, 2006Badji et al, , 2007Bouras et al, 2008;Merrouche et al, 2010;Boubetra et al, 2012) including those produced by Streptosporangium strains (Hacene et al, 1998;Boudjella et al, 2006Boudjella et al, , 2007Boudjella et al, , 2010. In the same context, a novel actinobacterium, with both antibacterial and antifungal activities, designated strain SG1 T , was isolated from a Saharan soil sample collected from Beni-Abbes, Bechar (Saoura region, south-west Algeria).…”

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Streptosporangium becharense sp. nov., an actinobacterium isolated from desert soil

Chaouch

Bouras

Mokrane

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic position of a novel actinobacterium, strain SG1 T , isolated from a Saharan soil sample collected from Beni-Abbes, Bechar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H 2 ) and MK-9(H 4 ). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C 17 : 1 !8c, iso-C 16 : 0 , 10-methyl C 17 : 0 , C 18 : 1 !9c and C 17 : 0 . 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was related most closely to 'Streptosporangium subfuscum' DSM 46724 (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181 T (98.7 %), Streptosporangium fragile DSM 43847 T (98.6 %) and Streptosporangium sandarakinum DSM 45763 T (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1 T formed a cluster with its closest relative 'S. subfuscum' DSM 46724. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1 T could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1 T should be classified as representing a novel species in the genus Streptosporangium, for which the name Streptosporangium becharense sp. nov. is proposed. The type strain is SG1 T (=DSM 46887 T =CECT 8961 T ).

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Nocardiopsis and Saccharothrix genera in Saharan soils in Algeria: Isolation, biological activities and partial characterization of antibiotics

Cited by 62 publications

References 30 publications

Reduction of aflatoxin production by Aspergillus flavus and Aspergillus parasiticus in interaction with Streptomyces

Reduction of aflatoxin production by Aspergillus flavus and Aspergillus parasiticus in interaction with Streptomyces

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Streptosporangium becharense sp. nov., an actinobacterium isolated from desert soil

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