No induction of chromosomal aberrations in Chinese hamster ovary cells by chrysophanol

Mengs, U.; Schuler, Detlef; Marshall, Richard

doi:10.1016/s1383-5718(01)00150-4

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…AMPK pathway is a "cellular fuel gage" which functions in rapid changes for the mastery of fatty acid metabolism. 20 The existing CPA is a quite safe drug, even the high dose (30 μg/mL) of which has no significant disturbance in chromosomal aberrations, 49 and the accumulation of CPA metabolites is quite low in the liver and kidney. 50 Our results also demonstrated that CPA was well tolerated as evidenced by no changes in daily behaviour, survival rate and body weight in rats.…”

Section: Discussionmentioning

confidence: 99%

Chrysophanic acid shifts the differentiation tendency of BMSCs to prevent alcohol‐induced osteonecrosis of the femoral head

Liu

Zhu

et al. 2020

Cell Proliferation

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Objectives Osteonecrosis of the femoral head (ONFH), largely caused by alcohol abuse, is a refractory bone disease characterized by the impaired capacity of osteogenic differentiation of bone mesenchymal stem cells (BMSCs), as well as the disordered adipocyte accumulation. Chrysophanic acid (CPA) is a natural anthraquinone which has lipid regulation and bone protection capacity. The aim of this study was to reveal the potential function of CPA and the underlying mechanisms for the alcohol‐induced ONFH. Materials and Methods The effects of alcohol and CPA on BMSCs were investigated by cell proliferation, induced differentiation assays and immunofluorescent staining. Meanwhile, the function of PI3K/AKT and AMPK pathway was investigated in the process of osteogenic and adipogenic differentiation, respectively. Furthermore, we established the rat model of alcohol‐induced ONFH to reveal the pharmacotherapeutic effect of CPA in vivo using radiographical and histopathological methods. Results In vitro, alcohol significantly inhibited the proliferation and osteogenic differentiation of BMSCs but stimulated the adipogenic differentiation. However, CPA could counteract the anti‐osteogenesis of alcohol partly via PI3K/AKT pathway and retard the promotion of alcohol‐induced adipogenesis via AMPK pathway. In vivo, radiographical and histopathological findings showed that CPA could alleviate alcohol‐induced ONFH and substantially restore the bone volume. Conclusions We demonstrated that CPA ameliorated alcohol‐induced ONFH possibly via regulating the differentiation tendency of BMSCs. Hence, CPA may become a beneficial herb extract to alleviate alcohol‐induced ONFH.

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Section: Discussionmentioning

confidence: 99%

Chrysophanic acid shifts the differentiation tendency of BMSCs to prevent alcohol‐induced osteonecrosis of the femoral head

Liu

Zhu

et al. 2020

Cell Proliferation

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“…investigated the capability of chrysophanol to cause chromosomal aberrations in the Chinese hamster ovary cell assay. There were no significant increases in chromosomal aberrations when chrysophanol was tested up to its limit of solubility with or without metabolic activation (30 μg/ml) . Moreover, the accumulation of chrysophanol metabolites in the liver and kidney was very low .…”

Section: Pharmacologymentioning

confidence: 92%

“…There were no significant increases in chromosomal aberrations when chrysophanol was tested up to its limit of solubility with or without metabolic activation (30 lg/ml). [76] Moreover, the accumulation of chrysophanol metabolites in the liver and kidney was very low. [77] Similarly, in the primary rat hepatocytes and HepG2 cell cytotoxicity experiments, the toxicity of chrysophanol was the lowest of the five species (rhein, emodin, aloe-emodin, physcion and chrysophanol).…”

Section: Toxicitymentioning

confidence: 99%

Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Xie

Tang

Song

et al. 2019

Journal of Pharmacy and Pharmacology

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Objective Chrysophanol is a natural anthraquinone, also known as chrysophanic acid and 1,8‐dihydroxy‐3‐methyl‐anthraquinone. It has been widely used in the food and pharmaceutical fields. This review is intended to provide a comprehensive overview of the pharmacology, toxicity and pharmacokinetic researches of chrysophanol. Key finding Information on chrysophanol was collected from the Internet database PubMed, Elsevier, ResearchGate, Web of Science, Wiley Online Library and Europe PM using a combination of keywords including ‘pharmacology’, ‘toxicology’ and ‘pharmacokinetics’. The literature we collected included from January 2010 to June 2019. Chrysophanol has a wide spectrum of pharmacological effects, including anticancer, antioxidation, neuroprotection, antibacterial and antiviral, and regulating blood lipids. However, chrysophanol has obvious hepatotoxicity and nephrotoxicity, and pharmacokinetics indicate that the use of chrysophanol in combination with other drugs can reduce toxicity and enhance efficacy. Summary Chrysophanol can be used in many diseases. Future research directions include how the concentration of chrysophanol affects pharmacological effects and toxicity; the mechanism of synergy between chrysophanol and other drugs.

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“…This study concluded that chrysophanol binds to DNA in a similar manner as ethidium bromide, mitoxanthrone, adriamycin, but it is not potentially toxic as the others are; the saturation value of drug binding for chrysophanol was 0.53 whereas it was 3.31, 10.58, and 14.70 for mitoxanthrone, adriamycin, and ethidium bromide, respectively. Mengs et al [130] investigated the chromosomal aberration potential of chrysophanol in Chinese hamster ovary (CHO), and concluded that it had no clastogenic potential up to its solubility limit. The mutagenic effect of chrysophanol on other mammalian cells, rat hepatocytes, and v79 was also investigated in the unscheduled DNA synthesis test and hypoxanthine-guanine phosphoribosyl transferase test.…”

Section: Toxicologymentioning

confidence: 99%

Chrysophanol: A Natural Anthraquinone with Multifaceted Biotherapeutic Potential

et al. 2019

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Chrysophanol is a unique anthraquinone having broad-spectrum therapeutic potential along with ecological importance. It is the first polyketide that has been reported to be biosynthesized in an organism-specific manner. The traditional Chinese and Korean medicinal systems provide evidence of the beneficial effects of chrysophanol on human health. The global distribution of chrysophanol encountered in two domains of life (bacteria and eukaryota) has motivated researchers to critically evaluate the properties of this compound. A plethora of literature is available on the pharmacological properties of chrysophanol, which include anticancer, hepatoprotective, neuroprotective, anti-inflammatory, antiulcer, and antimicrobial activities. However, the pharmacokinetics and toxicity studies on chrysophanol demand further investigations for it to be used as a drug. This is the first comprehensive review on the natural sources, biosynthetic pathways, and pharmacology of chrysophanol. Here we reviewed recent advancements made on the pharmacokinetics of the chrysophanol. Additionally, we have highlighted the knowledge gaps of its mechanism of action against diseases and toxicity aspects.

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No induction of chromosomal aberrations in Chinese hamster ovary cells by chrysophanol

Cited by 9 publications

References 8 publications

Chrysophanic acid shifts the differentiation tendency of BMSCs to prevent alcohol‐induced osteonecrosis of the femoral head

Chrysophanic acid shifts the differentiation tendency of BMSCs to prevent alcohol‐induced osteonecrosis of the femoral head

Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Chrysophanol: A Natural Anthraquinone with Multifaceted Biotherapeutic Potential

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