Streptosporangium becharense sp. nov., an actinobacterium isolated from desert soil

Abstract

The taxonomic position of a novel actinobacterium, strain SG1 T , isolated from a Saharan soil sample collected from Beni-Abbes, Bechar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H 2 ) and MK-9(H 4 ). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C 17 : 1 !8c, iso-C 16 : 0 , 10-methyl C 17 : 0 , C 18 : 1 !9c and C 17 : 0 . 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was related most closely to 'Streptosporangium subfuscum' DSM 46724 (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181 T (98.7 %), Streptosporangium fragile DSM 43847 T (98.6 %) and Streptosporangium sandarakinum DSM 45763 T (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1 T formed a cluster with its closest relative 'S. subfuscum' DSM 46724. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1 T could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1 T should be classified as representing a novel species in the genus Streptosporangium, for which the name Streptosporangium becharense sp. nov. is proposed. The type strain is SG1 T (=DSM 46887 T =CECT 8961 T ).

Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. strain from a Saharan soil

“…Based on inhibition zones formation, only 45 strains confirmed their higher antagonistic potential and the strain GSBNT10 revealed a potent bioactivity against both Gram positive and negative bacteria but also against MRSA (Table 7) mainly attributed to the production of antibacterial compounds. Numerous investigations confirmed the presence of diverse culturable rare and novel actinobacteria able to produce new natural products (Lamari et al., 2002; Zitouni et al., 2004; Saker et al., 2015; Chaabane-Chaouch et al., 2016).…”

VALORIZATION OF TOMATO PLANT WASTES AND OPTIMIZATION OF GROWTH CONDITIONS FOR INDOLE-3-ACETIC ACID PRODUCTION BY Streptomyces plicatus STRAIN PT2

“…All strains have been listed belong to the microbial collection of our research laboratory (Laboratory of Biology of Microbial Systems (LBSM), ENS -Kouba, Algiers, Algeria). The actinobacterial strains have been selected on the basis of their plant's promising growth abilities (Zamoum et al, 2017;Goudjal et al, 2013); nevertheless, some of them were selected for being novel species of actinobacteria (Chaabane Chaouch et al, 2016a;Chaabane Chaouch et al, 2016b;Lahoum et al, 2016).…”

Prokaryotic Communities from Pristine Cave Environments: Biotechnological Potential with Sustainable Production

“…nov.) isolated from moonmilk deposit from the cave 'Grotte des Collemboles', Belgium [52], strain SG1 (Streptosporangium becharense sp. nov.) isolated in a Saharan soil sample collected from Algeria [3], strain AG31 (Arthrobacter psychrophenolicus sp. nov.) isolated in an Alpine ice cave, Austria [53], among many others.…”

“…However, there are microorganisms that have adapted their metabolisms to live in these environments. These ecosystems can be marine or terrestrial, for example, deserts [3,4], arctic sea ice [5,6], and deep sea [4,7,8], where different studies have been conducted to understand how microbial life is possible under these hostile conditions.…”