Nocardia sp. vanillic acid decarboxylase

Dhar, Alok; Lee, Kyung-Seon; Dhar, Kajari; Rosazza, John P. N.

doi:10.1016/j.enzmictec.2007.02.002

Cited by 19 publications

(14 citation statements)

References 39 publications

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“…Inhibitions of the VAD activity were observed in the presence of Ni 2+ , Cu 2+ , Ba 2+ and Fe 3+ , with the residual activity ranged from 82% to 20%. In contrast to the result presented here, addition of Fe 3+ had no effects on the enzymatic activity of VAD from Nocardia sp …”

Section: Resultscontrasting

confidence: 99%

“…Saturation of 80% produced the highest specific activity of 67.2 U mg −1 , which was a 9.87‐fold purification compared to that of crude extract. The results were different from those of Dhar et al who used 65% ammonium sulfate saturation to precipitate the VAD from Nocardia sp.…”

Section: Resultscontrasting

confidence: 93%

“…The 4‐hydroxybenzoate/vanillate decarboxylase from Clostridium hydroxybenzoicum exhibited a similar pH optimum of 5.6–6.2, but the optimum temperature was 50°C . As for the VAD from Nocardia sp., the pH and temperature optima were 7.0 and 30°C, respectively …”

Section: Resultsmentioning

confidence: 95%

“…The K m and V max values of the VAD were determined using a Lineweaver–Burk double reciprocal plot and calculated as 0.53 mmol L −1 and 96 U mg −1 protein, respectively, at 45°C and pH 6.5. The K m value was lower than that obtained with the purified VAD from Nocardia sp., which had a K m value of 0.8 mmol L −1 …”

Section: Resultsmentioning

confidence: 99%

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Extraction, partial purification and characterisation of vanillic acid decarboxylase fromAlicyclobacillus acidoterrestrisDSM 3923

Cai

Yuan

et al. 2015

J. Sci. Food Agric.

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Section: Resultscontrasting

confidence: 99%

Section: Resultscontrasting

confidence: 93%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Extraction, partial purification and characterisation of vanillic acid decarboxylase fromAlicyclobacillus acidoterrestrisDSM 3923

Cai

Yuan

et al. 2015

J. Sci. Food Agric.

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“…Nocardia species NRRL 5646 was initially identified as an antibiotic- producing bacterium from a garden soil sample in Osceola, Iowa (Hlavka & Bitha, 1977; Martin et al ., 1977). This organism enzymatically converts many natural and synthetic organic compounds into valuable products including flavonoids (Herath et al ., 2006; Maatooq et al ., 2005), vanillic acid (Dhar et al ., 2007) and 4-vinylphenol (Lee & Rosazza, 2002). A novel ATP/NADPH-dependent carboxylic acid reductase (CAR) was isolated from Nocardia sp.…”

mentioning

confidence: 99%

Nocardia iowensis sp. nov., an organism rich in biocatalytically important enzymes and nitric oxide synthase

Lamm

Khare

Conville

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Nocardia strain NRRL 5646, isolated from a garden soil sample in Osceola, Iowa, USA, was initially of interest as an antibiotic producer. It contained biocatalytically important enzymes and represented the first described nitric oxide synthase enzyme system in bacteria. The present polyphasic taxonomic study was undertaken to differentiate strain NRRL 5646T from related species of the genus Nocardia. Chemotaxonomic analyses included determinations of the fatty acid methyl ester profile (C 16 : 1 v6c/C 16 : 1 v7c, C 16 : 0 , C 18 : 1 v9c and C 18 : 0 10-methyl as major components), quinone [cyclo MK-8(H 4 ) as the major component], polar lipid (diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside as major components) and mycolic acid. These results supported its placement within the genus Nocardia. Biochemical testing and 16S rRNA, 65-kDa heat-shock protein (hsp65) and preprotein translocase (secA1) gene sequence analyses differentiated strain NRRL 5646 T from recognized Nocardia species. Previous studies have demonstrated that other genetic sequences (carboxylic acid reductase, Nocardia phosphopantetheinyl transferase and GTP cyclohydrolase I) from strain NRRL 5646 T can also be used to substantiate its uniqueness. The level of 16S rRNA gene sequence similarity between strain NRRL 5646 T and the type strains of Nocardia tenerifensis and Nocardia brasiliensis was 98.8 %. However, strain NRRL 5646 T could be clearly distinguished from these Nocardia species based on DNA-DNA hybridization data. Consequently, strain NRRL 5646 T is considered to represent a novel species of the genus Nocardia, for which the name Nocardia iowensis sp. nov. is proposed. The type strain is NRRL 5646 T (5UI 122540Strain NRRL 5646 T was initially identified as an antibioticproducing bacterium isolated from a garden soil sample in Osceola, Iowa, USA (Hlavka & Bitha, 1977;Martin et al., 1977). This strain showed an extraordinary diversity of biotransforming enzymes and the first bacterial nitric oxide synthase (NOS) system with possible physiological importance, making its identification to the species level important. Strain NRRL 5646 T converts many natural and synthetic organic compounds into valuable products, including flavonoids (Herath et al., 2006;Maatooq & Rosazza, 2005), vanillic acid (Dhar et al., 2007) and 4-vinylphenol (Lee &Rosazza, 2002). A novel ATP/NADPHdependent carboxylic acid reductase was isolated from strain NRRL 5646T (Li & Rosazza, 1997) and subsequently cloned (car; GenBank accession number AY495697; He et al., 2004a). The reduction system required a phosphopantetheinyl transferase (npt, GenBank accession number DQ904035; Venkitasubramanian et al., 2007). Strain NRRL 5646 T contains the first NOS system characterized in prokaryotes (Chen & Rosazza, 1995). As part of the NOS system, strain NRRL 5646 T contains guanylate cyclase for cyclic GMP synthesis (Son & Rosazza, 2000), and GTP cyclohydrolase I (gch; GenBank accession number Abbreviation: NOS, nitric oxide ...

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Bioconversion of ferulic acid into aroma compounds by newly isolated yeast strains of the Latin American biodiversity

Bettio

Zardo

Rosa

et al. 2020

Biotechnology Progress

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Nine yeast strains isolated from Latin American biodiversity were screened for ferulic acid (FA) consumption and conversion into aroma compounds such as vanillin, vanillic acid (VA), and 4-vinylguaiacol (VG). Selected strains (Rhodotorula mucilaginosa UFMG-CM-Y3647, UFMG-CM-Y2190, UFMG-CM-Y665) were evaluated in flask experiments to investigate the influence of the pH media on bioconversion and a two-step process was conducted to maximize the metabolites production. The effect of pH was found to be significantly important for FA bioconversion, as acidic conditions (pH < 6.0) improved VA accumulation, with highest production of 1.14 ± 0.02 and 1.25 ± 0.03 g/L shown by UFMG-CM-Y3647 and UFMG-CM-Y2190, respectively. The two-step process favored 4-VG production for most strains, being UFMG-CM-Y2190 the best producer, its cultures reaching 1.63 ± 0.09 g/L after 55 hr, showing a productivity of 29.59 ± 1.55 mg/(LÁhr), as glucose affected the metabolites pool and redirected yeast metabolism. R mucilaginosa UFMG-CM-Y3647 was selected for scaled-up cultivations in a 2-L bioreactor, where pH-controlled pH 5.5 and aeration of 2.5 vvm was found to be the best condition to improve VA productivity, attaining final concentrations of 1.20 ± 0.02 g/L −1 (78% molar yield) and a productivity of 40.82 ± 0.57 mg/(LÁhr).

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Nocardia sp. vanillic acid decarboxylase

Cited by 19 publications

References 39 publications

Extraction, partial purification and characterisation of vanillic acid decarboxylase fromAlicyclobacillus acidoterrestrisDSM 3923

Extraction, partial purification and characterisation of vanillic acid decarboxylase fromAlicyclobacillus acidoterrestrisDSM 3923

Nocardia iowensis sp. nov., an organism rich in biocatalytically important enzymes and nitric oxide synthase

Bioconversion of ferulic acid into aroma compounds by newly isolated yeast strains of the Latin American biodiversity

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