Nocardia beijingensis sp. nov., a novel isolate from soil.

Wang, Liming; Zhang, Yamei; Lu, Zhitang; Shi, Yanlin; Liu, Zhiheng; Maldonado, Luis A.; Goodfellow, Michael

doi:10.1099/00207713-51-5-1783

Cited by 77 publications

(50 citation statements)

References 33 publications

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“…The mortality rate can be high at 40 to 60% (11,12), and successful treatment depends on early recognition of the nocardial infection and the initiation of a specific treatment. In 2001, a new species of Nocardia, N. beijingensis, was described by Wang et al, which had been isolated from a soil sample from a sewage ditch at Xishan Mountain in Beijing (17). Strains have been isolated in Asia between 2004 and 2010 from patients with nocardiosis (5,9,10,15,16), mainly from pulmonary samples (80%) and in immunocompromised patients.…”

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Isolation of Nocardia beijingensis from a Pulmonary Abscess Reveals Human Immunodeficiency Virus Infection

et al. 2011

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A strain of Nocardia was isolated from a pulmonary abscess of a human immunodeficiency virus-infected patient in France. Comparative 16S rRNA gene sequence analysis revealed that the isolate represented a strain of Nocardia beijingensis. Antimicrobial susceptibility testing was essential to guide the clinicians to successfully treat this infection. CASE REPORTA strain of Nocardia beijingensis was isolated from a 47-yearold male who was admitted to our unit on 23 December 2010 for chest pain and a history of amoxicillin-resistant pneumonia. His main medical history was chronic sinusitis. On examination, he appeared asthenic, without fever. Physical examination concluded there was deteriorated general status, with a weight loss of 5 kg in 8 weeks. Laboratory test results were as follows: hemoglobin, 11.9 g/dl; neutrophils, 13 ϫ 10 9 /liter; lymphocytes, 1.2 ϫ 10 9 /liter; platelets, 357 ϫ 10 9 /liter; and C-reactive protein level, 241 mg/liter (normal range, Ͻ6 mg/liter). A chest radiograph disclosed mediastinal widening with a left paraaortic mass. A computed tomography (CT) scan of the chest revealed an abscess (Fig. 1). Blood cultures were negative.A bronchoalveolar lavage and a lung biopsy of the abscess were performed. In the laboratory, sputum was processed for mycobacterial study: the strain was also isolated from the mycobacterial culture medium. In a modified Ziehl-Neelsen stain, the strain was partially acid fast. The samples were tested for tuberculosis by PCR on the MTB/RIF test platform (GeneXpert; Cepheid) and were negative, except for, interestingly, one of the five probes that target the rpoB gene. Gram-stained smears revealed Gram-positive short filaments, coccoid forms, and branching rods. Primary cultures from the pulmonary abscess and a bronchoalveolar lavage on blood and chocolate agar plates, incubated at 37°C in 5% CO 2 , yielded within 3 days small, chalky white, dome-shaped rough colonies. On Coletsos medium, the colonies appeared irregular and orange colored (Fig. 2). They were Gram-positive rod-shaped organisms producing aerial hyphae. They were partially acid fast and were considered most likely to represent Nocardia spp.Antibiotic susceptibility among isolates within the genus Nocardia is unpredictable (15); therefore, we assessed susceptibility according to the guidelines of the French Society for Microbiology, using the disk diffusion method or the Etest (AB Biodisk, Solna, Sweden) on Mueller-Hinton plates. Although broth microdilution is now recommended by the Clinical and Laboratory Standards Institute (CLSI), two reports suggest that MIC determination by broth microdilution and the Etest give similar results (1, 2), making the Etest suitable and more convenient for routine work. The results were recorded after 48 h (or after 72 h if growth was insufficient after 48 h) and interpreted according to the MIC breakpoints published by the CLSI. However, the Etest method was not available for every antibiotic in our laboratory. Therefore, the agar disk diffusion method on Mueller-Hinton ...

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Isolation of Nocardia beijingensis from a Pulmonary Abscess Reveals Human Immunodeficiency Virus Infection

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“…Nitrogen sources are not utilized. Kinoshita et al (2001), Kudo et al (1988), Maldonado et al (2000), Yassin et al (2001), Wang et al (2001) …”

Section: Description Of Nocardia Araoensis Sp Novmentioning

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Nocardia araoensis sp. nov. and Nocardia pneumoniae sp. nov., isolated from patients in Japan

Kageyama

Yazawa

Mukai

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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Two actinomycete strains isolated from two patients with lung nocardiosis between 1995 and 1997 in Japan were assigned to novel species of the genus Nocardia based on morphological and chemical criteria. Comparative 16S rRNA gene sequence analysis of the two strains revealed that they belong to the genus Nocardia and are most closely related to the species Nocardia beijingensis. Determination of DNA–DNA relatedness indicated that these strains could be assigned to two novel species. Based on their phenotypic and phylogenetic characters, two novel species of the genus Nocardia are proposed: Nocardia araoensis sp. nov. for IFM 0575T (=NBRC 100135T=JCM 12118T=DSM 44729T) and Nocardia pneumoniae sp. nov. for IFM 0784T (=NBRC 100136T=JCM 12119T=DSM 44730T).

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“…For instance, the 16S rDNA of N. beijingensis is 97?6 and 97?5 % similar to that of N. brasiliensis and N. farcinica, respectively, though the distinction between N. beijingensis and the two species has been convincingly illustrated by different characters and did not require the determination of DNA hybridization values (Wang et al, 2001). Similarly, Nocardia vaccinii and Nocardia africana share 98?5-98?7 % 16S rDNA sequence similarity (Hamid et al, 2001).…”

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Nocardia puris sp. nov.

Yassin

Sträubler

Schümann

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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A bacterial isolate (IMMIB R-145 T ) from a human abscess was subjected to a polyphasic taxonomic study. Chemotaxonomic investigations revealed the isolate to have cell wall chemotype IV and mycolic acids consistent with characteristics of the genus Nocardia. Comparative 16S rRNA gene sequencing showed that the isolate constituted a distinct subline within the genus Nocardia, displaying greater than 1?7 % sequence divergence with established species. However, a DNA-DNA hybridization study demonstrated unambiguously that the isolate was genealogically distinct from closely related species, namely, Nocardia abscessus, Nocardia cyriacigeorgica and Nocardia farcinica, with which it showed high levels of 16S rDNA sequence similarity (97?8, 97?9 and 98?3 %, respectively). Based on phenotypic and phylogenetic data, it is proposed that this isolate be classified as a new species of the genus Nocardia, for which the name Nocardia puris (type strain IMMIB R-145 T =DSM 44599 T =NRRL B-24204 T ) is proposed.

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Nocardia beijingensis sp. nov., a novel isolate from soil.

Cited by 77 publications

References 33 publications

Isolation of Nocardia beijingensis from a Pulmonary Abscess Reveals Human Immunodeficiency Virus Infection

Isolation of Nocardia beijingensis from a Pulmonary Abscess Reveals Human Immunodeficiency Virus Infection

Nocardia araoensis sp. nov. and Nocardia pneumoniae sp. nov., isolated from patients in Japan

Nocardia puris sp. nov.

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