NO GAMETOPHORES 2 Is a Novel Regulator of the 2D to 3D Growth Transition in the Moss Physcomitrella patens

Moody, Laura A.; Kelly, Steven L.; Clayton, Roxaana; Weeks, Zoe; Emms, David; Langdale, Jane A.

doi:10.1016/j.cub.2020.10.077

Cited by 31 publications

(43 citation statements)

References 52 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5C). TheΔPpHCT lines phenocopied Δ PpCYP98 mutants(Renault et al, 2017) and newly reported PpHCT loss-of-function mutant Ppnog2-R(Moody et al, 2020), characterized by defective gametophore development (Fig. 5D-F, Fig.…”

mentioning

confidence: 70%

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Kriegshauser

Knosp

Grienenberger

et al. 2020

Preprint

View full text Add to dashboard Cite

The plant phenylpropanoid pathway generates a major class of specialized metabolites and precursors of essential extracellular polymers that initially appeared upon plant terrestrialization. Despite its evolutionary significance, little is known about the complexity and function of this major metabolic pathway in extant bryophytes, the ancestors of which were the first land plants. Here, we report that the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE (HCT) gene, which plays a critical function in the phenylpropanoid pathway during seed plant development, is functionally conserved in Physcomitrium (Physcomitrella) patens, in the moss lineage of bryophytes. Phylogenetic analysis indicates that bona fide HCT function emerged in the progenitor of embryophytes. In vitro enzyme assays, moss phenolic pathway reconstitution in yeast and in planta gene inactivation coupled to targeted metabolic profiling collectively indicate that P. patens HCT (PpHCT), similar to tracheophyte HCT orthologs, uses shikimate as a native acyl acceptor to produce a p-coumaroyl-5-O-shikimate intermediate. Phenotypic and metabolic analyses of loss-of-function mutants show that PpHCT is necessary for the production of caffeate derivatives, including previously reported caffeoyl-threonate esters, and for the formation of an intact cuticle. Deep conservation of HCT function in embryophytes is further suggested by the ability of HCT genes from P. patens and the liverwort Marchantia polymorpha to complement an Arabidopsis thaliana CRISPR/Cas9 hct mutant, and by the presence of phenolic esters of shikimate in representative species of the three major bryophyte lineages.One sentence summaryMultidisciplinary study of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE (HCT) gene in Physcomitrium (Physcomitrella) patens points to its functional conservation in embryophytes.

show abstract

mentioning

confidence: 70%

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Kriegshauser

Knosp

Grienenberger

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The regulon comprises several genes encoding biosynthesis ( IPT3 Lindner et al, 2014), activation ( LOG Kuroha et al, 2009), degradation ( CKX Schwartzenberg et al, 2007), transport ( ENT , ABCG Borghi et al, 2015) perception ( CHK1 , CHK2 Schwartzenberg et al, 2016) and transduction ( ARR To and Kieber 2008) of cytokinins. In addition, the MYB circuit of the regulon also is predicted to induce cytokinin-responsive genes like NO GAMETOPHORES 2 ( NOG2 Moody et al, 2021), whose loss-of-function mutant similar to DEK1 also displays an overbudding phenotype. Consistently, the exogenous application of cytokinin (Schwartzenberg et al, 2007) and cytokinin-overproducing mutants (Schulz et al, 2000) also result in an overbudding phenotype.…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned above, the regulon also comprises essential components of the CLAVATA (CLV) peptide and receptor-like kinase pathway that has been shown to control cell fates and division planes of land plant apical stem cells (Fletcher et al, 1999; Whitewoods et al, 2018; Hirakawa et al, 2019) via CLV3/EMBRYO SURROUNDING REGION-Related (CLE Yamaguchi et al, 2016) peptide hormones which are perceived and transmitted to downstream signalling cascades via CLV1-type receptor-like kinases (Hazak and Hardtke 2016). Several studies in both Arabidopsis (Johnson et al, 2008) and Physcomitrella (Whitewoods et al, 2018; Moody et al, 2018; Moody et al, 2021) already have identified parallels in mutant phenotypes and expression patterns and have proposed models locating the CLV signalling pathway somewhere downstream of DEK1 and the moss APBs. The predicted overbudding-up regulon identified here, now provides us with a robust explanation for these connections.…”

Section: Resultsmentioning

confidence: 99%

Calpain DEK1 acts as a developmental switch gatekeeping cell fate transitions

Demko

Belova

Messerer

et al. 2021

Preprint

View full text Add to dashboard Cite

Calpains are cysteine proteases that control cell fate transitions. Although calpains are viewed as modulatory proteases displaying severe, pleiotropic phenotypes in eukaryotes, human calpain targets are also directed to the N-end rule degradatory pathway. Several of these destabilized targets are transcription factors, hinting at a gene regulatory role. Here, we analyze the gene regulatory networks of Physcomitrium patens and characterize the regulons that are deregulated in DEK1 calpain mutants. Predicted cleavage patterns of regulatory hierarchies in the five DEK1-controlled subnetworks are consistent with the gene's pleiotropy and the regulatory role in cell fate transitions targeting a broad spectrum of functions. Network structure suggests DEK1-gated sequential transition between cell fates in 2D to 3D development. We anticipate that both our method combining phenotyping, transcriptomics and data science to dissect phenotypic traits and our model explaining the calpain's role as a switch gatekeeping cell fate transitions will inform biology beyond plant development.

show abstract

“…Specification of the shoot initial cell requires both cytokinin and auxin (Ashton et al, 1978;Cove et al, 2006;Bennett et al, 2014). Factors including DEFECTIVE KERNEL 1 (DEK1), NO GAMETOPHORES 1 and 2 (NOG1 and 2) RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2), and CLAVATA (CLV) function through APETALA2-type (AP2type) transcription factors to control the frequency of shoot initial cells (Aoyama et al, 2012;Perroud et al, 2014;Moody et al, 2018Moody et al, , 2021Whitewoods et al, 2018;Demko et al, 2021;Nemec Venza et al, 2021). In P. patens, a shoot initial cell undergoes several rounds of stereotypic, oblique cell divisions that lead to the formation of a tetrahedral shoot apical cell, marking the transition from a so-called 2D to 3D growth mode (Figure 2A; Harrison et al, 2009).…”

Section: Leaf Initiationmentioning

confidence: 99%

“…In P. patens, a shoot initial cell undergoes several rounds of stereotypic, oblique cell divisions that lead to the formation of a tetrahedral shoot apical cell, marking the transition from a so-called 2D to 3D growth mode (Figure 2A; Harrison et al, 2009). These divisions are also regulated by DEK1, CLV, NOG1, and NOG2 genes (Perroud et al, 2014;Moody et al, 2018Moody et al, , 2021Whitewoods et al, 2018;Nemec Venza et al, 2021), and precisely fulfilled by mitotic spindle orientation regulators, including microtubule-associated protein TARGETING FACTOR FOR Xklp2 and SABRE (Kosetsu et al, 2017;Kozgunova et al, 2020;Cheng and Bezanilla, 2021). Additionally, SOSEKI proteins might also be involved in apical cell identity specification and division (van Dop et al, 2020).…”

Section: Leaf Initiationmentioning

confidence: 99%

Leaf Morphogenesis: Insights From the Moss Physcomitrium patens

et al. 2021

View full text Add to dashboard Cite

Specialized photosynthetic organs have appeared several times independently during the evolution of land plants. Phyllids, the leaf-like organs of bryophytes such as mosses or leafy liverworts, display a simple morphology, with a small number of cells and cell types and lack typical vascular tissue which contrasts greatly with flowering plants. Despite this, the leaf structures of these two plant types share many morphological characteristics. In this review, we summarize the current understanding of leaf morphogenesis in the model moss Physcomitrium patens, focusing on the underlying cellular patterns and molecular regulatory mechanisms. We discuss this knowledge in an evolutionary context and identify parallels between moss and flowering plant leaf development. Finally, we propose potential research directions that may help to answer fundamental questions in plant development using moss leaves as a model system.

show abstract

NO GAMETOPHORES 2 Is a Novel Regulator of the 2D to 3D Growth Transition in the Moss Physcomitrella patens

Cited by 31 publications

References 52 publications

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Calpain DEK1 acts as a developmental switch gatekeeping cell fate transitions

Leaf Morphogenesis: Insights From the Moss Physcomitrium patens

Contact Info

Product

Resources

About